High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells

5 figures.-- Supporting information available.

Detalles Bibliográficos
Autores: Sancho‐Albero, María, Jarne, Carmen, Savirón, María, Martín-Duque, Pilar, Membrado, Luis, Cebolla, Vicente L., Ricote, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/258618
Acceso en línea:http://hdl.handle.net/10261/258618
Access Level:acceso abierto
Palabra clave:HPTLC-MS
Scanning densitometry
Exosomes
Phospholipids
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cellsSancho‐Albero, MaríaJarne, CarmenSavirón, MaríaMartín-Duque, PilarMembrado, LuisCebolla, Vicente L.Ricote, J.HPTLC-MSScanning densitometryExosomesPhospholipidshttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all ages5 figures.-- Supporting information available.The question of whether exosome lipids can be considered as potential cancer biomarkers faces our current limited knowledge of their composition. This is due to the difficulty in isolating pure exosomes, the variability of the biological sources from which they are extracted, and the uncertainty of the methods for lipid characterization. Here, we present a procedure to isolate exosomes and obtain a deep, repeatable, and rapid phospholipid (PL) composition of their lipid extracts, from embryonic murine fibroblasts (NIH-3T3 cell line) and none (B16-F1) and high (B16-F10) metastatic murine skin melanoma cells. The analytical method is based on High Performance Thin-Layer Chromatography with Ultraviolet and fluorescence densitometry and coupled to Electrospray (ESI)-tandem Mass Spectrometry (MS). Under the conditions described in this work, separation and determination of PL classes, (sphingomyelins, SM; phosphatidylcholines, PC; phosphatidylserines, PS; and phosphatidylethanolamines, PE) were achieved, expressed as µg PL/100 µg exosome protein, obtained by bicinchoninic acid assay (BCA). A detailed structural characterization of molecular species of each PL class was performed by simultaneous positive and negative ESI-MS and MS/MS directly from the chromatographic plate, thanks to an elution-based interface.Financial support for this project was provided by DGA-FEDER (E25_20R, N&SB), AES PI21/00036, CSIC 202180E076, ISCIII PI19/01007, DGA E25-20R, and ERC Advanced Grant CADENCE (grant No. ERC-2016-ADG-742684).Peer reviewedMultidisciplinary Digital Publishing InstituteGobierno de AragónEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Instituto de Salud Carlos IIIEuropean Research CouncilJarne, Carmen [0000-0002-9047-7069]Savirón, María [0000-0001-7299-0187]Martín Duque, Pilar [0000-0003-2890-7846]Membrado, Luis [0000-0001-6435-7525]Cebolla, Vicente L. [0000-0002-9786-9217]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/258618reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/DGA/Grupos de Investigación de Referencia Reconocidos 2020-2022/E25_20Rinfo:eu-repo/grantAgreement/ISCIII/Acción Estratégica en Salud 2017-2020/PI21info:eu-repo/grantAgreement/ISCIII/Acción Estratégica en Salud 2017-2020/PI19info:eu-repo/grantAgreement/EC/H2020/742684http://dx.doi.org/10.3390/ijms23031150Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2586182026-05-22T06:33:51Z
dc.title.none.fl_str_mv High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
title High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
spellingShingle High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
Sancho‐Albero, María
HPTLC-MS
Scanning densitometry
Exosomes
Phospholipids
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
title_full High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
title_fullStr High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
title_full_unstemmed High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
title_sort High-performance thin-layer chromatography-densitometry-tandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells
dc.creator.none.fl_str_mv Sancho‐Albero, María
Jarne, Carmen
Savirón, María
Martín-Duque, Pilar
Membrado, Luis
Cebolla, Vicente L.
Ricote, J.
author Sancho‐Albero, María
author_facet Sancho‐Albero, María
Jarne, Carmen
Savirón, María
Martín-Duque, Pilar
Membrado, Luis
Cebolla, Vicente L.
Ricote, J.
author_role author
author2 Jarne, Carmen
Savirón, María
Martín-Duque, Pilar
Membrado, Luis
Cebolla, Vicente L.
Ricote, J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gobierno de Aragón
European Commission
Consejo Superior de Investigaciones Científicas (España)
Instituto de Salud Carlos III
European Research Council
Jarne, Carmen [0000-0002-9047-7069]
Savirón, María [0000-0001-7299-0187]
Martín Duque, Pilar [0000-0003-2890-7846]
Membrado, Luis [0000-0001-6435-7525]
Cebolla, Vicente L. [0000-0002-9786-9217]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HPTLC-MS
Scanning densitometry
Exosomes
Phospholipids
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic HPTLC-MS
Scanning densitometry
Exosomes
Phospholipids
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description 5 figures.-- Supporting information available.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/258618
url http://hdl.handle.net/10261/258618
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/DGA/Grupos de Investigación de Referencia Reconocidos 2020-2022/E25_20R
info:eu-repo/grantAgreement/ISCIII/Acción Estratégica en Salud 2017-2020/PI21
info:eu-repo/grantAgreement/ISCIII/Acción Estratégica en Salud 2017-2020/PI19
info:eu-repo/grantAgreement/EC/H2020/742684
http://dx.doi.org/10.3390/ijms23031150
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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