Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface

The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for i...

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Autores: Díez Orejas, Rosalía María, Casarrubios Molina, Laura, Feito Castellano, María José, Rojo, J. M., Vallet Regí, María Dulce Nombre, Arcos Navarrete, Daniel, Portolés Pérez, María Teresa
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7794
Acesso em linha:https://hdl.handle.net/20.500.14352/7794
Access Level:acceso abierto
Palavra-chave:539.2:620.1
577.1
Mesoporous nanospheres
Macrophage
Candida albicans
Immune response
Nanomaterial
Phagocytosis
Bioquímica (Química)
Materiales
3312 Tecnología de Materiales
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repository_id_str
spelling Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interfaceDíez Orejas, Rosalía MaríaCasarrubios Molina, LauraFeito Castellano, María JoséRojo, J. M.Vallet Regí, María Dulce NombreArcos Navarrete, DanielPortolés Pérez, María Teresa539.2:620.1577.1Mesoporous nanospheresMacrophageCandida albicansImmune responseNanomaterialPhagocytosisBioquímica (Química)Materiales3312 Tecnología de MaterialesThe use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2-CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers(CD80 expression and interleukin 6 secretion) were also evaluated. Significant decreases of CD80+ macrophage percentage and interleukin 6 secretion were observed after 30 min, indicating that the simultaneous incorporation of NanoMBG and fungus favors the macrophage non-inflammatory phenotype. The present study evidences that the uptake of these nanospheres in all the studied conditions does not alter the macrophage function. Moreover, intracellular FITC-NanoMBGs induce a transitory increase of the fungal phagocytosis by macrophages at MOI 1 and after a short time of interaction. In the competition assays, as the intracellular fungus quantity increased, the intracellular FITC-NanoMBG content decreased in a MOI- and time-dependent manner. These results have confirmed that macrophages clearly distinguish between inert material and the live yeast in a dynamic intracellular incorporation. Furthermore, macrophage phagocytosis is a critical determinant to know their functional state and a valuable parameter to study the nanomaterial / macrophages / Candida albicans interface.ElsevierUniversidad Complutense de Madrid20212021-03-2020212021-03-20journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7794reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/77942026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
title Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
spellingShingle Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
Díez Orejas, Rosalía María
539.2:620.1
577.1
Mesoporous nanospheres
Macrophage
Candida albicans
Immune response
Nanomaterial
Phagocytosis
Bioquímica (Química)
Materiales
3312 Tecnología de Materiales
title_short Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
title_full Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
title_fullStr Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
title_full_unstemmed Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
title_sort Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
dc.creator.none.fl_str_mv Díez Orejas, Rosalía María
Casarrubios Molina, Laura
Feito Castellano, María José
Rojo, J. M.
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author Díez Orejas, Rosalía María
author_facet Díez Orejas, Rosalía María
Casarrubios Molina, Laura
Feito Castellano, María José
Rojo, J. M.
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author_role author
author2 Casarrubios Molina, Laura
Feito Castellano, María José
Rojo, J. M.
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 539.2:620.1
577.1
Mesoporous nanospheres
Macrophage
Candida albicans
Immune response
Nanomaterial
Phagocytosis
Bioquímica (Química)
Materiales
3312 Tecnología de Materiales
topic 539.2:620.1
577.1
Mesoporous nanospheres
Macrophage
Candida albicans
Immune response
Nanomaterial
Phagocytosis
Bioquímica (Química)
Materiales
3312 Tecnología de Materiales
description The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2-CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers(CD80 expression and interleukin 6 secretion) were also evaluated. Significant decreases of CD80+ macrophage percentage and interleukin 6 secretion were observed after 30 min, indicating that the simultaneous incorporation of NanoMBG and fungus favors the macrophage non-inflammatory phenotype. The present study evidences that the uptake of these nanospheres in all the studied conditions does not alter the macrophage function. Moreover, intracellular FITC-NanoMBGs induce a transitory increase of the fungal phagocytosis by macrophages at MOI 1 and after a short time of interaction. In the competition assays, as the intracellular fungus quantity increased, the intracellular FITC-NanoMBG content decreased in a MOI- and time-dependent manner. These results have confirmed that macrophages clearly distinguish between inert material and the live yeast in a dynamic intracellular incorporation. Furthermore, macrophage phagocytosis is a critical determinant to know their functional state and a valuable parameter to study the nanomaterial / macrophages / Candida albicans interface.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-03-20
2021
2021-03-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7794
url https://hdl.handle.net/20.500.14352/7794
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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