Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw

Lignin biodegradation is an oxidative process involving the action of fungal enzymes and the subsequent chemical reactions of aromatic radicals formed. Aromatic acid release has been reported during biodegradation of lignocellulosic materials, suggesting oxidative degradation of side chains in ligni...

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Autores: Camarero, Susana, Galletti, Guido C., Martínez, Ángel T.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1998
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::c60ff92bcb024f975b1dbb83f6dbf0c7
Acceso en línea:http://hdl.handle.net/10261/429402
Access Level:acceso abierto
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spelling Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat StrawCamarero, SusanaGalletti, Guido C.Martínez, Ángel T.Lignin biodegradation is an oxidative process involving the action of fungal enzymes and the subsequent chemical reactions of aromatic radicals formed. Aromatic acid release has been reported during biodegradation of lignocellulosic materials, suggesting oxidative degradation of side chains in lignin units. In the present study, wheat lignin degradation by two basidiomycetes was investigated by pyrolysis coupled to GC/MS after sample permethylation. In this way, analysis of phenolic and etherified lignin units, yielding C4-OMe and C4-OH compounds respectively, was performed. The amount of etherified residues of carboxy-free vanillic and syringic acids was found to be very low in sound straw. However, the molar abundances of these acid residues increased strongly during fungal degradation of lignin. For example, each of them attained 8% of total lignin-derived compounds recovered after pyrolysis of straw treated with Pleurotus eryngii (after 50% lignin degradation). These findings support Ca-Cß cleavage as one of the most frequent reactions during lignin biodegradation, and constitute evidence for in situ cleavage of lignin side chains during fungal attack to this macromolecule.This work was partially funded by the European Union project ‘‘Biological delignification in paper manufacture’’ (AIR2-CT93-1219) and the Spanish Biotechnology Programme. Py-GC/MS was carried out at the CCF/CNR, Bologna.Peer reviewedJohn Wiley & SonsEuropean CommissionCamarero, Susana [0000-0002-2812-895X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620261998info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/429402reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1002/(SICI)1097-0231(19970228)11:4%3C331::AID-RCM844%3E3.0.CO;2-HSíinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::c60ff92bcb024f975b1dbb83f6dbf0c72026-05-22T06:33:51Z
dc.title.none.fl_str_mv Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
title Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
spellingShingle Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
Camarero, Susana
title_short Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
title_full Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
title_fullStr Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
title_full_unstemmed Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
title_sort Demonstration of In Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw
dc.creator.none.fl_str_mv Camarero, Susana
Galletti, Guido C.
Martínez, Ángel T.
author Camarero, Susana
author_facet Camarero, Susana
Galletti, Guido C.
Martínez, Ángel T.
author_role author
author2 Galletti, Guido C.
Martínez, Ángel T.
author2_role author
author
dc.contributor.none.fl_str_mv European Commission
Camarero, Susana [0000-0002-2812-895X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Lignin biodegradation is an oxidative process involving the action of fungal enzymes and the subsequent chemical reactions of aromatic radicals formed. Aromatic acid release has been reported during biodegradation of lignocellulosic materials, suggesting oxidative degradation of side chains in lignin units. In the present study, wheat lignin degradation by two basidiomycetes was investigated by pyrolysis coupled to GC/MS after sample permethylation. In this way, analysis of phenolic and etherified lignin units, yielding C4-OMe and C4-OH compounds respectively, was performed. The amount of etherified residues of carboxy-free vanillic and syringic acids was found to be very low in sound straw. However, the molar abundances of these acid residues increased strongly during fungal degradation of lignin. For example, each of them attained 8% of total lignin-derived compounds recovered after pyrolysis of straw treated with Pleurotus eryngii (after 50% lignin degradation). These findings support Ca-Cß cleavage as one of the most frequent reactions during lignin biodegradation, and constitute evidence for in situ cleavage of lignin side chains during fungal attack to this macromolecule.
publishDate 1998
dc.date.none.fl_str_mv 1998
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/429402
url http://hdl.handle.net/10261/429402
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1002/(SICI)1097-0231(19970228)11:4%3C331::AID-RCM844%3E3.0.CO;2-H
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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