Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots

<jats:p>An accurate and simple screening method has been developed for the determination of fluoroquinolone antibiotics. Carbon dots were synthesized by simple hydrothermal treatment as highly fluorescent nano-sensors. They were subsequently used in the synthesis of organic-based molecularly i...

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Autores: Ahmed Faried Abdel Hakiem, Urriza-Arsuaga, Idoia, Urraca Ruiz, Javier
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/104809
Acceso en línea:https://hdl.handle.net/20.500.14352/104809
Access Level:acceso abierto
Palabra clave:54
fluoroquinolones
molecularly imprinted polymers
carbon dots
nano-composites
fluorescence quenching
meat samples
Ciencias
23 Química
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repository_id_str
spelling Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon DotsAhmed Faried Abdel HakiemUrriza-Arsuaga, IdoiaUrraca Ruiz, Javier54fluoroquinolonesmolecularly imprinted polymerscarbon dotsnano-compositesfluorescence quenchingmeat samplesCiencias23 Química<jats:p>An accurate and simple screening method has been developed for the determination of fluoroquinolone antibiotics. Carbon dots were synthesized by simple hydrothermal treatment as highly fluorescent nano-sensors. They were subsequently used in the synthesis of organic-based molecularly imprinted polymers to develop fluorescence-based polymeric composites using enoxacin as a representative dummy template molecule of fluoroquinolones. The method was optimized concerning the pH of the medium and composite concentration. The normalized fluorescence intensity showed efficient quenching under optimized conditions upon successive addition of the template, with an excellent correlation coefficient. The proposed method was applied to eight other fluoroquinolones, exhibiting, in all cases, good correlation coefficients (0.65–0.992) within the same linearity range (0.03–2.60 mg mL−1). Excellent detection and quantification limits were been obtained for the target analytes down to 0.062 and 0.186 mg L−1, respectively. All studied analytes showed no interference with enrofloxacin, the most commonly used veterinary fluoroquinolone, with a percentage of cross-reactivity varying from 89.00 to 540.00%. This method was applied successfully for the determination of enrofloxacin in three different types of meat samples: beef, pork, and chicken, with good recoveries varying from 70 to 100% at three levels. This new procedure is an easy analytical method that can be useful as a screening method for monitoring the environmental hazard of fluoroquinolones in quality control laboratories.</jats:p>MDPIUniversidad Complutense de Madrid20232023-11-0720232023-11-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/104809reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1048092026-06-02T12:44:21Z
dc.title.none.fl_str_mv Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
title Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
spellingShingle Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
Ahmed Faried Abdel Hakiem
54
fluoroquinolones
molecularly imprinted polymers
carbon dots
nano-composites
fluorescence quenching
meat samples
Ciencias
23 Química
title_short Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
title_full Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
title_fullStr Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
title_full_unstemmed Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
title_sort Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
dc.creator.none.fl_str_mv Ahmed Faried Abdel Hakiem
Urriza-Arsuaga, Idoia
Urraca Ruiz, Javier
author Ahmed Faried Abdel Hakiem
author_facet Ahmed Faried Abdel Hakiem
Urriza-Arsuaga, Idoia
Urraca Ruiz, Javier
author_role author
author2 Urriza-Arsuaga, Idoia
Urraca Ruiz, Javier
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 54
fluoroquinolones
molecularly imprinted polymers
carbon dots
nano-composites
fluorescence quenching
meat samples
Ciencias
23 Química
topic 54
fluoroquinolones
molecularly imprinted polymers
carbon dots
nano-composites
fluorescence quenching
meat samples
Ciencias
23 Química
description <jats:p>An accurate and simple screening method has been developed for the determination of fluoroquinolone antibiotics. Carbon dots were synthesized by simple hydrothermal treatment as highly fluorescent nano-sensors. They were subsequently used in the synthesis of organic-based molecularly imprinted polymers to develop fluorescence-based polymeric composites using enoxacin as a representative dummy template molecule of fluoroquinolones. The method was optimized concerning the pH of the medium and composite concentration. The normalized fluorescence intensity showed efficient quenching under optimized conditions upon successive addition of the template, with an excellent correlation coefficient. The proposed method was applied to eight other fluoroquinolones, exhibiting, in all cases, good correlation coefficients (0.65–0.992) within the same linearity range (0.03–2.60 mg mL−1). Excellent detection and quantification limits were been obtained for the target analytes down to 0.062 and 0.186 mg L−1, respectively. All studied analytes showed no interference with enrofloxacin, the most commonly used veterinary fluoroquinolone, with a percentage of cross-reactivity varying from 89.00 to 540.00%. This method was applied successfully for the determination of enrofloxacin in three different types of meat samples: beef, pork, and chicken, with good recoveries varying from 70 to 100% at three levels. This new procedure is an easy analytical method that can be useful as a screening method for monitoring the environmental hazard of fluoroquinolones in quality control laboratories.</jats:p>
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-11-07
2023
2023-11-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/104809
url https://hdl.handle.net/20.500.14352/104809
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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