ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer

Endometrial cancer (EC) is the sixth deadliest cancer in women. The depth of myometrial invasion is one of the most important prognostic factors, being directly associated with tumor recurrence and mortality. In this study, ALCAM, a previously described marker of EC recurrence, was studied by immuno...

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Autores: Devis, Laura, Martínez-García, Elena, Moiola, Cristian P., Quiles, Maria Teresa, Arbós, Maria Antonia, Stirbat, Tomita Vasilica, Brochard-Wyart, Françoise, García, Ángel, Alonso-Alconada, Lorena, Abal, Miguel, Díaz-Feijoo, Berta, Thomas, William, Dufour, Sylvie, Mancebo Moreno, Gemma, Alameda Quitllet, Francisco, Reventós, Jaume, Gil-Moreno, Antonio, Colas, Eva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/41886
Acceso en línea:http://hdl.handle.net/10230/41886
http://dx.doi.org/10.18632/oncotarget.24625
Access Level:acceso abierto
Palabra clave:ALCAM
ETV5
MMP-9
Endometrial cancer
Myometrial invasion
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spelling ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancerDevis, LauraMartínez-García, ElenaMoiola, Cristian P.Quiles, Maria TeresaArbós, Maria AntoniaStirbat, Tomita VasilicaBrochard-Wyart, FrançoiseGarcía, ÁngelAlonso-Alconada, LorenaAbal, MiguelDíaz-Feijoo, BertaThomas, WilliamDufour, SylvieMancebo Moreno, GemmaAlameda Quitllet, FranciscoReventós, JaumeGil-Moreno, AntonioColas, EvaALCAMETV5MMP-9Endometrial cancerMyometrial invasionEndometrial cancer (EC) is the sixth deadliest cancer in women. The depth of myometrial invasion is one of the most important prognostic factors, being directly associated with tumor recurrence and mortality. In this study, ALCAM, a previously described marker of EC recurrence, was studied by immunohistochemistry at the superficial and the invasive tumor areas from 116 EC patients with different degree of myometrial invasion and related to a set of relevant epithelial and mesenchymal markers. ALCAM expression presented a heterogeneous functionality depending on its localization, it correlated with epithelial markers (E-cadherin/β-catenin) at the superficial area, and with mesenchymal markers at the invasive front (COX-2, SNAIL, ETV5, and MMP-9). At the invasive front, ALCAM-negativity was an independent marker of myometrial invasion. This negativity, together with an increase of soluble ALCAM in uterine aspirates from patients with an invasive EC, and its positive correlation with MMP-9 levels, suggested that ALCAM shedding by MMP-9 occurs at the invasive front. In vivo and in vitro models of invasive EC were generated by ETV5-overexpression. In those, we demonstrated that ALCAM shedding was related to a more invasive pattern and that full-ALCAM recovery reverted most of the ETV5-cells mesenchymal abilities, partially through a p-ERK dependent-manner.Impact Journal201920192018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/41886http://dx.doi.org/10.18632/oncotarget.24625reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésCopyright : © 2018 Devis et al. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), https://creativecommons.org/licenses/by/3.0/, which permits unrestricted use and redistribution provided that the original author and source are credited.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/418862026-05-29T05:05:01Z
dc.title.none.fl_str_mv ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
title ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
spellingShingle ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
Devis, Laura
ALCAM
ETV5
MMP-9
Endometrial cancer
Myometrial invasion
title_short ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
title_full ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
title_fullStr ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
title_full_unstemmed ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
title_sort ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer
dc.creator.none.fl_str_mv Devis, Laura
Martínez-García, Elena
Moiola, Cristian P.
Quiles, Maria Teresa
Arbós, Maria Antonia
Stirbat, Tomita Vasilica
Brochard-Wyart, Françoise
García, Ángel
Alonso-Alconada, Lorena
Abal, Miguel
Díaz-Feijoo, Berta
Thomas, William
Dufour, Sylvie
Mancebo Moreno, Gemma
Alameda Quitllet, Francisco
Reventós, Jaume
Gil-Moreno, Antonio
Colas, Eva
author Devis, Laura
author_facet Devis, Laura
Martínez-García, Elena
Moiola, Cristian P.
Quiles, Maria Teresa
Arbós, Maria Antonia
Stirbat, Tomita Vasilica
Brochard-Wyart, Françoise
García, Ángel
Alonso-Alconada, Lorena
Abal, Miguel
Díaz-Feijoo, Berta
Thomas, William
Dufour, Sylvie
Mancebo Moreno, Gemma
Alameda Quitllet, Francisco
Reventós, Jaume
Gil-Moreno, Antonio
Colas, Eva
author_role author
author2 Martínez-García, Elena
Moiola, Cristian P.
Quiles, Maria Teresa
Arbós, Maria Antonia
Stirbat, Tomita Vasilica
Brochard-Wyart, Françoise
García, Ángel
Alonso-Alconada, Lorena
Abal, Miguel
Díaz-Feijoo, Berta
Thomas, William
Dufour, Sylvie
Mancebo Moreno, Gemma
Alameda Quitllet, Francisco
Reventós, Jaume
Gil-Moreno, Antonio
Colas, Eva
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALCAM
ETV5
MMP-9
Endometrial cancer
Myometrial invasion
topic ALCAM
ETV5
MMP-9
Endometrial cancer
Myometrial invasion
description Endometrial cancer (EC) is the sixth deadliest cancer in women. The depth of myometrial invasion is one of the most important prognostic factors, being directly associated with tumor recurrence and mortality. In this study, ALCAM, a previously described marker of EC recurrence, was studied by immunohistochemistry at the superficial and the invasive tumor areas from 116 EC patients with different degree of myometrial invasion and related to a set of relevant epithelial and mesenchymal markers. ALCAM expression presented a heterogeneous functionality depending on its localization, it correlated with epithelial markers (E-cadherin/β-catenin) at the superficial area, and with mesenchymal markers at the invasive front (COX-2, SNAIL, ETV5, and MMP-9). At the invasive front, ALCAM-negativity was an independent marker of myometrial invasion. This negativity, together with an increase of soluble ALCAM in uterine aspirates from patients with an invasive EC, and its positive correlation with MMP-9 levels, suggested that ALCAM shedding by MMP-9 occurs at the invasive front. In vivo and in vitro models of invasive EC were generated by ETV5-overexpression. In those, we demonstrated that ALCAM shedding was related to a more invasive pattern and that full-ALCAM recovery reverted most of the ETV5-cells mesenchymal abilities, partially through a p-ERK dependent-manner.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/41886
http://dx.doi.org/10.18632/oncotarget.24625
url http://hdl.handle.net/10230/41886
http://dx.doi.org/10.18632/oncotarget.24625
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Impact Journal
publisher.none.fl_str_mv Impact Journal
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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