Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation
This is the peer reviewed version of the following article: [Juan C. Begara-Morales, Beatriz Sánchez-Calvo, Mounira Chaki, Capilla Mata-Pérez, Raquel Valderrama, María N. Padilla, Javier López-Jaramillo, Francisco Luque, Francisco J. Corpas, Juan B. Barroso, Differential molecular response of monode...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universidad de Jaén |
| Repositorio: | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| OAI Identifier: | oai:ruja.ujaen.es:10953/4397 |
| Acceso en línea: | https://academic.oup.com/jxb/article/66/19/5983/697589?login=false https://hdl.handle.net/10953/4397 |
| Access Level: | acceso abierto |
| Palabra clave: | Glutathione reductase Monodehydroascorbate reductase Nitration Nitric oxide Peroxynitrite Reactive nitrogen species Salinity S-nitrosylation S-nitrosoglutathione. |
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| dc.title.none.fl_str_mv |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| title |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| spellingShingle |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation Begara-Morales, Juan Carlos Glutathione reductase Monodehydroascorbate reductase Nitration Nitric oxide Peroxynitrite Reactive nitrogen species Salinity S-nitrosylation S-nitrosoglutathione. |
| title_short |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| title_full |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| title_fullStr |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| title_full_unstemmed |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| title_sort |
Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation |
| dc.creator.none.fl_str_mv |
Begara-Morales, Juan Carlos Sánchez-Calvo, Beatriz Chaki, Mounira Mata-Pérez, Capilla Valderrama, Raquel Padilla-Serrano, María Nieves López-Jaramillo, Javier Luque-Vázquez, Francisco Corpas, Francisco Javier Barroso-Albarracín, Juan Bautista |
| author |
Begara-Morales, Juan Carlos |
| author_facet |
Begara-Morales, Juan Carlos Sánchez-Calvo, Beatriz Chaki, Mounira Mata-Pérez, Capilla Valderrama, Raquel Padilla-Serrano, María Nieves López-Jaramillo, Javier Luque-Vázquez, Francisco Corpas, Francisco Javier Barroso-Albarracín, Juan Bautista |
| author_role |
author |
| author2 |
Sánchez-Calvo, Beatriz Chaki, Mounira Mata-Pérez, Capilla Valderrama, Raquel Padilla-Serrano, María Nieves López-Jaramillo, Javier Luque-Vázquez, Francisco Corpas, Francisco Javier Barroso-Albarracín, Juan Bautista |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Glutathione reductase Monodehydroascorbate reductase Nitration Nitric oxide Peroxynitrite Reactive nitrogen species Salinity S-nitrosylation S-nitrosoglutathione. |
| topic |
Glutathione reductase Monodehydroascorbate reductase Nitration Nitric oxide Peroxynitrite Reactive nitrogen species Salinity S-nitrosylation S-nitrosoglutathione. |
| description |
This is the peer reviewed version of the following article: [Juan C. Begara-Morales, Beatriz Sánchez-Calvo, Mounira Chaki, Capilla Mata-Pérez, Raquel Valderrama, María N. Padilla, Javier López-Jaramillo, Francisco Luque, Francisco J. Corpas, Juan B. Barroso, Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation, Journal of Experimental Botany, Volume 66, Issue 19, September 2015, Pages 5983–5996, https://doi.org/10.1093/jxb/erv306.] This version of the article may be used for non-commercial purposes in accordance with Oxford Academic terms and conditions for use of self-archived versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Oxford Academic or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Oxford´s version of record on Plant and Cell Physiology online library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Oxford online library must be prohibited. |
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2015 |
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2015 2025 2025 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://academic.oup.com/jxb/article/66/19/5983/697589?login=false https://hdl.handle.net/10953/4397 |
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https://academic.oup.com/jxb/article/66/19/5983/697589?login=false https://hdl.handle.net/10953/4397 |
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Inglés |
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Inglés |
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Journal of Experimental Botany |
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Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
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openAccess |
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application/pdf |
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Oxford Academic |
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Oxford Academic |
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reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén instname:Universidad de Jaén |
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Universidad de Jaén |
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RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
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RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
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1869422847545311232 |
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Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylationBegara-Morales, Juan CarlosSánchez-Calvo, BeatrizChaki, MouniraMata-Pérez, CapillaValderrama, RaquelPadilla-Serrano, María NievesLópez-Jaramillo, JavierLuque-Vázquez, FranciscoCorpas, Francisco JavierBarroso-Albarracín, Juan BautistaGlutathione reductaseMonodehydroascorbate reductaseNitrationNitric oxidePeroxynitriteReactive nitrogen speciesSalinityS-nitrosylationS-nitrosoglutathione.This is the peer reviewed version of the following article: [Juan C. Begara-Morales, Beatriz Sánchez-Calvo, Mounira Chaki, Capilla Mata-Pérez, Raquel Valderrama, María N. Padilla, Javier López-Jaramillo, Francisco Luque, Francisco J. Corpas, Juan B. Barroso, Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation, Journal of Experimental Botany, Volume 66, Issue 19, September 2015, Pages 5983–5996, https://doi.org/10.1093/jxb/erv306.] This version of the article may be used for non-commercial purposes in accordance with Oxford Academic terms and conditions for use of self-archived versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Oxford Academic or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Oxford´s version of record on Plant and Cell Physiology online library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Oxford online library must be prohibited.The ascorbate–glutathione cycle is a metabolic pathway that detoxifies hydrogen peroxide and involves enzymatic and non-enzymatic antioxidants. Proteomic studies have shown that some enzymes in this cycle such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), and glutathione reductase (GR) are potential targets for post-translational modifications (PMTs) mediated by nitric oxide-derived molecules. Using purified recombinant pea peroxisomal MDAR and cytosolic and chloroplastic GR enzymes produced in Escherichia coli, the effects of peroxynitrite (ONOO–) and S-nitrosoglutathione (GSNO) which are known to mediate protein nitration and S-nitrosylation processes, respectively, were analysed. Although ONOO– and GSNO inhibit peroxisomal MDAR activity, chloroplastic and cytosolic GR were not affected by these molecules. Mass spectrometric analysis of the nitrated MDAR revealed that Tyr213, Try292, and Tyr345 were exclusively nitrated to 3-nitrotyrosine by ONOO–. The location of these residues in the structure of pea peroxisomal MDAR reveals that Tyr345 is found at 3.3 Å of His313 which is involved in the NADP-binding site. Site-directed mutagenesis confirmed Tyr345 as the primary site of nitration responsible for the inhibition of MDAR activity by ONOO–. These results provide new insights into the molecular regulation of MDAR which is deactivated by nitration and S-nitrosylation. However, GR was not affected by ONOO– or GSNO, suggesting the existence of a mechanism to conserve redox status by maintaining the level of reduced GSH. Under a nitro-oxidative stress induced by salinity (150mM NaCl), MDAR expression (mRNA, protein, and enzyme activity levels) was increased, probably to compensate the inhibitory effects of S-nitrosylation and nitration on the enzyme. The present data show the modulation of the antioxidative response of key enzymes in the ascorbate–glutathione cycle by nitric oxide (NO)-PTMs, thus indicating the close involvement of NO and reactive oxygen species metabolism in antioxidant defence against nitro-oxidative stress situations in plants.Oxford Academic202520252015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://academic.oup.com/jxb/article/66/19/5983/697589?login=falsehttps://hdl.handle.net/10953/4397reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésJournal of Experimental BotanyAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/43972026-06-24T12:41:07Z |
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15.81155 |