Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant

[EN] Phosphate limitation in Streptomyces and in other bacteria triggers expression changes of a large number of genes. This response is mediated by the two-component PhoR-PhoP system. A Streptomyces coelicolor ΔphoP mutant (lacking phoP) has been obtained by gene replacement. A genome-wide analysis...

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Detalhes bibliográficos
Autores: Rodríguez García, Antonio, Barreiro Méndez, Carlos, Santos Beneit, Fernando, Sola Landa, Alberto, Martín Martín, Juan Francisco
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/17877
Acesso em linha:https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200600883
https://hdl.handle.net/10612/17877
Access Level:acceso abierto
Palavra-chave:Biología
Biotecnología
Phosphate regulation
PhoR-PhoP
Streptomyces
Microarray
Transcriptomics
Proteomics
ΔphoP mutant
2414.02 Fisiología Bacteriana
2415.01 Biología Molecular de Microorganismos
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spelling Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutantRodríguez García, AntonioBarreiro Méndez, CarlosSantos Beneit, FernandoSola Landa, AlbertoMartín Martín, Juan FranciscoBiologíaBiotecnologíaPhosphate regulationPhoR-PhoPStreptomycesMicroarrayTranscriptomicsProteomicsΔphoP mutant2414.02 Fisiología Bacteriana2415.01 Biología Molecular de Microorganismos[EN] Phosphate limitation in Streptomyces and in other bacteria triggers expression changes of a large number of genes. This response is mediated by the two-component PhoR-PhoP system. A Streptomyces coelicolor ΔphoP mutant (lacking phoP) has been obtained by gene replacement. A genome-wide analysis of the primary response to phosphate limitation using transcriptomic and proteomic studies has been made in the parental S. coelicolor M145 and in the ΔphoP mutant strains. Statistical analysis of the contrasts between the four sets of data generated (two strains under two phosphate conditions) allowed the classification of all genes into 12 types of profiles. The primary response to phosphate limitation involves upregulation of genes encoding scavenging enzymes needed to obtain phosphate from different phosphorylated organic compounds and overexpression of the high-affinity phosphate transport system pstSCAB. Clear interactions have been found between phosphate metabolism and expression of nitrogen-regulated genes and between phosphate and nitrate respiration genes. PhoP-dependent repressions of antibiotic biosynthesis and of the morphological differentiation genes correlated with the observed ΔphoP mutant phenotype. Bioinformatic analysis of the presence of PHO boxes (PhoP-binding sequences) in the upstream regions of PhoP-controlled genes were validated by binding of PhoP, as shown by electrophoretic mobility shift assaysSIThis work was supported by grants of the CICYT (Madrid,Spain) to J. F. M. (BIO2003-01489 and GEN2003-20245-C09-01) and of the European Union (Project ActinoGEN OJ 2003/C164). A. Rodríguez was supported by a Torres Quevedo contract (PTQ2002-0468), and F. Santos received a FPI fellowship from the Ministry of Education (Madrid, Spain). We thank J. Merino, B. Martín, A. Casenave, and B. Aguado for excellent technical assistance, and V. Mersinias, O. Domínguez, L. Lombardía, and J.C. Oliveros for helpful discussion of microarray proceduresWileyMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200600883https://hdl.handle.net/10612/17877reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MICYT//GEN2003-20245-C09-01-NACinfo:eu-repo/grantAgreement/MICYT//BIO2003-01489info:eu-repo/grantAgreement/EU//GEN-OJ-2003-C164info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/178772026-06-24T12:43:27Z
dc.title.none.fl_str_mv Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
title Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
spellingShingle Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
Rodríguez García, Antonio
Biología
Biotecnología
Phosphate regulation
PhoR-PhoP
Streptomyces
Microarray
Transcriptomics
Proteomics
ΔphoP mutant
2414.02 Fisiología Bacteriana
2415.01 Biología Molecular de Microorganismos
title_short Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
title_full Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
title_fullStr Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
title_full_unstemmed Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
title_sort Genome‐wide transcriptomic and proteomic analysis of the primary response to phosphate limitation in Streptomyces coelicolor M145 and in a ΔphoP mutant
dc.creator.none.fl_str_mv Rodríguez García, Antonio
Barreiro Méndez, Carlos
Santos Beneit, Fernando
Sola Landa, Alberto
Martín Martín, Juan Francisco
author Rodríguez García, Antonio
author_facet Rodríguez García, Antonio
Barreiro Méndez, Carlos
Santos Beneit, Fernando
Sola Landa, Alberto
Martín Martín, Juan Francisco
author_role author
author2 Barreiro Méndez, Carlos
Santos Beneit, Fernando
Sola Landa, Alberto
Martín Martín, Juan Francisco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Biotecnología
Phosphate regulation
PhoR-PhoP
Streptomyces
Microarray
Transcriptomics
Proteomics
ΔphoP mutant
2414.02 Fisiología Bacteriana
2415.01 Biología Molecular de Microorganismos
topic Biología
Biotecnología
Phosphate regulation
PhoR-PhoP
Streptomyces
Microarray
Transcriptomics
Proteomics
ΔphoP mutant
2414.02 Fisiología Bacteriana
2415.01 Biología Molecular de Microorganismos
description [EN] Phosphate limitation in Streptomyces and in other bacteria triggers expression changes of a large number of genes. This response is mediated by the two-component PhoR-PhoP system. A Streptomyces coelicolor ΔphoP mutant (lacking phoP) has been obtained by gene replacement. A genome-wide analysis of the primary response to phosphate limitation using transcriptomic and proteomic studies has been made in the parental S. coelicolor M145 and in the ΔphoP mutant strains. Statistical analysis of the contrasts between the four sets of data generated (two strains under two phosphate conditions) allowed the classification of all genes into 12 types of profiles. The primary response to phosphate limitation involves upregulation of genes encoding scavenging enzymes needed to obtain phosphate from different phosphorylated organic compounds and overexpression of the high-affinity phosphate transport system pstSCAB. Clear interactions have been found between phosphate metabolism and expression of nitrogen-regulated genes and between phosphate and nitrate respiration genes. PhoP-dependent repressions of antibiotic biosynthesis and of the morphological differentiation genes correlated with the observed ΔphoP mutant phenotype. Bioinformatic analysis of the presence of PHO boxes (PhoP-binding sequences) in the upstream regions of PhoP-controlled genes were validated by binding of PhoP, as shown by electrophoretic mobility shift assays
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200600883
https://hdl.handle.net/10612/17877
url https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200600883
https://hdl.handle.net/10612/17877
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICYT//GEN2003-20245-C09-01-NAC
info:eu-repo/grantAgreement/MICYT//BIO2003-01489
info:eu-repo/grantAgreement/EU//GEN-OJ-2003-C164
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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