Impact of the Potential m(6) A Modification Sites at the 3 &apos

[EN] We have previously reported the presence of m(6)A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m(6)A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site ((2012)AAACU(2016)) is in the loop of hpB, within the coat protein b...

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Autores: Alvarado-Marchena, Luis, Martínez-Pérez, Mireya, Úbeda, Jesús R., Pallás Benet, Vicente|||0000-0003-4954-989X, APARICIO HERRERO, FREDERIC|||0000-0002-1586-9978
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/194257
Acceso en línea:https://riunet.upv.es/handle/10251/194257
Access Level:acceso abierto
Palabra clave:N-6-methyladenosine
RNA covalent modifications
Plant alfamovirus
DRACH motif
In vivo AMV replication
3 &apos
UTR
BIOQUIMICA Y BIOLOGIA MOLECULAR
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oai_identifier_str oai:riunet.upv.es:10251/194257
network_acronym_str ES
network_name_str España
repository_id_str
spelling Impact of the Potential m(6) A Modification Sites at the 3 &aposUTR of Alfalfa Mosaic Virus RNA3 in the Viral InfectionAlvarado-Marchena, LuisMartínez-Pérez, MireyaÚbeda, Jesús R.Pallás Benet, Vicente|||0000-0003-4954-989XAPARICIO HERRERO, FREDERIC|||0000-0002-1586-9978N-6-methyladenosineRNA covalent modificationsPlant alfamovirusDRACH motifIn vivo AMV replication3 &aposUTRBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] We have previously reported the presence of m(6)A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m(6)A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site ((2012)AAACU(2016)) is in the loop of hpB, within the coat protein binding site 1 (CPB1), while the other ((1900)UGACC(1904)) is in the lower stem of hpE, a loop previously associated with AMV negative-strand RNA synthesis. In this work, we have performed in vivo experiments to assess the role of these two regions, containing the putative m(6)A-sites in the AMV cycle, by introducing compensatory point mutations to interfere with or abolish the m(6)A-tag of these sites. Our results suggest that the loop of hpB could be involved in viral replication/accumulation. Meanwhile, in the (1900)UGACC(1904) motif of the hpE, the maintenance of the adenosine residue and the lower stem hpE structure are necessary for in vivo plus-strand accumulation. These results extend our understanding of the requirements for hpE in the AMV infection cycle, indicating that both the residue identity and the base-pairing capacity in this structure are essential for viral accumulation.This research was funded by grant PID2020-115571RB-I00 to F.A and V.P., from the Spanish MCIN/AEI/10.13039/501100011033 granting agency. L.A.-M. was the recipient of a predoctoral contract from the Ministerio de Ciencia, Tecnologia y Telecomunicaciones, from Costa Rica (MICITTPINN-CON-624-2019).MDPI AGInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalAgencia Estatal de InvestigaciónMinisterio de Ciencia Tecnología y Telecomunicaciones de Costa RicaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-08-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/194257reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115571RB-I00 LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTASMinisterio de Ciencia Tecnología y Telecomunicaciones de Costa Rica https://doi.org/10.13039/501100006473 MICITT-PINN-CON-624-2019open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1942572026-06-13T07:49:27Z
dc.title.none.fl_str_mv Impact of the Potential m(6) A Modification Sites at the 3 &apos
UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title Impact of the Potential m(6) A Modification Sites at the 3 &apos
spellingShingle Impact of the Potential m(6) A Modification Sites at the 3 &apos
Alvarado-Marchena, Luis
N-6-methyladenosine
RNA covalent modifications
Plant alfamovirus
DRACH motif
In vivo AMV replication
3 &apos
UTR
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Impact of the Potential m(6) A Modification Sites at the 3 &apos
title_full Impact of the Potential m(6) A Modification Sites at the 3 &apos
title_fullStr Impact of the Potential m(6) A Modification Sites at the 3 &apos
title_full_unstemmed Impact of the Potential m(6) A Modification Sites at the 3 &apos
title_sort Impact of the Potential m(6) A Modification Sites at the 3 &apos
dc.creator.none.fl_str_mv Alvarado-Marchena, Luis
Martínez-Pérez, Mireya
Úbeda, Jesús R.
Pallás Benet, Vicente|||0000-0003-4954-989X
APARICIO HERRERO, FREDERIC|||0000-0002-1586-9978
author Alvarado-Marchena, Luis
author_facet Alvarado-Marchena, Luis
Martínez-Pérez, Mireya
Úbeda, Jesús R.
Pallás Benet, Vicente|||0000-0003-4954-989X
APARICIO HERRERO, FREDERIC|||0000-0002-1586-9978
author_role author
author2 Martínez-Pérez, Mireya
Úbeda, Jesús R.
Pallás Benet, Vicente|||0000-0003-4954-989X
APARICIO HERRERO, FREDERIC|||0000-0002-1586-9978
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Agencia Estatal de Investigación
Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv N-6-methyladenosine
RNA covalent modifications
Plant alfamovirus
DRACH motif
In vivo AMV replication
3 &apos
UTR
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic N-6-methyladenosine
RNA covalent modifications
Plant alfamovirus
DRACH motif
In vivo AMV replication
3 &apos
UTR
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] We have previously reported the presence of m(6)A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m(6)A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site ((2012)AAACU(2016)) is in the loop of hpB, within the coat protein binding site 1 (CPB1), while the other ((1900)UGACC(1904)) is in the lower stem of hpE, a loop previously associated with AMV negative-strand RNA synthesis. In this work, we have performed in vivo experiments to assess the role of these two regions, containing the putative m(6)A-sites in the AMV cycle, by introducing compensatory point mutations to interfere with or abolish the m(6)A-tag of these sites. Our results suggest that the loop of hpB could be involved in viral replication/accumulation. Meanwhile, in the (1900)UGACC(1904) motif of the hpE, the maintenance of the adenosine residue and the lower stem hpE structure are necessary for in vivo plus-strand accumulation. These results extend our understanding of the requirements for hpE in the AMV infection cycle, indicating that both the residue identity and the base-pairing capacity in this structure are essential for viral accumulation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-08-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/194257
url https://riunet.upv.es/handle/10251/194257
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115571RB-I00 LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS
Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica https://doi.org/10.13039/501100006473 MICITT-PINN-CON-624-2019
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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