qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals

[Background] The transcriptional response of adult zebra mussels (Dreissena polymorpha) to heavy metals (mercury, copper, and cadmium) was analyzed by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to study the coordinated regulation of different metal-, oxidative stress- and xenobiotic...

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Autores: Jaumot, Joaquim, Navarro, Anna, Faria, Melissa, Barata Martí, Carlos, Tauler, Romà, Piña, Benjamín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/125804
Acceso en línea:http://hdl.handle.net/10261/125804
Access Level:acceso abierto
Palabra clave:qRT-PCR
Chemometric analysis
Dreissena polymorpha
Metal exposure
Gene networks
Oxidative stress pathways
Transcriptional regulation
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repository_id_str
spelling qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metalsJaumot, JoaquimNavarro, AnnaFaria, MelissaBarata Martí, CarlosTauler, RomàPiña, BenjamínqRT-PCRChemometric analysisDreissena polymorphaMetal exposureGene networksOxidative stress pathwaysTranscriptional regulation[Background] The transcriptional response of adult zebra mussels (Dreissena polymorpha) to heavy metals (mercury, copper, and cadmium) was analyzed by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to study the coordinated regulation of different metal-, oxidative stress- and xenobiotic defence-related genes in gills and digestive gland. Regulatory network analyses allowed the comparison of this response between different species and taxa.[Results] Chemometric analyses allowed identifying the effects of these metals clearly separating control and treated samples of both tissues. Interactions between the different genes, either in the same or between both tissues, were analysed to identify correlations and to propose stress-related genes’ regulatory networks. These networks were finally compared with existing data from human, mouse, zebrafish, Drosophila and the roundworm to evaluate their mechanistically-known response to metals (and to stressors in general) with the correlations observed in the still poorly-known, invasive zebra mussel.[Conclusions] Our analyses found a general conservation of regulation genes and of their interactions among the different considered species, and may serve as a guide to extrapolate regulatory data from model species to lesser-known environmentally (or medically) relevant species.This work has been supported by the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement n. 320737. Recognition from the Catalan government (grant 2014SGR1106) is acknowledged. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). JJ acknowledges a CSIC JAE-Doc contract cofounded by the FSE.Peer reviewedBioMed CentralEuropean CommissionEuropean Research CouncilGeneralitat de CatalunyaCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/125804reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/320737http://dx.doi.org/10.1186/s12864-015-1567-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1258042026-05-22T06:33:51Z
dc.title.none.fl_str_mv qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
title qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
spellingShingle qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
Jaumot, Joaquim
qRT-PCR
Chemometric analysis
Dreissena polymorpha
Metal exposure
Gene networks
Oxidative stress pathways
Transcriptional regulation
title_short qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
title_full qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
title_fullStr qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
title_full_unstemmed qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
title_sort qRT-PCR evaluation of the transcriptional response of zebra mussel to heavy metals
dc.creator.none.fl_str_mv Jaumot, Joaquim
Navarro, Anna
Faria, Melissa
Barata Martí, Carlos
Tauler, Romà
Piña, Benjamín
author Jaumot, Joaquim
author_facet Jaumot, Joaquim
Navarro, Anna
Faria, Melissa
Barata Martí, Carlos
Tauler, Romà
Piña, Benjamín
author_role author
author2 Navarro, Anna
Faria, Melissa
Barata Martí, Carlos
Tauler, Romà
Piña, Benjamín
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
European Research Council
Generalitat de Catalunya
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv qRT-PCR
Chemometric analysis
Dreissena polymorpha
Metal exposure
Gene networks
Oxidative stress pathways
Transcriptional regulation
topic qRT-PCR
Chemometric analysis
Dreissena polymorpha
Metal exposure
Gene networks
Oxidative stress pathways
Transcriptional regulation
description [Background] The transcriptional response of adult zebra mussels (Dreissena polymorpha) to heavy metals (mercury, copper, and cadmium) was analyzed by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to study the coordinated regulation of different metal-, oxidative stress- and xenobiotic defence-related genes in gills and digestive gland. Regulatory network analyses allowed the comparison of this response between different species and taxa.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/125804
url http://hdl.handle.net/10261/125804
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/320737
http://dx.doi.org/10.1186/s12864-015-1567-4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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