Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation

15 páginas.- 6 figuras.- 5 tablas.- 49 referencias.- (This article belongs to the Special Issue Feature Collection in Environmental Microbiology Section 2021-2022)

Detalles Bibliográficos
Autores: Vargas-Ordóñez, Antonio, Aguilar Romero, Inés María, Villaverde Capellán, J., Madrid Díaz, Fernando, Morillo González, Esmeralda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/287750
Acceso en línea:http://hdl.handle.net/10261/287750
Access Level:acceso abierto
Palabra clave:Biodegradation
Paracetamol degrader
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
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oai_identifier_str oai:digital.csic.es:10261/287750
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
title Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
spellingShingle Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
Vargas-Ordóñez, Antonio
Biodegradation
Paracetamol degrader
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
title_short Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
title_full Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
title_fullStr Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
title_full_unstemmed Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
title_sort Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation
dc.creator.none.fl_str_mv Vargas-Ordóñez, Antonio
Aguilar Romero, Inés María
Villaverde Capellán, J.
Madrid Díaz, Fernando
Morillo González, Esmeralda
author Vargas-Ordóñez, Antonio
author_facet Vargas-Ordóñez, Antonio
Aguilar Romero, Inés María
Villaverde Capellán, J.
Madrid Díaz, Fernando
Morillo González, Esmeralda
author_role author
author2 Aguilar Romero, Inés María
Villaverde Capellán, J.
Madrid Díaz, Fernando
Morillo González, Esmeralda
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Junta de Andalucía
Vargas-Ordoñez, Antonio [0000-0001-6909-8075]
Aguilar Romero, Inés María [0000-0001-7786-8172]
Villaverde Capellán, J. [0000-0002-8694-7929]
Madrid Díaz, Fernando [0000-0002-2921-3515]
Morillo González, Esmeralda [0000-0002-4485-2315]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biodegradation
Paracetamol degrader
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
topic Biodegradation
Paracetamol degrader
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
description 15 páginas.- 6 figuras.- 5 tablas.- 49 referencias.- (This article belongs to the Special Issue Feature Collection in Environmental Microbiology Section 2021-2022)
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/287750
url http://hdl.handle.net/10261/287750
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2017-82472-C2-1-R
http://dx.doi.org/10.3390/microorganisms11010196
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422693155078144
spelling Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol BiodegradationVargas-Ordóñez, AntonioAguilar Romero, Inés MaríaVillaverde Capellán, J.Madrid Díaz, FernandoMorillo González, EsmeraldaBiodegradationParacetamol degraderSewage sludgePseudomonas extremaustralisStutzerimonas stutzeri15 páginas.- 6 figuras.- 5 tablas.- 49 referencias.- (This article belongs to the Special Issue Feature Collection in Environmental Microbiology Section 2021-2022)first_pagesettingsOrder Article Reprints Open AccessArticle Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation by Antonio Vargas-OrdóñezORCID,Inés Aguilar-Romero,Jaime VillaverdeORCID,Fernando MadridORCID andEsmeralda Morillo *ORCID Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC), 41012 Seville, Spain * Author to whom correspondence should be addressed. Microorganisms 2023, 11(1), 196; https://doi.org/10.3390/microorganisms11010196 Received: 23 December 2022 / Revised: 10 January 2023 / Accepted: 11 January 2023 / Published: 12 January 2023 (This article belongs to the Special Issue Feature Collection in Environmental Microbiology Section 2021-2022) Download Browse Figures Versions Notes Abstract Paracetamol is one of the most used pharmaceuticals worldwide, but due to its widespread use it is detected in various environmental matrices, such as surface and ground waters, sediments, soils or even plants, where it is introduced mainly from the discharge of wastewater and the use of sewage sludge as fertilizer in agriculture. Its accumulation in certain organisms can induce reproductive, neurotoxic or endocrine disorders, being therefore considered an emerging pollutant. This study reports on the isolation, from sewage sludge produced in wastewater treatment plants (WWTPs), of bacterial strains capable of degrading paracetamol. Up to 17 bacterial strains were isolated, but only two of them, identified as Pseudomonas stutzeri CSW02 and Pseudomonas extremaustralis CSW01, were able to degrade very high concentrations of paracetamol in solution as a sole carbon and energy source, and none of them had been previously described as paracetamol degraders. These bacteria showed the ability to degrade up to 500 mg L−1 of paracetamol in only 6 and 4 h, respectively, much quicker than any other paracetamol-degrader strain described in the literature. The two main paracetamol metabolites, 4-aminophenol and hydroquinone, which present high toxicity, were detected during the degradation process, although they disappeared very quickly for paracetamol concentrations up to 500 mg L−1. The IC50 of paracetamol for the growth of these two isolates was also calculated, indicating that P. extremaustralis CSW01 was more tolerant than S. stutzeri CSW02 to high concentrations of paracetamol and/or its metabolites in solution, and this is the reason for the much lower paracetamol degradation by S. stutzeri CSW02 at 2000–3000 mg L−1. These findings indicate that both bacteria are very promising candidates for their use in paracetamol bioremediation in water and sewage sludge.This work was supported by the Spanish Ministry of Science, Innovation and Universities under the research project CTM2017-82472-C2-1-R (AEI/FEDER, UE), and by the Junta de Andalucía (Council of Economic Transformation, Industry, Knowledge and University) under the research project PY20_01069 (FEDER Andalucía PO 2014-2020), and by the A.V.-O. Youth Employment fellowship (AND21_IRNAS_M2_158).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionJunta de AndalucíaVargas-Ordoñez, Antonio [0000-0001-6909-8075]Aguilar Romero, Inés María [0000-0001-7786-8172]Villaverde Capellán, J. [0000-0002-8694-7929]Madrid Díaz, Fernando [0000-0002-2921-3515]Morillo González, Esmeralda [0000-0002-4485-2315]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/287750reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2017-82472-C2-1-Rhttp://dx.doi.org/10.3390/microorganisms11010196Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2877502026-05-22T06:33:51Z
score 15,812429

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