Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry

For the first time, a capillary electrophoresis-time of flight-mass spectrometry analysis method for detecting anthocyanins in red onion was developed. The analysis method included the use of silica capillaries coated with poly-LA 313 (polycationic amine-containing polymer) and an MS-compatible vola...

ver descrição completa

Detalhes bibliográficos
Autores: Petersson, Erik V., Puerta, Angel de la, Bergquist, Jonas, Turner, Charlotta
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2008
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346628
Acesso em linha:http://hdl.handle.net/10261/346628
Access Level:Acceso aberto
Palavra-chave:Anthocyanins
Capillary electrophoresis
Polyamine coating
Time of flight mass spectrometry
Red onion
id ES_e5b9a67b7fbdf4a60206665de3440f52
oai_identifier_str oai:digital.csic.es:10261/346628
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometryPetersson, Erik V.Puerta, Angel de laBergquist, JonasTurner, CharlottaAnthocyaninsCapillary electrophoresisPolyamine coatingTime of flight mass spectrometryRed onionFor the first time, a capillary electrophoresis-time of flight-mass spectrometry analysis method for detecting anthocyanins in red onion was developed. The analysis method included the use of silica capillaries coated with poly-LA 313 (polycationic amine-containing polymer) and an MS-compatible volatile background electrolyte (BGE). The method was environmentally friendly and sensitive; and its rapidness combined with an acidic BGE helped in preventing anthocyanin degradation. By using high-resolution TOF-MS with prerun tuning of masses, low mass errors were achieved in the determination of conjugated anthocyanins in red onion, and a simultaneous up-front fragmentation provided confirmation of the aglycon backbone for their secure identification. Most anthocyanins (at least seven out of ten) known in red onion from the literature were found, as well as one new for this matrix.Angel Puerta acknowledges Spanish Ministry of Educationand Science for a postdoctoral grant to perform research in Swe-den. The Swedish Research Council is acknowledged for financial support (621-2005-5279, 629-2002-6821 J.B.). Professor Jonas Bergquist holds a senior research position financed by the Swedish Research Council. Astra Zeneca is acknowledged for the loan ofthe CE system. Charlotta Turner acknowledges the Swedish Re-search Council for Environment, Agricultural Sciences and Spa-tial Planning (FORMAS, 209-2006-1346); the Swedish Re-search Council (VR, 2006-4084); and the Swedish Foundationfor Strategic Research (SSF, 2005:0073/13).Peer reviewedWiley-VCHMinisterio de Educación y Ciencia (España)Swedish Research CouncilSwedish Foundation for Strategic ResearchConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420082024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/346628reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/elps.200700692Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3466282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
title Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
spellingShingle Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
Petersson, Erik V.
Anthocyanins
Capillary electrophoresis
Polyamine coating
Time of flight mass spectrometry
Red onion
title_short Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
title_full Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
title_fullStr Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
title_full_unstemmed Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
title_sort Analysis of anthocyanins in red onion usingcapillary electrophoresis-time of flight-mass spectrometry
dc.creator.none.fl_str_mv Petersson, Erik V.
Puerta, Angel de la
Bergquist, Jonas
Turner, Charlotta
author Petersson, Erik V.
author_facet Petersson, Erik V.
Puerta, Angel de la
Bergquist, Jonas
Turner, Charlotta
author_role author
author2 Puerta, Angel de la
Bergquist, Jonas
Turner, Charlotta
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Swedish Research Council
Swedish Foundation for Strategic Research
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anthocyanins
Capillary electrophoresis
Polyamine coating
Time of flight mass spectrometry
Red onion
topic Anthocyanins
Capillary electrophoresis
Polyamine coating
Time of flight mass spectrometry
Red onion
description For the first time, a capillary electrophoresis-time of flight-mass spectrometry analysis method for detecting anthocyanins in red onion was developed. The analysis method included the use of silica capillaries coated with poly-LA 313 (polycationic amine-containing polymer) and an MS-compatible volatile background electrolyte (BGE). The method was environmentally friendly and sensitive; and its rapidness combined with an acidic BGE helped in preventing anthocyanin degradation. By using high-resolution TOF-MS with prerun tuning of masses, low mass errors were achieved in the determination of conjugated anthocyanins in red onion, and a simultaneous up-front fragmentation provided confirmation of the aglycon backbone for their secure identification. Most anthocyanins (at least seven out of ten) known in red onion from the literature were found, as well as one new for this matrix.
publishDate 2008
dc.date.none.fl_str_mv 2008
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346628
url http://hdl.handle.net/10261/346628
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/elps.200700692
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422704752328704
score 15.812429

Registros relacionados