Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis

Ramos, Teresa L.; Sánchez Abarca, Luis Ignacio; Rosón Burgo, Beatriz; Redondo Guijo, Alba María; Rico, Ana; Preciado Pérez, Silvia; Ortega, Rebeca; Rodríguez, Concepción; Muntión Olave, María Sandra; Hernández Hernández, Ángel; Rivas Sanz, Javier de las; González, Marcos; González Porras, José Ramón; Cañizo Fernández-Roldán, María Consuelo del; Sánchez-Guijo Martín, Fermín

Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis

[EN]There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expr...

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Autores:	Ramos, Teresa L., Sánchez Abarca, Luis Ignacio, Rosón Burgo, Beatriz, Redondo Guijo, Alba María, Rico, Ana, Preciado Pérez, Silvia, Ortega, Rebeca, Rodríguez, Concepción, Muntión Olave, María Sandra, Hernández Hernández, Ángel, Rivas Sanz, Javier de las, González, Marcos, González Porras, José Ramón, Cañizo Fernández-Roldán, María Consuelo del, Sánchez-Guijo Martín, Fermín
Tipo de recurso:	artículo
Fecha de publicación:	2017
País:	España
Institución:	Universidad de Salamanca (USAL)
Repositorio:	GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:	oai:gredos.usal.es:10366/135282
Acceso en línea:	http://hdl.handle.net/10366/135282 https://doi.org/10.1371/journal.pone.0182470
Access Level:	acceso abierto
Palabra clave:	Mesenchymal stromal cells Cell biology Neoplastic hematopoiesis Células madre Neoplasias hematopoyeticas

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network_name_str	España
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spelling	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesisRamos, Teresa L.Sánchez Abarca, Luis IgnacioRosón Burgo, BeatrizRedondo Guijo, Alba MaríaRico, AnaPreciado Pérez, SilviaOrtega, RebecaRodríguez, ConcepciónMuntión Olave, María SandraHernández Hernández, ÁngelRivas Sanz, Javier de lasGonzález, MarcosGonzález Porras, José RamónCañizo Fernández-Roldán, María Consuelo delSánchez-Guijo Martín, FermínMesenchymal stromal cellsCell biologyNeoplastic hematopoiesisCélulas madreNeoplasias hematopoyeticas[EN]There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HDMSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cellsPublic Library of Science (New York)201720172017info:eu-repo/semantics/articlehttp://hdl.handle.net/10366/135282https://doi.org/10.1371/journal.pone.0182470reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivs 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1352822026-06-07T06:28:51Z
dc.title.none.fl_str_mv	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
title	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
spellingShingle	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis Ramos, Teresa L. Mesenchymal stromal cells Cell biology Neoplastic hematopoiesis Células madre Neoplasias hematopoyeticas
title_short	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
title_full	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
title_fullStr	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
title_full_unstemmed	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
title_sort	Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
dc.creator.none.fl_str_mv	Ramos, Teresa L. Sánchez Abarca, Luis Ignacio Rosón Burgo, Beatriz Redondo Guijo, Alba María Rico, Ana Preciado Pérez, Silvia Ortega, Rebeca Rodríguez, Concepción Muntión Olave, María Sandra Hernández Hernández, Ángel Rivas Sanz, Javier de las González, Marcos González Porras, José Ramón Cañizo Fernández-Roldán, María Consuelo del Sánchez-Guijo Martín, Fermín
author	Ramos, Teresa L.
author_facet	Ramos, Teresa L. Sánchez Abarca, Luis Ignacio Rosón Burgo, Beatriz Redondo Guijo, Alba María Rico, Ana Preciado Pérez, Silvia Ortega, Rebeca Rodríguez, Concepción Muntión Olave, María Sandra Hernández Hernández, Ángel Rivas Sanz, Javier de las González, Marcos González Porras, José Ramón Cañizo Fernández-Roldán, María Consuelo del Sánchez-Guijo Martín, Fermín
author_role	author
author2	Sánchez Abarca, Luis Ignacio Rosón Burgo, Beatriz Redondo Guijo, Alba María Rico, Ana Preciado Pérez, Silvia Ortega, Rebeca Rodríguez, Concepción Muntión Olave, María Sandra Hernández Hernández, Ángel Rivas Sanz, Javier de las González, Marcos González Porras, José Ramón Cañizo Fernández-Roldán, María Consuelo del Sánchez-Guijo Martín, Fermín
author2_role	author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Mesenchymal stromal cells Cell biology Neoplastic hematopoiesis Células madre Neoplasias hematopoyeticas
topic	Mesenchymal stromal cells Cell biology Neoplastic hematopoiesis Células madre Neoplasias hematopoyeticas
description	[EN]There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HDMSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cells
publishDate	2017
dc.date.none.fl_str_mv	2017 2017 2017
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10366/135282 https://doi.org/10.1371/journal.pone.0182470
url	http://hdl.handle.net/10366/135282 https://doi.org/10.1371/journal.pone.0182470
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.rights.none.fl_str_mv	Attribution-NonCommercial-NoDerivs 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Attribution-NonCommercial-NoDerivs 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science (New York)
publisher.none.fl_str_mv	Public Library of Science (New York)
dc.source.none.fl_str_mv	reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL)
instname_str	Universidad de Salamanca (USAL)
reponame_str	GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection	GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.301603

Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis

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