Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates

[EN] Glaesserella parasuis (Gp) is the etiological agent of Glässer's disease (GD), which causes important economic losses for the pig intensive production worldwide. This organism uses a smart protein-based receptor to acquire specifically iron from the porcine transferrin. This surface recept...

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Autores: González Fernández, Alba, Gutiérrez Martín, César Bernardo, Petrocchi Rilo, Maximo, Pérez Fernández, Esther, Miguélez Pérez, Ruben, Frandoloso, Rafael, Martínez Martínez, Sonia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/15704
Acesso em linha:http://hdl.handle.net/10612/15704
Access Level:acceso abierto
Palavra-chave:Sanidad animal
Glaesserella parasuis
Glässer's disease
Serotyping
Sequencing
Phylogenetic relationships
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spelling Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolatesGonzález Fernández, AlbaGutiérrez Martín, César BernardoPetrocchi Rilo, MaximoPérez Fernández, EstherMiguélez Pérez, RubenFrandoloso, RafaelMartínez Martínez, SoniaSanidad animalGlaesserella parasuisGlässer's diseaseSerotypingSequencingPhylogenetic relationships[EN] Glaesserella parasuis (Gp) is the etiological agent of Glässer's disease (GD), which causes important economic losses for the pig intensive production worldwide. This organism uses a smart protein-based receptor to acquire specifically iron from the porcine transferrin. This surface receptor consists of transferrin-binding protein A (TbpA) and transferrin-binding protein B (TbpB). TbpB has been considered the most promising antigen to formulate a based-protein vaccine with broad-spectrum of protection against GD. The purpose of our study was to determine the capsular diversity of Gp clinical isolates collected in different Spanish regions between 2018 and 2021. A total of 68 Gp isolates were recovered from porcine respiratory or systemic samples. A species-specific PCR based on tbpA gene, followed by multiplex PCR for typing Gp isolates were performed. Serovars 5, 10, 2, 4 and 1 were the most prevalent and involved almost 84% of isolates. TbpB amino acid sequences from 59 of these isolates were analyzed, and a total of ten clades could be established. All of them showed a wide diversity with respect to capsular type, anatomical isolation site and geographical origin, with minor exceptions. Regardless of the serovars, the in silico analysis of TbpB sequences revealed that a vaccine based on a TbpB recombinant protein could potentially prevent Glässer's disease outbreaks in Spain.SIElsevierSanidad AnimalFacultad de Veterinaria2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10612/15704reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/10.13039/501100011033http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/157042026-06-24T12:43:27Z
dc.title.none.fl_str_mv Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
title Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
spellingShingle Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
González Fernández, Alba
Sanidad animal
Glaesserella parasuis
Glässer's disease
Serotyping
Sequencing
Phylogenetic relationships
title_short Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
title_full Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
title_fullStr Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
title_full_unstemmed Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
title_sort Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates
dc.creator.none.fl_str_mv González Fernández, Alba
Gutiérrez Martín, César Bernardo
Petrocchi Rilo, Maximo
Pérez Fernández, Esther
Miguélez Pérez, Ruben
Frandoloso, Rafael
Martínez Martínez, Sonia
author González Fernández, Alba
author_facet González Fernández, Alba
Gutiérrez Martín, César Bernardo
Petrocchi Rilo, Maximo
Pérez Fernández, Esther
Miguélez Pérez, Ruben
Frandoloso, Rafael
Martínez Martínez, Sonia
author_role author
author2 Gutiérrez Martín, César Bernardo
Petrocchi Rilo, Maximo
Pérez Fernández, Esther
Miguélez Pérez, Ruben
Frandoloso, Rafael
Martínez Martínez, Sonia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Glaesserella parasuis
Glässer's disease
Serotyping
Sequencing
Phylogenetic relationships
topic Sanidad animal
Glaesserella parasuis
Glässer's disease
Serotyping
Sequencing
Phylogenetic relationships
description [EN] Glaesserella parasuis (Gp) is the etiological agent of Glässer's disease (GD), which causes important economic losses for the pig intensive production worldwide. This organism uses a smart protein-based receptor to acquire specifically iron from the porcine transferrin. This surface receptor consists of transferrin-binding protein A (TbpA) and transferrin-binding protein B (TbpB). TbpB has been considered the most promising antigen to formulate a based-protein vaccine with broad-spectrum of protection against GD. The purpose of our study was to determine the capsular diversity of Gp clinical isolates collected in different Spanish regions between 2018 and 2021. A total of 68 Gp isolates were recovered from porcine respiratory or systemic samples. A species-specific PCR based on tbpA gene, followed by multiplex PCR for typing Gp isolates were performed. Serovars 5, 10, 2, 4 and 1 were the most prevalent and involved almost 84% of isolates. TbpB amino acid sequences from 59 of these isolates were analyzed, and a total of ten clades could be established. All of them showed a wide diversity with respect to capsular type, anatomical isolation site and geographical origin, with minor exceptions. Regardless of the serovars, the in silico analysis of TbpB sequences revealed that a vaccine based on a TbpB recombinant protein could potentially prevent Glässer's disease outbreaks in Spain.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10612/15704
url http://hdl.handle.net/10612/15704
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/10.13039/501100011033
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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