Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells

Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may inter...

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Autores: Sebastián Muñoz, David, Guitart, Maria, García Martínez, Celia, Mauvezin, Caroline, Orellana Gavaldà, Josep Maria, Serra i Cucurull, Dolors, Gómez Foix, Anna Maria, García Hegardt, Fausto, Asins Muñoz, Guillermina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/164997
Acceso en línea:https://hdl.handle.net/2445/164997
Access Level:acceso abierto
Palabra clave:Metabolisme
Mitocondris
Antígens
Àcids grassos
Citologia
Reacció d'oxidació-reducció
Cèl·lules musculars
Metabolisme dels lípids
Ratolins (Animals de laboratori)
Metabolism
Mitochondria
Antigens
Fatty acids
Cytology
Oxidation-reduction reaction
Muscle cells
Lipid metabolism
Mice (Laboratory animals)
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spelling Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cellsSebastián Muñoz, DavidGuitart, MariaGarcía Martínez, CeliaMauvezin, CarolineOrellana Gavaldà, Josep MariaSerra i Cucurull, DolorsGómez Foix, Anna MariaGarcía Hegardt, FaustoAsins Muñoz, GuillerminaMetabolismeMitocondrisAntígensÀcids grassosCitologiaReacció d'oxidació-reduccióCèl·lules muscularsMetabolisme dels lípidsRatolins (Animals de laboratori)MetabolismMitochondriaAntigensFatty acidsCytologyOxidation-reduction reactionMuscle cellsLipid metabolismMice (Laboratory animals)Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may interact with CPT1 and contribute to fatty acid import into mitochondria in muscle. Here, we demonstrate that another membrane-bound fatty acid binding protein, fatty acid transport protein 1 (FATP1), collaborates with CPT1 for fatty acid import into mitochondria. Overexpression of FATP1 using adenovirus in L6E9 myotubes increased both fatty acid oxidation and palmitate esterification into triacylglycerides. Moreover, immunocytochemistry assays in transfected L6E9 myotubes showed that FATP1 was present in mitochondria and coimmunoprecipitated with CPT1 in L6E9 myotubes and rat skeletal muscle in vivo. The cooverexpression of FATP1 and CPT1 also enhanced mitochondrial fatty acid oxidation, similar to the cooverexpression of FAT/CD36 and CPT1. However, etomoxir, an irreversible inhibitor of CPT1, blocked all these effects. These data reveal that FATP1, like FAT/CD36, is associated with mitochondria and has a role in mitochondrial oxidation of fatty acids.American Society for Biochemistry and Molecular Biology2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/164997Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1194/jlr.M800535-JLR200Journal of Lipid Research, 2009, vol. 50, num. 9, p. 1789-1799https://doi.org/10.1194/jlr.M800535-JLR200(c) American Society for Biochemistry and Molecular Biology, 2009info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1649972026-05-27T06:46:51Z
dc.title.none.fl_str_mv Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
title Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
spellingShingle Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
Sebastián Muñoz, David
Metabolisme
Mitocondris
Antígens
Àcids grassos
Citologia
Reacció d'oxidació-reducció
Cèl·lules musculars
Metabolisme dels lípids
Ratolins (Animals de laboratori)
Metabolism
Mitochondria
Antigens
Fatty acids
Cytology
Oxidation-reduction reaction
Muscle cells
Lipid metabolism
Mice (Laboratory animals)
title_short Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
title_full Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
title_fullStr Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
title_full_unstemmed Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
title_sort Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
dc.creator.none.fl_str_mv Sebastián Muñoz, David
Guitart, Maria
García Martínez, Celia
Mauvezin, Caroline
Orellana Gavaldà, Josep Maria
Serra i Cucurull, Dolors
Gómez Foix, Anna Maria
García Hegardt, Fausto
Asins Muñoz, Guillermina
author Sebastián Muñoz, David
author_facet Sebastián Muñoz, David
Guitart, Maria
García Martínez, Celia
Mauvezin, Caroline
Orellana Gavaldà, Josep Maria
Serra i Cucurull, Dolors
Gómez Foix, Anna Maria
García Hegardt, Fausto
Asins Muñoz, Guillermina
author_role author
author2 Guitart, Maria
García Martínez, Celia
Mauvezin, Caroline
Orellana Gavaldà, Josep Maria
Serra i Cucurull, Dolors
Gómez Foix, Anna Maria
García Hegardt, Fausto
Asins Muñoz, Guillermina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Metabolisme
Mitocondris
Antígens
Àcids grassos
Citologia
Reacció d'oxidació-reducció
Cèl·lules musculars
Metabolisme dels lípids
Ratolins (Animals de laboratori)
Metabolism
Mitochondria
Antigens
Fatty acids
Cytology
Oxidation-reduction reaction
Muscle cells
Lipid metabolism
Mice (Laboratory animals)
topic Metabolisme
Mitocondris
Antígens
Àcids grassos
Citologia
Reacció d'oxidació-reducció
Cèl·lules musculars
Metabolisme dels lípids
Ratolins (Animals de laboratori)
Metabolism
Mitochondria
Antigens
Fatty acids
Cytology
Oxidation-reduction reaction
Muscle cells
Lipid metabolism
Mice (Laboratory animals)
description Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may interact with CPT1 and contribute to fatty acid import into mitochondria in muscle. Here, we demonstrate that another membrane-bound fatty acid binding protein, fatty acid transport protein 1 (FATP1), collaborates with CPT1 for fatty acid import into mitochondria. Overexpression of FATP1 using adenovirus in L6E9 myotubes increased both fatty acid oxidation and palmitate esterification into triacylglycerides. Moreover, immunocytochemistry assays in transfected L6E9 myotubes showed that FATP1 was present in mitochondria and coimmunoprecipitated with CPT1 in L6E9 myotubes and rat skeletal muscle in vivo. The cooverexpression of FATP1 and CPT1 also enhanced mitochondrial fatty acid oxidation, similar to the cooverexpression of FAT/CD36 and CPT1. However, etomoxir, an irreversible inhibitor of CPT1, blocked all these effects. These data reveal that FATP1, like FAT/CD36, is associated with mitochondria and has a role in mitochondrial oxidation of fatty acids.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/164997
url https://hdl.handle.net/2445/164997
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1194/jlr.M800535-JLR200
Journal of Lipid Research, 2009, vol. 50, num. 9, p. 1789-1799
https://doi.org/10.1194/jlr.M800535-JLR200
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 2009
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 2009
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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