Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone

Apoptosis plays an essential role in normal spermatogenesis, but deregulations of this biological process, which is closely associated with male infertility, have been found. Whereas calcium homeostasis is a key regulator of cell survival, sustained elevation of intracellular calcium plays a role in...

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Authors: Lozano Cordero, Graciela María, Bejarano Hernando, Ignacio, Espino Palma, Javier, González Flores, David, Ortiz Ruiz, Águeda, García Malpartida, Juan Francisco, Rodríguez Moratinos, Ana Beatriz, Pariente Llanos, José Antonio
Format: article
Publication Date:2009
Country:España
Institution:Universidad de Cantabria (UC)
Repository:UCrea Repositorio Abierto de la Universidad de Cantabria
Language:English
OAI Identifier:oai:repositorio.unican.es:10902/38792
Online Access:https://hdl.handle.net/10902/38792
Access Level:Open access
Keyword:Apoptosis
Caspases
Phosphatidylserine exposure
Spermatozoa
TUNEL
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spelling Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesteroneLozano Cordero, Graciela MaríaBejarano Hernando, IgnacioEspino Palma, JavierGonzález Flores, DavidOrtiz Ruiz, ÁguedaGarcía Malpartida, Juan FranciscoRodríguez Moratinos, Ana BeatrizPariente Llanos, José AntonioApoptosisCaspasesPhosphatidylserine exposureSpermatozoaTUNELApoptosis plays an essential role in normal spermatogenesis, but deregulations of this biological process, which is closely associated with male infertility, have been found. Whereas calcium homeostasis is a key regulator of cell survival, sustained elevation of intracellular calcium plays a role in apoptosis. The aim of this research was to determine the role of two different calcium mobilizing agents, hydrogen peroxide (H2O2) and the physiological agonist progesterone, on the apoptosis process of human ejaculated spermatozoa. Translocation of membrane phosphatidylserine was examined with an annexin V binding assay, DNA damage was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and caspase-3 activity was assessed using a fluorometric assay. After incubation of spermatozoa for 1 h with either 10 µM H2O2 or 20 µM of progesterone, there was a significant increase in both caspase-3 activity and the percentage of annexin V-positive cells. Similarly, the TUNEL results were significantly higher 1 h after incubation with either 10 µM H2O2 or 20 µM of progesterone. In fact, progesterone-treated cells showed a three-fold increase (from 17.6 to 52.9%) of TUNEL-positive cells compared to untreated cells, while H2O2-treated cells exhibited a two-fold increase (from 17.6 to 37.9%). In sum, our results suggest that spermatozoa treated with calcium mobilizing agents, such as H2O2 and progesterone, seem to undergo an apoptosis process that is dependent on caspase-3 activation.Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesteroneThe Society for Reproduction and DevelopmentUniversidad de Cantabria20092009-12-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttps://hdl.handle.net/10902/38792Journal of Reproduction and Development, 2009, 55(6), 615-621reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/387922026-06-02T12:39:31Z
dc.title.none.fl_str_mv Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
title Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
spellingShingle Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
Lozano Cordero, Graciela María
Apoptosis
Caspases
Phosphatidylserine exposure
Spermatozoa
TUNEL
title_short Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
title_full Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
title_fullStr Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
title_full_unstemmed Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
title_sort Relationship between caspase activity and apoptotic markers in human sperm in response to hydrogen peroxide and progesterone
dc.creator.none.fl_str_mv Lozano Cordero, Graciela María
Bejarano Hernando, Ignacio
Espino Palma, Javier
González Flores, David
Ortiz Ruiz, Águeda
García Malpartida, Juan Francisco
Rodríguez Moratinos, Ana Beatriz
Pariente Llanos, José Antonio
author Lozano Cordero, Graciela María
author_facet Lozano Cordero, Graciela María
Bejarano Hernando, Ignacio
Espino Palma, Javier
González Flores, David
Ortiz Ruiz, Águeda
García Malpartida, Juan Francisco
Rodríguez Moratinos, Ana Beatriz
Pariente Llanos, José Antonio
author_role author
author2 Bejarano Hernando, Ignacio
Espino Palma, Javier
González Flores, David
Ortiz Ruiz, Águeda
García Malpartida, Juan Francisco
Rodríguez Moratinos, Ana Beatriz
Pariente Llanos, José Antonio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Apoptosis
Caspases
Phosphatidylserine exposure
Spermatozoa
TUNEL
topic Apoptosis
Caspases
Phosphatidylserine exposure
Spermatozoa
TUNEL
description Apoptosis plays an essential role in normal spermatogenesis, but deregulations of this biological process, which is closely associated with male infertility, have been found. Whereas calcium homeostasis is a key regulator of cell survival, sustained elevation of intracellular calcium plays a role in apoptosis. The aim of this research was to determine the role of two different calcium mobilizing agents, hydrogen peroxide (H2O2) and the physiological agonist progesterone, on the apoptosis process of human ejaculated spermatozoa. Translocation of membrane phosphatidylserine was examined with an annexin V binding assay, DNA damage was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and caspase-3 activity was assessed using a fluorometric assay. After incubation of spermatozoa for 1 h with either 10 µM H2O2 or 20 µM of progesterone, there was a significant increase in both caspase-3 activity and the percentage of annexin V-positive cells. Similarly, the TUNEL results were significantly higher 1 h after incubation with either 10 µM H2O2 or 20 µM of progesterone. In fact, progesterone-treated cells showed a three-fold increase (from 17.6 to 52.9%) of TUNEL-positive cells compared to untreated cells, while H2O2-treated cells exhibited a two-fold increase (from 17.6 to 37.9%). In sum, our results suggest that spermatozoa treated with calcium mobilizing agents, such as H2O2 and progesterone, seem to undergo an apoptosis process that is dependent on caspase-3 activation.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10902/38792
url https://hdl.handle.net/10902/38792
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv The Society for Reproduction and Development
publisher.none.fl_str_mv The Society for Reproduction and Development
dc.source.none.fl_str_mv Journal of Reproduction and Development, 2009, 55(6), 615-621
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422732567904256
score 15,81155

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