EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer

Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facil...

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Detalles Bibliográficos
Autores: Roman-Canal, Berta|||0000-0002-6784-0077, Tarragona, Jordi|||0000-0002-1459-4087, Moiola, Cristian Pablo|||0000-0001-7218-0184, Gatius, Sònia, Bonnin, Sarah|||0000-0001-5159-2518, Ruiz-Miró, Maria, Sierra, José Enrique, Rufas, Maria, González, Esperanza, Porcel, José M.|||0000-0002-2734-8061, Gil-Moreno, Antonio|||0000-0003-1106-5590, Falcón-Pérez, Juan M.|||0000-0003-3133-0670, Ponomarenko, Julia|||0000-0002-1477-9444, Matías-Guiu, Xavier|||0000-0002-7201-6605, Colás Ortega, Eva|||0000-0003-0302-4828
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:226506
Acceso en línea:https://ddd.uab.cat/record/226506
https://dx.doi.org/urn:doi:10.1186/s12967-019-1954-8
Access Level:acceso abierto
Palabra clave:Colorectal cancer
Biomarkers
Diagnostic
Mirnas
Ascitic fluid
Peritoneal lavage
Liquid biopsy
Extracellular vesicles
Colon cancer
Descripción
Sumario:Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC. The online version of this article (10.1186/s12967-019-1954-8) contains supplementary material, which is available to authorized users.

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