Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA

Candida albicans is a human pathogen of significant clinical relevance. This pathogen is resistant to different drugs, and most clinical antifungals are not effective against the prevention and treatment of C. albicans infections. As with other microorganisms, it can produce biofilms that serve as a...

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Autores: Gómez Casanova, Natalia|||0000-0002-6983-1895, Lozano de la Cruz, Tania, Soliveri de Carranza, Juan|||0000-0001-8200-0132, Gómez Ramírez, Rafael|||0000-0001-6448-2414, Ortega Lopez, Paula|||0000-0003-0377-5429, Copa Patiño, José Luis|||0000-0002-2162-3907, Heredero Bermejo, Irene|||0000-0001-6984-1965
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/59767
Acceso en línea:http://hdl.handle.net/10017/59767
https://dx.doi.org/10.3390/ jof7070574
Access Level:acceso abierto
Palabra clave:Candida albicans
carbosilane dendron
silver nitrate
EDTA
biofilm
synergy
Química
Chemistry
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spelling Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTAGómez Casanova, Natalia|||0000-0002-6983-1895Lozano de la Cruz, TaniaSoliveri de Carranza, Juan|||0000-0001-8200-0132Gómez Ramírez, Rafael|||0000-0001-6448-2414Ortega Lopez, Paula|||0000-0003-0377-5429Copa Patiño, José Luis|||0000-0002-2162-3907Heredero Bermejo, Irene|||0000-0001-6984-1965Candida albicanscarbosilane dendronsilver nitrateEDTAbiofilmsynergyQuímicaChemistryCandida albicans is a human pathogen of significant clinical relevance. This pathogen is resistant to different drugs, and most clinical antifungals are not effective against the prevention and treatment of C. albicans infections. As with other microorganisms, it can produce biofilms that serve as a barrier against antifungal agents and other substances, contributing to infection in humans and environmental tolerance of this microorganism. Thus, resistances and biofilm formation make treatment difficult. In addition, the complete eradication of biofilms in implants, catheters and other medical devices, is challenging and necessary to prevent relapses of candidemia. Therefore, it is a priority to find new molecules or combinations of compounds with anti-Candida biofilm activity. Due to the difficulty of treating and removing biofilms, the aim of this study was to evaluate the in vitro ability of different generation of cationic carbosilane dendrons derived from 4-phenylbutyric acid, ArCO(2)G(n)(SNMe3I)(m), to eradicate C. albicans biofilms. Here, we assessed the antifungal activity of the second generation dendron ArCO2G(2)(SNMe3I)(4) against C. albicans cells and established biofilms since it managed to seriously damage the membrane. In addition, the combinations of the second generation dendron with AgNO3 or EDTA eradicated the viability of biofilm cells. Alterations were observed by scanning electron microscopy and cytotoxicity was assessed on HeLa cells. Our data suggest that the dendritic compound ArCO(2)G(2)(SNMe3I)(4) could represent an alternative to control the infections caused by this pathogen.Ministerio de Economía, Industria y CompetitividadComunidad de MadridUniversidad de Alcalá20212021-07-18journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/59767https://dx.doi.org/10.3390/ jof7070574reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2017-86224-PComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703UAH Not available CCG19%2FCCS-022UAH Not available CCG20CCS-013open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/597672026-06-18T11:13:07Z
dc.title.none.fl_str_mv Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
title Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
spellingShingle Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
Gómez Casanova, Natalia|||0000-0002-6983-1895
Candida albicans
carbosilane dendron
silver nitrate
EDTA
biofilm
synergy
Química
Chemistry
title_short Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
title_full Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
title_fullStr Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
title_full_unstemmed Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
title_sort Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
dc.creator.none.fl_str_mv Gómez Casanova, Natalia|||0000-0002-6983-1895
Lozano de la Cruz, Tania
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Ortega Lopez, Paula|||0000-0003-0377-5429
Copa Patiño, José Luis|||0000-0002-2162-3907
Heredero Bermejo, Irene|||0000-0001-6984-1965
author Gómez Casanova, Natalia|||0000-0002-6983-1895
author_facet Gómez Casanova, Natalia|||0000-0002-6983-1895
Lozano de la Cruz, Tania
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Ortega Lopez, Paula|||0000-0003-0377-5429
Copa Patiño, José Luis|||0000-0002-2162-3907
Heredero Bermejo, Irene|||0000-0001-6984-1965
author_role author
author2 Lozano de la Cruz, Tania
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Ortega Lopez, Paula|||0000-0003-0377-5429
Copa Patiño, José Luis|||0000-0002-2162-3907
Heredero Bermejo, Irene|||0000-0001-6984-1965
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Candida albicans
carbosilane dendron
silver nitrate
EDTA
biofilm
synergy
Química
Chemistry
topic Candida albicans
carbosilane dendron
silver nitrate
EDTA
biofilm
synergy
Química
Chemistry
description Candida albicans is a human pathogen of significant clinical relevance. This pathogen is resistant to different drugs, and most clinical antifungals are not effective against the prevention and treatment of C. albicans infections. As with other microorganisms, it can produce biofilms that serve as a barrier against antifungal agents and other substances, contributing to infection in humans and environmental tolerance of this microorganism. Thus, resistances and biofilm formation make treatment difficult. In addition, the complete eradication of biofilms in implants, catheters and other medical devices, is challenging and necessary to prevent relapses of candidemia. Therefore, it is a priority to find new molecules or combinations of compounds with anti-Candida biofilm activity. Due to the difficulty of treating and removing biofilms, the aim of this study was to evaluate the in vitro ability of different generation of cationic carbosilane dendrons derived from 4-phenylbutyric acid, ArCO(2)G(n)(SNMe3I)(m), to eradicate C. albicans biofilms. Here, we assessed the antifungal activity of the second generation dendron ArCO2G(2)(SNMe3I)(4) against C. albicans cells and established biofilms since it managed to seriously damage the membrane. In addition, the combinations of the second generation dendron with AgNO3 or EDTA eradicated the viability of biofilm cells. Alterations were observed by scanning electron microscopy and cytotoxicity was assessed on HeLa cells. Our data suggest that the dendritic compound ArCO(2)G(2)(SNMe3I)(4) could represent an alternative to control the infections caused by this pathogen.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/59767
https://dx.doi.org/10.3390/ jof7070574
url http://hdl.handle.net/10017/59767
https://dx.doi.org/10.3390/ jof7070574
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2017-86224-P
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703
UAH Not available CCG19%2FCCS-022
UAH Not available CCG20CCS-013
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
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