Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobac...

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Autores: Chadwick, Grayson L, Skennerton, Connor T, Laso-Pérez, Rafael, Leu, Andy O, Speth, Daan R, Yu, Hang, Morgan-Lang, Connor, Hatzenpichler, Roland, Goudeau, Danielle, Malmstrom, Rex, Brazelton, William J, Woyke, Tanja, Hallam, Steven J, Tyson, Gene W, Wegener, Gunter, Boetius, Antje, Orphan, Victoria J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/351871
Acceso en línea:http://hdl.handle.net/10261/351871
https://api.elsevier.com/content/abstract/scopus_id/85122281056
Access Level:acceso abierto
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spelling Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaeaChadwick, Grayson LSkennerton, Connor TLaso-Pérez, RafaelLeu, Andy OSpeth, Daan RYu, HangMorgan-Lang, ConnorHatzenpichler, RolandGoudeau, DanielleMalmstrom, RexBrazelton, William JWoyke, TanjaHallam, Steven JTyson, Gene WWegener, GunterBoetius, AntjeOrphan, Victoria JThe anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota, none of which have been isolated in pure culture. Here, we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features that separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor.Peer reviewedLaso-Pérez, Rafael [0000-0002-6912-7865]202420242022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/351871https://api.elsevier.com/content/abstract/scopus_id/85122281056reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPLoS biologyNoinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3518712026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
title Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
spellingShingle Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
Chadwick, Grayson L
title_short Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
title_full Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
title_fullStr Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
title_full_unstemmed Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
title_sort Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea
dc.creator.none.fl_str_mv Chadwick, Grayson L
Skennerton, Connor T
Laso-Pérez, Rafael
Leu, Andy O
Speth, Daan R
Yu, Hang
Morgan-Lang, Connor
Hatzenpichler, Roland
Goudeau, Danielle
Malmstrom, Rex
Brazelton, William J
Woyke, Tanja
Hallam, Steven J
Tyson, Gene W
Wegener, Gunter
Boetius, Antje
Orphan, Victoria J
author Chadwick, Grayson L
author_facet Chadwick, Grayson L
Skennerton, Connor T
Laso-Pérez, Rafael
Leu, Andy O
Speth, Daan R
Yu, Hang
Morgan-Lang, Connor
Hatzenpichler, Roland
Goudeau, Danielle
Malmstrom, Rex
Brazelton, William J
Woyke, Tanja
Hallam, Steven J
Tyson, Gene W
Wegener, Gunter
Boetius, Antje
Orphan, Victoria J
author_role author
author2 Skennerton, Connor T
Laso-Pérez, Rafael
Leu, Andy O
Speth, Daan R
Yu, Hang
Morgan-Lang, Connor
Hatzenpichler, Roland
Goudeau, Danielle
Malmstrom, Rex
Brazelton, William J
Woyke, Tanja
Hallam, Steven J
Tyson, Gene W
Wegener, Gunter
Boetius, Antje
Orphan, Victoria J
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Laso-Pérez, Rafael [0000-0002-6912-7865]
description The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota, none of which have been isolated in pure culture. Here, we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features that separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/351871
https://api.elsevier.com/content/abstract/scopus_id/85122281056
url http://hdl.handle.net/10261/351871
https://api.elsevier.com/content/abstract/scopus_id/85122281056
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS biology
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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