Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies

Trifloxystrobin is one of the main active principles belonging to the strobilurin family of crop protection compounds. In this article, the synthesis of a battery of regioisomeric functionalized derivatives of trifloxystrobin is described. The same aliphatic linear carboxylated chain was introduced...

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Autores: López Moreno, Rosario, Mercader Badia, Josep Vicent, Agulló, Consuelo, Abad Somovilla, Antonio, Abad Fuentes, Antonio
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/110767
Acceso en línea:http://hdl.handle.net/10261/110767
Access Level:acceso abierto
Palabra clave:Active ester
Regioisomeric haptens
Fungicide
Strobilurin
Derivatization site
Immune response
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spelling Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodiesLópez Moreno, RosarioMercader Badia, Josep VicentAgulló, ConsueloAbad Somovilla, AntonioAbad Fuentes, AntonioActive esterRegioisomeric haptensFungicideStrobilurinDerivatization siteImmune responseTrifloxystrobin is one of the main active principles belonging to the strobilurin family of crop protection compounds. In this article, the synthesis of a battery of regioisomeric functionalized derivatives of trifloxystrobin is described. The same aliphatic linear carboxylated chain was introduced as spacer arm in all of the synthesized haptens, but it was located at different positions of the parent molecule. N,N′-Disuccinimidyl carbonate was employed for hapten activation, so the resulting N-hydroxysuccinimyl ester could be readily purified and efficiently coupled to proteins. After immunization and hybridoma generation, a collection of 20 mouse monoclonal antibodies from different immunizing haptens was obtained. The analytical performance of these immunoreagents was evaluated in terms of affinity and selectivity with the aim to develop rapid and practical immunochemical procedures for trifloxystrobin determination. © 2013 Elsevier Ltd. All rights reserved.This work was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN) (AGL2006-12750-C02-01/02/ALI and AGL2009-12940-C02-01/02/ALI) and cofinanced by FEDER funds. R.L.-M. was hired by MICINN under a predoctoral FPI grant associated to the above project. J.V.M. was hired by CSIC with a postdoctoral contract under the Ramón y Cajal program, cofinanced by MICINN and by the European Social Fund (ESF).Peer ReviewedElsevier2015201520142015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/110767reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1107672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
title Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
spellingShingle Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
López Moreno, Rosario
Active ester
Regioisomeric haptens
Fungicide
Strobilurin
Derivatization site
Immune response
title_short Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
title_full Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
title_fullStr Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
title_full_unstemmed Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
title_sort Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies
dc.creator.none.fl_str_mv López Moreno, Rosario
Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author López Moreno, Rosario
author_facet López Moreno, Rosario
Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author_role author
author2 Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Active ester
Regioisomeric haptens
Fungicide
Strobilurin
Derivatization site
Immune response
topic Active ester
Regioisomeric haptens
Fungicide
Strobilurin
Derivatization site
Immune response
description Trifloxystrobin is one of the main active principles belonging to the strobilurin family of crop protection compounds. In this article, the synthesis of a battery of regioisomeric functionalized derivatives of trifloxystrobin is described. The same aliphatic linear carboxylated chain was introduced as spacer arm in all of the synthesized haptens, but it was located at different positions of the parent molecule. N,N′-Disuccinimidyl carbonate was employed for hapten activation, so the resulting N-hydroxysuccinimyl ester could be readily purified and efficiently coupled to proteins. After immunization and hybridoma generation, a collection of 20 mouse monoclonal antibodies from different immunizing haptens was obtained. The analytical performance of these immunoreagents was evaluated in terms of affinity and selectivity with the aim to develop rapid and practical immunochemical procedures for trifloxystrobin determination. © 2013 Elsevier Ltd. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/110767
url http://hdl.handle.net/10261/110767
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422794906796032
score 15,811543

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