Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii

Due to the wide range of applications of cerium oxide nanoparticles (CeO2NPs), a risk assessment of their biological effects using environmentally relevant species becomes highly important. There are contradictory reports on the effects of CeO2NPs, which may be related to the use of different types...

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Autores: Pulido Reyes, Gerardo, Briffa, Sophie Marie, Hurtado Gallego, Jara, Yudina, Tetyana, Leganés Nieto, Francisco, Puntes, Victor, Valsami-Jones, Eugenia, Rosal, Roberto, Fernández Piñas, Francisca
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/709951
Acceso en línea:http://hdl.handle.net/10486/709951
https://dx.doi.org/10.1039/c9en00363k
Access Level:acceso abierto
Palabra clave:Nanocrystal
Titanium Dioxide Nanoparticle
Ecotoxicity
Biología y Biomedicina / Biología
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spelling Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtiiPulido Reyes, GerardoBriffa, Sophie MarieHurtado Gallego, JaraYudina, TetyanaLeganés Nieto, FranciscoPuntes, VictorValsami-Jones, EugeniaRosal, RobertoFernández Piñas, FranciscaNanocrystalTitanium Dioxide NanoparticleEcotoxicityBiología y Biomedicina / BiologíaDue to the wide range of applications of cerium oxide nanoparticles (CeO2NPs), a risk assessment of their biological effects using environmentally relevant species becomes highly important. There are contradictory reports on the effects of CeO2NPs, which may be related to the use of different types of nanoparticles (NPs) and coatings. CeO2NPs may act as an oxidant causing toxicity or as an antioxidant able to scavenge free radicals. As a consequence of such complexity, the toxicological behaviour of these NPs is still poorly understood. Moreover, little is known about the internalization process of CeO2NPs in algae. There is evidence of CeO2NP-internalization by the green alga Chlamydomonas reinhardtii, but the mechanism and route of uptake are still unknown. In this study, we used uncoated and different polyvinylpyrrolidone (PVP)-coated CeO2NPs with the aim of identifying their toxicological mechanisms to C. reinhardtii and exploring their possible internalization. Our results showed that PVP coated-CeO2NPs significantly increased the formation of reactive oxygen species in exposed cells, indicating that oxidative stress is an important toxicity mechanism for these particles. Direct contact and damage of the cellular membrane was identified as the mechanism causing the toxicity of uncoated NPs. From experiments with endocytosis inhibitors, clathrin-dependent endocytosis was revealed as the main internalization route for all NPs. However, as uncoated CeO2NPs led to severe cellular membrane damage, direct passage of NPs through membrane holes could not be discarded. To our knowledge, this is the first report with evidence of direct linking between NP internalization and a specific endocytic pathway. The results presented here will help to unravel the toxicological mechanism and behaviour of CeO2NPs and provide input information for the environmental health and safety assessment of CeO2NPsThis research was supported by CTM2013-45775-C2-1,2-R and CTM2016-74927-C2-1,2-R grants from MINECO. NanoMILE (Grant Agreement no 310451 to EVJ & SMB) and the Endeavour Scholarship Scheme (Group B) (to SMB) are acknowledgedRoyal Society of ChemistryDepartamento de BiologíaFacultad de Ciencias20192019-05-10research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/709951https://dx.doi.org/10.1039/c9en00363kreponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7099512026-06-23T12:46:27Z
dc.title.none.fl_str_mv Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
title Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
spellingShingle Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
Pulido Reyes, Gerardo
Nanocrystal
Titanium Dioxide Nanoparticle
Ecotoxicity
Biología y Biomedicina / Biología
title_short Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
title_full Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
title_fullStr Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
title_full_unstemmed Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
title_sort Internalization and toxicological mechanisms of uncoated and PVP-coated cerium oxide nanoparticles in the freshwater alga: Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Pulido Reyes, Gerardo
Briffa, Sophie Marie
Hurtado Gallego, Jara
Yudina, Tetyana
Leganés Nieto, Francisco
Puntes, Victor
Valsami-Jones, Eugenia
Rosal, Roberto
Fernández Piñas, Francisca
author Pulido Reyes, Gerardo
author_facet Pulido Reyes, Gerardo
Briffa, Sophie Marie
Hurtado Gallego, Jara
Yudina, Tetyana
Leganés Nieto, Francisco
Puntes, Victor
Valsami-Jones, Eugenia
Rosal, Roberto
Fernández Piñas, Francisca
author_role author
author2 Briffa, Sophie Marie
Hurtado Gallego, Jara
Yudina, Tetyana
Leganés Nieto, Francisco
Puntes, Victor
Valsami-Jones, Eugenia
Rosal, Roberto
Fernández Piñas, Francisca
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Nanocrystal
Titanium Dioxide Nanoparticle
Ecotoxicity
Biología y Biomedicina / Biología
topic Nanocrystal
Titanium Dioxide Nanoparticle
Ecotoxicity
Biología y Biomedicina / Biología
description Due to the wide range of applications of cerium oxide nanoparticles (CeO2NPs), a risk assessment of their biological effects using environmentally relevant species becomes highly important. There are contradictory reports on the effects of CeO2NPs, which may be related to the use of different types of nanoparticles (NPs) and coatings. CeO2NPs may act as an oxidant causing toxicity or as an antioxidant able to scavenge free radicals. As a consequence of such complexity, the toxicological behaviour of these NPs is still poorly understood. Moreover, little is known about the internalization process of CeO2NPs in algae. There is evidence of CeO2NP-internalization by the green alga Chlamydomonas reinhardtii, but the mechanism and route of uptake are still unknown. In this study, we used uncoated and different polyvinylpyrrolidone (PVP)-coated CeO2NPs with the aim of identifying their toxicological mechanisms to C. reinhardtii and exploring their possible internalization. Our results showed that PVP coated-CeO2NPs significantly increased the formation of reactive oxygen species in exposed cells, indicating that oxidative stress is an important toxicity mechanism for these particles. Direct contact and damage of the cellular membrane was identified as the mechanism causing the toxicity of uncoated NPs. From experiments with endocytosis inhibitors, clathrin-dependent endocytosis was revealed as the main internalization route for all NPs. However, as uncoated CeO2NPs led to severe cellular membrane damage, direct passage of NPs through membrane holes could not be discarded. To our knowledge, this is the first report with evidence of direct linking between NP internalization and a specific endocytic pathway. The results presented here will help to unravel the toxicological mechanism and behaviour of CeO2NPs and provide input information for the environmental health and safety assessment of CeO2NPs
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-05-10
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/709951
https://dx.doi.org/10.1039/c9en00363k
url http://hdl.handle.net/10486/709951
https://dx.doi.org/10.1039/c9en00363k
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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