Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects

Evaluate: the in vivo and in vitro responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients sensitized to olive tree pollen (OL) confirmed by nasal allergen provocation test (NAPT). Methods: Twelve subjects with AR, 12 with LAR, and 12 subjects as control group (CG)...

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Autores: Campo, Paloma, Villalba Díaz, María Teresa, Barrionuevo, Esther, Rondón, Carmen, Galindo, Luisa, Rodríguez, María José, López Rodríguez, Juan Carlos, Torres, María José, Blanca, Miguel, Mayorga, Cristobalina
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/34857
Acceso en línea:https://hdl.handle.net/20.500.14352/34857
Access Level:acceso abierto
Palabra clave:577.1
616-056.3
Basophil activation test
local allergic rhinitis
nasal allergen provocation test
purified allergen
Alergología
Bioquímica (Biología)
3207.01 Alergias
2302 Bioquímica
id ES_e6bc763247b93e484980d42e2b093aa8
oai_identifier_str oai:docta.ucm.es:20.500.14352/34857
network_acronym_str ES
network_name_str España
repository_id_str
spelling Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjectsCampo, PalomaVillalba Díaz, María TeresaBarrionuevo, EstherRondón, CarmenGalindo, LuisaRodríguez, María JoséLópez Rodríguez, Juan CarlosTorres, María JoséBlanca, MiguelMayorga, Cristobalina577.1616-056.3Basophil activation testlocal allergic rhinitisnasal allergen provocation testpurified allergenAlergologíaBioquímica (Biología)3207.01 Alergias2302 BioquímicaEvaluate: the in vivo and in vitro responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients sensitized to olive tree pollen (OL) confirmed by nasal allergen provocation test (NAPT). Methods: Twelve subjects with AR, 12 with LAR, and 12 subjects as control group (CG) were selected. Skin testing and NAPT with nOle e 1 were performed. ECP and tryptase were measured in nasal lavages before and after NAPT. Serum IgE to OL allergens were measured by ELISA. Basophil activation tests (BAT) with OL and nOle e 1 and dendritic cell maturation/proliferation studies were carried out. Results: All AR (12/12) and 10/12 (83%) of LAR had a +NAPT to nOle e 1. ECP levels in nasal lavages were significantly increased after NAPT in both AR and LAR compared with CG at 15 minutes (p<0.05). Serum IgE was positive only in AR. All AR had +BAT responses to OL and 10/12 to nOle e 1 (83%); 8/12 LAR (66.6%) had a +BAT with OL and 4/12 (33%) to nOle e 1, with only one subject of the control group with a +BAT to both OL and nOle e 1 (8%). Dendritic cell proliferation to nOle e 1 was increased in AR compared to LAR and CG (p=0.019 and p=0.001 respectively).Conclusion: Both AR and LAR had a similar in vivo response to nOle e 1 with release of inflammatory mediators. Specific basophil activation with OL and nOle e 1 was observed in LAR confirming previous data obtained with dust mites.BioMed CentralUniversidad Complutense de Madrid20152015-01-0120152015-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/34857reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/348572026-06-02T12:44:21Z
dc.title.none.fl_str_mv Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
title Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
spellingShingle Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
Campo, Paloma
577.1
616-056.3
Basophil activation test
local allergic rhinitis
nasal allergen provocation test
purified allergen
Alergología
Bioquímica (Biología)
3207.01 Alergias
2302 Bioquímica
title_short Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
title_full Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
title_fullStr Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
title_full_unstemmed Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
title_sort Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
dc.creator.none.fl_str_mv Campo, Paloma
Villalba Díaz, María Teresa
Barrionuevo, Esther
Rondón, Carmen
Galindo, Luisa
Rodríguez, María José
López Rodríguez, Juan Carlos
Torres, María José
Blanca, Miguel
Mayorga, Cristobalina
author Campo, Paloma
author_facet Campo, Paloma
Villalba Díaz, María Teresa
Barrionuevo, Esther
Rondón, Carmen
Galindo, Luisa
Rodríguez, María José
López Rodríguez, Juan Carlos
Torres, María José
Blanca, Miguel
Mayorga, Cristobalina
author_role author
author2 Villalba Díaz, María Teresa
Barrionuevo, Esther
Rondón, Carmen
Galindo, Luisa
Rodríguez, María José
López Rodríguez, Juan Carlos
Torres, María José
Blanca, Miguel
Mayorga, Cristobalina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
616-056.3
Basophil activation test
local allergic rhinitis
nasal allergen provocation test
purified allergen
Alergología
Bioquímica (Biología)
3207.01 Alergias
2302 Bioquímica
topic 577.1
616-056.3
Basophil activation test
local allergic rhinitis
nasal allergen provocation test
purified allergen
Alergología
Bioquímica (Biología)
3207.01 Alergias
2302 Bioquímica
description Evaluate: the in vivo and in vitro responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients sensitized to olive tree pollen (OL) confirmed by nasal allergen provocation test (NAPT). Methods: Twelve subjects with AR, 12 with LAR, and 12 subjects as control group (CG) were selected. Skin testing and NAPT with nOle e 1 were performed. ECP and tryptase were measured in nasal lavages before and after NAPT. Serum IgE to OL allergens were measured by ELISA. Basophil activation tests (BAT) with OL and nOle e 1 and dendritic cell maturation/proliferation studies were carried out. Results: All AR (12/12) and 10/12 (83%) of LAR had a +NAPT to nOle e 1. ECP levels in nasal lavages were significantly increased after NAPT in both AR and LAR compared with CG at 15 minutes (p<0.05). Serum IgE was positive only in AR. All AR had +BAT responses to OL and 10/12 to nOle e 1 (83%); 8/12 LAR (66.6%) had a +BAT with OL and 4/12 (33%) to nOle e 1, with only one subject of the control group with a +BAT to both OL and nOle e 1 (8%). Dendritic cell proliferation to nOle e 1 was increased in AR compared to LAR and CG (p=0.019 and p=0.001 respectively).Conclusion: Both AR and LAR had a similar in vivo response to nOle e 1 with release of inflammatory mediators. Specific basophil activation with OL and nOle e 1 was observed in LAR confirming previous data obtained with dust mites.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/34857
url https://hdl.handle.net/20.500.14352/34857
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422792634531840
score 15,301603

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