Reversible photocontrol of dopaminergic transmission in wild-type animals

Understanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available t...

Descripción completa

Detalles Bibliográficos
Autores: Matera, Carlo, Calvé, Pablo, Casadó Anguera, Verònica, Sortino, Rosalba, Gomila, Alexandre M. J., Moreno Guillén, Estefanía, Gener, Thomas, Delgado-Sallent, Cristina, Nebot, Pau, Costazza, Davide, Conde-Berriozábal, Sara, Masana Nadal, Mercè, Hernando, Jordi, Casadó, Vicent, Puig, M. Victoria, Gorostiza Langa, Pablo Ignacio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/189693
Acceso en línea:https://hdl.handle.net/2445/189693
Access Level:acceso abierto
Palabra clave:Dopamina
Electrofisiologia
Dopamine
Electrophysiology
id ES_e5ae31c0997492b9e8d2da1eea86c4c8
oai_identifier_str oai:diposit.ub.edu:2445/189693
network_acronym_str ES
network_name_str España
repository_id_str
spelling Reversible photocontrol of dopaminergic transmission in wild-type animalsMatera, CarloCalvé, PabloCasadó Anguera, VerònicaSortino, RosalbaGomila, Alexandre M. J. Moreno Guillén, EstefaníaGener, ThomasDelgado-Sallent, CristinaNebot, PauCostazza, DavideConde-Berriozábal, SaraMasana Nadal, MercèHernando, JordiCasadó, VicentPuig, M. VictoriaGorostiza Langa, Pablo IgnacioDopaminaElectrofisiologiaDopamineElectrophysiologyUnderstanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available tools to dissect the endogenous dopaminergic circuits have limited specificity, reversibility, resolution, or require genetic manipulation. Here we introduce azodopa, a novel photoswitchable ligand that enables reversible spatiotemporal control of dopaminergic transmission. We demonstrate that azodopa activates D1-like receptors in vitro in a light-dependent manner. Moreover, it enables reversibly photocontrolling zebrafish motility on a time scale of seconds and allows separating the retinal component of dopaminergic neurotransmission. Azodopa increases the overall neural activity in the cortex of anesthetized mice and displays illuminationdependent activity in individual cells. Azodopa is the first photoswitchable dopamine agonist with demonstrated efficacy in wildtype animals and opens the way to remotely controlling dopaminergic neurotransmission for fundamental and therapeutic purposes.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/189693Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms231710114International Journal of Molecular Sciences, 2022, vol. 23, num. 10114, p. 1-18https://doi.org/10.3390/ijms231710114info:eu-repo/grantAgreement/EC/H2020/101016787cc-by (c) Matera, Carlo et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1896932026-05-27T06:46:51Z
dc.title.none.fl_str_mv Reversible photocontrol of dopaminergic transmission in wild-type animals
title Reversible photocontrol of dopaminergic transmission in wild-type animals
spellingShingle Reversible photocontrol of dopaminergic transmission in wild-type animals
Matera, Carlo
Dopamina
Electrofisiologia
Dopamine
Electrophysiology
title_short Reversible photocontrol of dopaminergic transmission in wild-type animals
title_full Reversible photocontrol of dopaminergic transmission in wild-type animals
title_fullStr Reversible photocontrol of dopaminergic transmission in wild-type animals
title_full_unstemmed Reversible photocontrol of dopaminergic transmission in wild-type animals
title_sort Reversible photocontrol of dopaminergic transmission in wild-type animals
dc.creator.none.fl_str_mv Matera, Carlo
Calvé, Pablo
Casadó Anguera, Verònica
Sortino, Rosalba
Gomila, Alexandre M. J.
Moreno Guillén, Estefanía
Gener, Thomas
Delgado-Sallent, Cristina
Nebot, Pau
Costazza, Davide
Conde-Berriozábal, Sara
Masana Nadal, Mercè
Hernando, Jordi
Casadó, Vicent
Puig, M. Victoria
Gorostiza Langa, Pablo Ignacio
author Matera, Carlo
author_facet Matera, Carlo
Calvé, Pablo
Casadó Anguera, Verònica
Sortino, Rosalba
Gomila, Alexandre M. J.
Moreno Guillén, Estefanía
Gener, Thomas
Delgado-Sallent, Cristina
Nebot, Pau
Costazza, Davide
Conde-Berriozábal, Sara
Masana Nadal, Mercè
Hernando, Jordi
Casadó, Vicent
Puig, M. Victoria
Gorostiza Langa, Pablo Ignacio
author_role author
author2 Calvé, Pablo
Casadó Anguera, Verònica
Sortino, Rosalba
Gomila, Alexandre M. J.
Moreno Guillén, Estefanía
Gener, Thomas
Delgado-Sallent, Cristina
Nebot, Pau
Costazza, Davide
Conde-Berriozábal, Sara
Masana Nadal, Mercè
Hernando, Jordi
Casadó, Vicent
Puig, M. Victoria
Gorostiza Langa, Pablo Ignacio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dopamina
Electrofisiologia
Dopamine
Electrophysiology
topic Dopamina
Electrofisiologia
Dopamine
Electrophysiology
description Understanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available tools to dissect the endogenous dopaminergic circuits have limited specificity, reversibility, resolution, or require genetic manipulation. Here we introduce azodopa, a novel photoswitchable ligand that enables reversible spatiotemporal control of dopaminergic transmission. We demonstrate that azodopa activates D1-like receptors in vitro in a light-dependent manner. Moreover, it enables reversibly photocontrolling zebrafish motility on a time scale of seconds and allows separating the retinal component of dopaminergic neurotransmission. Azodopa increases the overall neural activity in the cortex of anesthetized mice and displays illuminationdependent activity in individual cells. Azodopa is the first photoswitchable dopamine agonist with demonstrated efficacy in wildtype animals and opens the way to remotely controlling dopaminergic neurotransmission for fundamental and therapeutic purposes.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/189693
url https://hdl.handle.net/2445/189693
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms231710114
International Journal of Molecular Sciences, 2022, vol. 23, num. 10114, p. 1-18
https://doi.org/10.3390/ijms231710114
info:eu-repo/grantAgreement/EC/H2020/101016787
dc.rights.none.fl_str_mv cc-by (c) Matera, Carlo et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Matera, Carlo et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422700789760000
score 15,301603

Similares en LA Referencia