Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development

Free and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum),...

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Autores: Chávez, Ángel, Castillo, Nidia, López-Tubau, Joan Manel, Atanasov, Kostadin E., Fernández-Crespo, Emma, Camañes, Gemma, Altabella, Teresa, Ferrer, Albert
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/335952
Acceso en línea:http://hdl.handle.net/10261/335952
https://api.elsevier.com/content/abstract/scopus_id/85144022912
Access Level:acceso abierto
Palabra clave:Artificial microRNA
Conjugated sterols
Gene silencing
Phytosterol metabolism
Solanum lycopersicum
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spelling Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit developmentChávez, ÁngelCastillo, NidiaLópez-Tubau, Joan ManelAtanasov, Kostadin E.Fernández-Crespo, EmmaCamañes, GemmaAltabella, TeresaFerrer, AlbertArtificial microRNAConjugated sterolsGene silencingPhytosterol metabolismSolanum lycopersicumFree and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum), in which glycosylated sterols are the predominant sterols. A proper ratio of free sterols versus glycosylated sterols has proven to be essential for proper plant performance in several species, but almost nothing is known in tomato. To assess the role of glycosylated sterols in tomato plant and fruit development, we generated transgenic lines of tomato cultivar Micro-Tom expressing two different amiRNAs devised to silence STEROL GLYCOSYLTRANSFERASE 1, the most actively expressed of the four genes encoding sterol glycosyltransferases in this plant. STEROL GLYCOSYLTRANSFERASE 1 gene silencing caused moderate plant dwarfism and reduced fruit size. Analysis of the profile of glycosylated sterols throughout fruit development demonstrated that the maintenance of proper levels of these compounds during the early stages of fruit development is essential for normal fruit growth, since reduced levels of glycosylated sterols trigger a transcriptional downregulatory response that affects genes involved in processes that are critical for proper fruit development, such as seed filling, cell wall extension and auxin signaling.This work was supported by grants AGL2017-88842-R and PID2021-126591OB-I00 funded by MCIN/AEI/10.13039/501100011033, FEDER, a way of making Europe (Spain), and grant 2017SGR710 from the Generalitat de Catalunya. This work was also supported by grants CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033 (Spain) and by the CERCA Programme of the Generalitat de Catalunya.With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000902-S)Peer reviewedElsevier BVMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionGeneralitat de CatalunyaMinisterio de Ciencia, Innovación y Universidades (España)Ferrer, Albert [0000-0002-0741-2388]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/335952https://api.elsevier.com/content/abstract/scopus_id/85144022912reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88842-Rinfo:eu-repo/grantAgreement/AEI//PID2021-126591OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000902-SThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.envexpbot.2022.105181https://doi.org/10.1016/j.envexpbot.2022.105181Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3359522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
spellingShingle Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
Chávez, Ángel
Artificial microRNA
Conjugated sterols
Gene silencing
Phytosterol metabolism
Solanum lycopersicum
title_short Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_full Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_fullStr Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_full_unstemmed Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_sort Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
dc.creator.none.fl_str_mv Chávez, Ángel
Castillo, Nidia
López-Tubau, Joan Manel
Atanasov, Kostadin E.
Fernández-Crespo, Emma
Camañes, Gemma
Altabella, Teresa
Ferrer, Albert
author Chávez, Ángel
author_facet Chávez, Ángel
Castillo, Nidia
López-Tubau, Joan Manel
Atanasov, Kostadin E.
Fernández-Crespo, Emma
Camañes, Gemma
Altabella, Teresa
Ferrer, Albert
author_role author
author2 Castillo, Nidia
López-Tubau, Joan Manel
Atanasov, Kostadin E.
Fernández-Crespo, Emma
Camañes, Gemma
Altabella, Teresa
Ferrer, Albert
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Generalitat de Catalunya
Ministerio de Ciencia, Innovación y Universidades (España)
Ferrer, Albert [0000-0002-0741-2388]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Artificial microRNA
Conjugated sterols
Gene silencing
Phytosterol metabolism
Solanum lycopersicum
topic Artificial microRNA
Conjugated sterols
Gene silencing
Phytosterol metabolism
Solanum lycopersicum
description Free and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum), in which glycosylated sterols are the predominant sterols. A proper ratio of free sterols versus glycosylated sterols has proven to be essential for proper plant performance in several species, but almost nothing is known in tomato. To assess the role of glycosylated sterols in tomato plant and fruit development, we generated transgenic lines of tomato cultivar Micro-Tom expressing two different amiRNAs devised to silence STEROL GLYCOSYLTRANSFERASE 1, the most actively expressed of the four genes encoding sterol glycosyltransferases in this plant. STEROL GLYCOSYLTRANSFERASE 1 gene silencing caused moderate plant dwarfism and reduced fruit size. Analysis of the profile of glycosylated sterols throughout fruit development demonstrated that the maintenance of proper levels of these compounds during the early stages of fruit development is essential for normal fruit growth, since reduced levels of glycosylated sterols trigger a transcriptional downregulatory response that affects genes involved in processes that are critical for proper fruit development, such as seed filling, cell wall extension and auxin signaling.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/335952
https://api.elsevier.com/content/abstract/scopus_id/85144022912
url http://hdl.handle.net/10261/335952
https://api.elsevier.com/content/abstract/scopus_id/85144022912
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88842-R
info:eu-repo/grantAgreement/AEI//PID2021-126591OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000902-S
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.envexpbot.2022.105181
https://doi.org/10.1016/j.envexpbot.2022.105181
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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