Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum

[EN] Industrial penicillin production with the filamentous fungus Penicillium chrysogenum is based on an unprecedented effort in microbial strain improvement. To gain more insight into penicillin synthesis, we sequenced the 32.19 Mb genome of P. chrysogenum Wisconsin54-1255 and identified numerous g...

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Detalles Bibliográficos
Autores: Bovenberg, Roel A L, Daran, Jean Marc, M Driessen, Arnold J, García Estrada, Carlos, Fedorova, Natalie D, Harris, Diana M, Heijne, Wilbert H M, Joardar, Vinita, W Kiel, Jan A K, Kovalchuk, Andriy, Martín Martín, Juan Francisco, Nierman, William C, Nijland, Jeroen G, Pronk, Jack T, Roubos, Johannes A, van der Klei, Ida J, van Peij, Noël N M E, Veenhuis, Marten, von Döhren, Hans, Wagner, Christian, Wortman, Jennifer
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24273
Acceso en línea:https://www.nature.com/articles/nbt.1498
https://hdl.handle.net/10612/24273
Access Level:acceso abierto
Palabra clave:Toxicología
Genome sequencing
Penicillium chrysogenum
Descripción
Sumario:[EN] Industrial penicillin production with the filamentous fungus Penicillium chrysogenum is based on an unprecedented effort in microbial strain improvement. To gain more insight into penicillin synthesis, we sequenced the 32.19 Mb genome of P. chrysogenum Wisconsin54-1255 and identified numerous genes responsible for key steps in penicillin production. DNA microarrays were used to compare the transcriptomes of the sequenced strain and a penicillinG high-producing strain, grown in the presence and absence of the side-chain precursor phenylacetic acid. Transcription of genes involved in biosynthesis of valine, cysteine and α-aminoadipic acid—precursors for penicillin biosynthesis—as well as of genes encoding microbody proteins, was increased in the high-producing strain. Some gene products were shown to be directly controlling β-lactam output. Many key cellular transport processes involving penicillins and intermediates remain to be characterized at the molecular level. Genes predicted to encode transporters were strongly overrepresented among the genes transcriptionally upregulated under conditions that stimulate penicillinG production, illustrating potential for future genomics-driven metabolic engineering.

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