New bacterial strains for ibuprofen biodegradation: Drug removal, transformation, and potential catabolic genes
Ibuprofen (IBU) is a significant contaminant frequently found in wastewatertreatment plants due to its widespread use and limited removal during treat-ment processes. This leads to its discharge into the environment, causingconsiderable environmental concerns. The use of microorganisms hasrecently b...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/167443 |
| Acceso en línea: | https://hdl.handle.net/11441/167443 https://doi.org/10.1111/1758-2229.13320 |
| Access Level: | acceso abierto |
| Sumario: | Ibuprofen (IBU) is a significant contaminant frequently found in wastewatertreatment plants due to its widespread use and limited removal during treat-ment processes. This leads to its discharge into the environment, causingconsiderable environmental concerns. The use of microorganisms hasrecently been recognized as a sustainable method for mitigating IBU con-tamination in wastewater. In this study, new bacteria capable of growing ina solid medium with IBU as the only carbon source and removing IBU froma liquid medium were isolated from environmental samples, including soil,marine, mine, and olive mill wastewater. Four bacterial strains, namely Kleb-siella pneumoniae TIBU2.1, Klebsiella variicola LOIBU1.1, Pseudomonasaeruginosa LOIBU1.2, and Mycolicibacterium aubagnense HPB1.1, wereidentified through 16S rRNA gene sequencing. These strains demonstratedsignificant IBU removal efficiencies, ranging from 60 to 100% within14 days, starting from an initial IBU concentration of 5 mg per litre. Thesebacteria have not been previously reported in the literature as IBUdegraders, making this work a valuable contribution to further studies in thefield of bioremediation in environments contaminated by IBU. Based on theIBU removal results, the most promising bacteria, K. pneumoniae TIBU2.1and M. aubagnense HPB1.1, were selected for an in silico analysis to iden-tify genes potentially involved in IBU biodegradation. Interestingly, in thetests with TIBU2.1, a peak of IBU transformation product(s) was detectedby high-performance liquid chromatography, while in the tests with HPB1.1,it was not detected. The emerging peak was analysed by liquidchromatography–mass spectrometry, indicating the presence of possibleconjugates between intermediates of IBU biodegradation. The proteinsencoded on their whole-genome sequences were aligned with proteinsinvolved in an IBU-degrading pathway reported in bacteria with respectivecatabolic genes. The analysis indicated that strain HPB1.1 possessesgenes encoding proteins similar to most enzymes reported associated withthe IBU metabolic pathways used as reference bacteria, while strainTIBU2.1 has genes encoding proteins similar to enzymes involved in boththe upper and the lower part of that pathway. Notably, in the tests with thestrain having more candidate genes encoding IBU-catabolic enzymes, noIBU transformation products were detected, while in the tests with the strainhaving fewer of these genes, detection occurred.Received: 4 May 2024 Accepted: 13 July 2024DOI: 10.1111/1758-2229.13320ENVIRONMENTAL MICROBIOLOGY REPORTSThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,provided the original work is properly cited.© 2024 The Author(s). Environmental Microbiology Reports published by John Wiley & Sons Ltd.Environmental Microbiology Reports. 2024;16:e13320. wileyonlinelibrary.com/journal/emi4 1 of 23https://doi.org/10.1111/1758-2229.13320 |
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