Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)

https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591

Detalhes bibliográficos
Autores: González Carracedo, Mario Andrés, Tejera Pérez, Hugo, Hernández Ferrer, Mariano, Jiménez Arias, David, Pérez Pérez, José Antonio
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad de La Laguna (ULL)
Repositorio:RIULL. Repositorio Institucional de la Universidad de La Laguna
OAI Identifier:oai:riull.ull.es:915/41771
Acesso em linha:http://riull.ull.es/xmlui/handle/915/41771
Access Level:acceso abierto
Palavra-chave:cultivars
iPBS
LTR-retrotransposon
microsatellite
Musa
SSR
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oai_identifier_str oai:riull.ull.es:915/41771
network_acronym_str ES
network_name_str España
repository_id_str
spelling Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)González Carracedo, Mario AndrésTejera Pérez, HugoHernández Ferrer, MarianoJiménez Arias, DavidPérez Pérez, José AntoniocultivarsiPBSLTR-retrotransposonmicrosatelliteMusaSSRhttps://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591Banana cultivars of agronomic interest have been genetically characterized using two different molecular markers. On the one hand, a panel of 14 trinucleotide single sequence repeats (SSRs or microsatellites) was optimized for homogeneous PCR conditions. It was tested with 50 individuals from seven cultivars, yielding 76 alleles and 5.4 ± 1.8 alleles per locus, while the presence of cultivar-exclusive alleles allowed the discrimination of all cultivars. On the other hand, a retrotransposon-based marker system named inter-primer binding site (iPBS) was implemented for the first time in the Musa genus. A total of 120 bands were detected in eight different Musa cultivars, from which 65.8% were polymorphic and 23.3% were cultivar exclusive. Both techniques allowed a cut-off identification of all cultivars studied, but overall, iPBS analysis was a more straightforward and economical choice. Despite the fact that we were unable to distinguish local banana varieties belonging to the same cultivar, new cultivar-specific molecular markers have been developed for the Musa genus, which could be used to guide new breeding programmes and maintain high quality of Plátano de Canarias.Bioquímica, Microbiología, Biología Celular y Genética202520252021info:eu-repo/semantics/articleapplication/pdfhttp://riull.ull.es/xmlui/handle/915/41771reponame:RIULL. Repositorio Institucional de la Universidad de La Lagunainstname:Universidad de La Laguna (ULL)InglésPlant Breed. 2021; 140:968–980Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ESoai:riull.ull.es:915/417712026-06-22T13:13:57Z
dc.title.none.fl_str_mv Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
title Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
spellingShingle Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
González Carracedo, Mario Andrés
cultivars
iPBS
LTR-retrotransposon
microsatellite
Musa
SSR
title_short Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
title_full Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
title_fullStr Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
title_full_unstemmed Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
title_sort Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
dc.creator.none.fl_str_mv González Carracedo, Mario Andrés
Tejera Pérez, Hugo
Hernández Ferrer, Mariano
Jiménez Arias, David
Pérez Pérez, José Antonio
author González Carracedo, Mario Andrés
author_facet González Carracedo, Mario Andrés
Tejera Pérez, Hugo
Hernández Ferrer, Mariano
Jiménez Arias, David
Pérez Pérez, José Antonio
author_role author
author2 Tejera Pérez, Hugo
Hernández Ferrer, Mariano
Jiménez Arias, David
Pérez Pérez, José Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica, Microbiología, Biología Celular y Genética
dc.subject.none.fl_str_mv cultivars
iPBS
LTR-retrotransposon
microsatellite
Musa
SSR
topic cultivars
iPBS
LTR-retrotransposon
microsatellite
Musa
SSR
description https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591
publishDate 2021
dc.date.none.fl_str_mv 2021
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://riull.ull.es/xmlui/handle/915/41771
url http://riull.ull.es/xmlui/handle/915/41771
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plant Breed. 2021; 140:968–980
dc.rights.none.fl_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
rights_invalid_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIULL. Repositorio Institucional de la Universidad de La Laguna
instname:Universidad de La Laguna (ULL)
instname_str Universidad de La Laguna (ULL)
reponame_str RIULL. Repositorio Institucional de la Universidad de La Laguna
collection RIULL. Repositorio Institucional de la Universidad de La Laguna
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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