Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement

Global warming has become a significant public health concern, with intensive livestock farming as a major contributor. To mitigate greenhouse gas emissions, strategies such as manipulating the ruminal environment with dietary additives are essential. This study evaluated Moringa oleifera, a globall...

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Autores: Grijalva-Hinojos, Alicia Alejandra, Arnau, Vicente, Diaz, Wladimiro, Piquer, Samuel, Diaz-Plascencia, Daniel, Castillo-Castillo, Yamicela, Dominguez-Viveros, Joel, Ordonez-Baquera, Perla Lucia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:dnet:r-fisabio___::3acbe6830447d809338e865e2e298ce1
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/20933
Access Level:acceso abierto
Palabra clave:metatranscriptomics
rumen microbiome
Moringa oleifera
methane
fermentation
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spelling Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary SupplementGrijalva-Hinojos, Alicia AlejandraArnau, VicenteDiaz, WladimiroPiquer, SamuelDiaz-Plascencia, DanielCastillo-Castillo, YamicelaDominguez-Viveros, JoelOrdonez-Baquera, Perla Luciametatranscriptomicsrumen microbiomeMoringa oleiferamethanefermentationGlobal warming has become a significant public health concern, with intensive livestock farming as a major contributor. To mitigate greenhouse gas emissions, strategies such as manipulating the ruminal environment with dietary additives are essential. This study evaluated Moringa oleifera, a globally widespread tree with antioxidant, multivitamin, protein-rich, and anti-inflammatory properties, as a feed additive. Rumen fluid was collected from three Pelibuey sheep, homogenized, and subjected to an in vitro fermentation study for 48 h with three alfalfa/moringa ratio treatments: T0 Control (100:0), T1 Low (85:15), and T2 High (70:30). Total RNA was extracted, followed by high-definition sequencing of the metatranscriptome. The sequencing yielded approximately 456 million sequences. A total of 117 phyla were identified and approximately 1300 genera were mapped. Predominant phylum differed by treatment: T0, Firmicutes; T1, Proteobacteria; and T2 with Synergistetes, at least one sample per treatment. Archaea were nearly absent in T1, which explains a statistically significant decrease in methane production. In the Gene Set Enrichment Analysis (GSEA), it was observed that one of the metabolic pathways with a statistically significant difference (p-value < 0.05) was that of methane, specifically in the low moringa treatment (T1) compared to the control (T0). From the functional analysis, differentially expressed enzymes were identified, some of which are involved in the methane metabolic pathway, such as formate dehydrogenase (EC 1.17.1.9) and glycine hydroxymethyltransferase (EC 2.1.2.1), which are intermediates in methane formation. These results suggest that 15% Moringa oleifera supplementation alters ruminal microbiota, reduces archaeal activity, and suppresses methane-related pathways. These findings provide molecular evidence supporting the potential of M. oleifera as a methane mitigation strategy in ruminant nutrition.MDPI2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/20933Fermentation-BaselISSN: 23115637reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:dnet:r-fisabio___::3acbe6830447d809338e865e2e298ce12026-06-11T12:45:17Z
dc.title.none.fl_str_mv Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
title Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
spellingShingle Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
Grijalva-Hinojos, Alicia Alejandra
metatranscriptomics
rumen microbiome
Moringa oleifera
methane
fermentation
title_short Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
title_full Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
title_fullStr Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
title_full_unstemmed Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
title_sort Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement
dc.creator.none.fl_str_mv Grijalva-Hinojos, Alicia Alejandra
Arnau, Vicente
Diaz, Wladimiro
Piquer, Samuel
Diaz-Plascencia, Daniel
Castillo-Castillo, Yamicela
Dominguez-Viveros, Joel
Ordonez-Baquera, Perla Lucia
author Grijalva-Hinojos, Alicia Alejandra
author_facet Grijalva-Hinojos, Alicia Alejandra
Arnau, Vicente
Diaz, Wladimiro
Piquer, Samuel
Diaz-Plascencia, Daniel
Castillo-Castillo, Yamicela
Dominguez-Viveros, Joel
Ordonez-Baquera, Perla Lucia
author_role author
author2 Arnau, Vicente
Diaz, Wladimiro
Piquer, Samuel
Diaz-Plascencia, Daniel
Castillo-Castillo, Yamicela
Dominguez-Viveros, Joel
Ordonez-Baquera, Perla Lucia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv metatranscriptomics
rumen microbiome
Moringa oleifera
methane
fermentation
topic metatranscriptomics
rumen microbiome
Moringa oleifera
methane
fermentation
description Global warming has become a significant public health concern, with intensive livestock farming as a major contributor. To mitigate greenhouse gas emissions, strategies such as manipulating the ruminal environment with dietary additives are essential. This study evaluated Moringa oleifera, a globally widespread tree with antioxidant, multivitamin, protein-rich, and anti-inflammatory properties, as a feed additive. Rumen fluid was collected from three Pelibuey sheep, homogenized, and subjected to an in vitro fermentation study for 48 h with three alfalfa/moringa ratio treatments: T0 Control (100:0), T1 Low (85:15), and T2 High (70:30). Total RNA was extracted, followed by high-definition sequencing of the metatranscriptome. The sequencing yielded approximately 456 million sequences. A total of 117 phyla were identified and approximately 1300 genera were mapped. Predominant phylum differed by treatment: T0, Firmicutes; T1, Proteobacteria; and T2 with Synergistetes, at least one sample per treatment. Archaea were nearly absent in T1, which explains a statistically significant decrease in methane production. In the Gene Set Enrichment Analysis (GSEA), it was observed that one of the metabolic pathways with a statistically significant difference (p-value < 0.05) was that of methane, specifically in the low moringa treatment (T1) compared to the control (T0). From the functional analysis, differentially expressed enzymes were identified, some of which are involved in the methane metabolic pathway, such as formate dehydrogenase (EC 1.17.1.9) and glycine hydroxymethyltransferase (EC 2.1.2.1), which are intermediates in methane formation. These results suggest that 15% Moringa oleifera supplementation alters ruminal microbiota, reduces archaeal activity, and suppresses methane-related pathways. These findings provide molecular evidence supporting the potential of M. oleifera as a methane mitigation strategy in ruminant nutrition.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/20933
url https://fisabio.portalinvestigacion.com/publicaciones/20933
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Fermentation-Basel
ISSN: 23115637
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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