Ribosome-associated Vesicles: A Dynamic Subcompartment of the Endoplasmic Reticulum in Secretory Cells

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these im...

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Detalles Bibliográficos
Autores: Carter, Stephen D., Hampton, Cheri M., Langlois, Robert, Melero, Roberto, Zachary J. Farino, Calderon, Michael J., Li, Wen, Wallace, Callen T., ran, Ngoc Han, Grassucci, Robert A., Siegmund, Stephanie E., Pemberton, Joshua, Morgenstern, Travis J., Eisenman, Leanna, Aguilar, Jenny I., Greenberg, Nili L., Levy, Elana S., Yi, Edward, Mitchell, William G., Rice, William J., Pilli, Jyotsna, George, Emily W., Aslanoglou, Despoina, Courel, Maïté, Freyberg, Robin J., Javitch, Jonathan A., Wills, Zachary P., Area-Gomez, Estela, Shiva, Sruti, Bartolini, Francesca, Volchuk, Allen, Murray, Sandra A., Aridor, Meir, Fish, Kenneth N., Walter, Peter, Balla, Tamas, Fass, Deborah, Wolf, Sharon G., Watkins, Simon C., Carazo, José M., Jensen, Grant J., Frank, Joachim, Freyberg, Zachary
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/212919
Acceso en línea:http://hdl.handle.net/10261/212919
Access Level:acceso abierto
Descripción
Sumario:The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.