Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism

Ripening-related firmness loss shortens considerably the storage potential of sweet cherry (Prunus avium L.), thus limiting postharvest handling, transportation, and commercialisation. The biochemical mechanisms underlying this process in cherries are not fully understood, and the mechanisms operati...

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Authors: Belge, Burcu, Goulao, Luis F., Comabella, Eva, Graell i Sarle, Jordi, Lara Ayala, Isabel
Format: article
Status:Versión aceptada para publicación
Publication Date:2017
Country:España
Institution:Universitat de Lleida (UdL)
Repository:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/59387
Online Access:https://doi.org/10.1016/j.scienta.2017.02.039
http://hdl.handle.net/10459.1/59387
Access Level:Open access
Keyword:Firmness loss
Sweet cherry
Gene expression
Prunus avium L.
Enzims
Fruita de pinyol
Enzymes
Stone fruit
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spelling Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolismBelge, BurcuGoulao, Luis F.Comabella, EvaGraell i Sarle, JordiLara Ayala, IsabelFirmness lossSweet cherryGene expressionPrunus avium L.EnzimsFruita de pinyolEnzymesStone fruitRipening-related firmness loss shortens considerably the storage potential of sweet cherry (Prunus avium L.), thus limiting postharvest handling, transportation, and commercialisation. The biochemical mechanisms underlying this process in cherries are not fully understood, and the mechanisms operating in a given fruit may be not extrapolated to a different species. Cell wall materials obtained from untreated and calcium-treated 'Celeste' sweet cherries were fractionated and analysed after cold storage, and related enzyme activities and gene expression were assessed. Calcium-treated fruit were firmer, with lower weight loss and decay incidence than the controls. The accumulation of PaβGal and PaEXP1 transcripts was strongly inhibited in cold-stored fruit, although expression levels recovered largely after three days of shelf life. Data suggest that pectin methylesterase, β-galactosidase and expansin activities may control the access of additional proteins to their substrates. A possible role for the apoplastic redox status in the modulation of the process is also discussedB. Belge was the recipient of a FI-DGR grant from AGAUR (Generalitat de Catalunya). This work was supported through the AGL2010-14801/ALI project, funded by the Ministerio de Ciencia e Innovación (MICINN) of Spain.Elsevier2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.scienta.2017.02.039http://hdl.handle.net/10459.1/59387reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2010-14801Versió postprint del document publicat a: https://doi.org/10.1016/j.scienta.2017.02.039Scientia Horticulturae, 2017, vol. 219, p. 182-190cc-by-nc-nd, (c) Elsevier, 2017info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/oai:repositori.udl.cat:10459.1/593872026-06-24T12:42:17Z
dc.title.none.fl_str_mv Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
title Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
spellingShingle Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
Belge, Burcu
Firmness loss
Sweet cherry
Gene expression
Prunus avium L.
Enzims
Fruita de pinyol
Enzymes
Stone fruit
title_short Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
title_full Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
title_fullStr Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
title_full_unstemmed Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
title_sort Refrigerated storage and calcium dips of ripe 'Celeste' sweet cherryfruit: combined effects on cell wall metabolism
dc.creator.none.fl_str_mv Belge, Burcu
Goulao, Luis F.
Comabella, Eva
Graell i Sarle, Jordi
Lara Ayala, Isabel
author Belge, Burcu
author_facet Belge, Burcu
Goulao, Luis F.
Comabella, Eva
Graell i Sarle, Jordi
Lara Ayala, Isabel
author_role author
author2 Goulao, Luis F.
Comabella, Eva
Graell i Sarle, Jordi
Lara Ayala, Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Firmness loss
Sweet cherry
Gene expression
Prunus avium L.
Enzims
Fruita de pinyol
Enzymes
Stone fruit
topic Firmness loss
Sweet cherry
Gene expression
Prunus avium L.
Enzims
Fruita de pinyol
Enzymes
Stone fruit
description Ripening-related firmness loss shortens considerably the storage potential of sweet cherry (Prunus avium L.), thus limiting postharvest handling, transportation, and commercialisation. The biochemical mechanisms underlying this process in cherries are not fully understood, and the mechanisms operating in a given fruit may be not extrapolated to a different species. Cell wall materials obtained from untreated and calcium-treated 'Celeste' sweet cherries were fractionated and analysed after cold storage, and related enzyme activities and gene expression were assessed. Calcium-treated fruit were firmer, with lower weight loss and decay incidence than the controls. The accumulation of PaβGal and PaEXP1 transcripts was strongly inhibited in cold-stored fruit, although expression levels recovered largely after three days of shelf life. Data suggest that pectin methylesterase, β-galactosidase and expansin activities may control the access of additional proteins to their substrates. A possible role for the apoplastic redox status in the modulation of the process is also discussed
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.scienta.2017.02.039
http://hdl.handle.net/10459.1/59387
url https://doi.org/10.1016/j.scienta.2017.02.039
http://hdl.handle.net/10459.1/59387
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2010-14801
Versió postprint del document publicat a: https://doi.org/10.1016/j.scienta.2017.02.039
Scientia Horticulturae, 2017, vol. 219, p. 182-190
dc.rights.none.fl_str_mv cc-by-nc-nd, (c) Elsevier, 2017
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
rights_invalid_str_mv cc-by-nc-nd, (c) Elsevier, 2017
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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