The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics

Background: The Black Sea is the largest brackish water body in the world, although it is connected to the Mediterranean Sea and presents an upper water layer similar to some regions of the former, albeit with lower salinity and temperature. Despite its well-known hydrology and physicochemical featu...

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Autores: Cabello Yeves, Pedro J., Callieri, Cristiana, Picazo, Antonio, Mehrshad, Maliheh, Haro Moreno, Jose M., Roda García, Juan J., Dzhembekova, Nina, Slabakova, Violeta, Slabakova, Nataliya, Moncheva, Snejana, Rodríguez Valera, Francisco
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dnet:rediumh_____::aff88a8483ff6e05d5ccc1e023539ad1
Acceso en línea:https://hdl.handle.net/11000/39840
Access Level:acceso abierto
Palabra clave:Black Sea microbiota
Genome-resolved metagenomic
Redoxcline
Euxinic waters
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spelling The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomicsCabello Yeves, Pedro J.Callieri, CristianaPicazo, AntonioMehrshad, MalihehHaro Moreno, Jose M.Roda García, Juan J.Dzhembekova, NinaSlabakova, VioletaSlabakova, NataliyaMoncheva, SnejanaRodríguez Valera, FranciscoBlack Sea microbiotaGenome-resolved metagenomicRedoxclineEuxinic watersBackground: The Black Sea is the largest brackish water body in the world, although it is connected to the Mediterranean Sea and presents an upper water layer similar to some regions of the former, albeit with lower salinity and temperature. Despite its well-known hydrology and physicochemical features, this enormous water mass remains poorly studied at the microbial genomics level. Results: We have sampled its different water masses and analyzed the microbiome by shotgun and genomeresolved metagenomics, generating a large number of metagenome-assembled genomes (MAGs) from them. We found various similarities with previously described Black Sea metagenomic datasets, that show remarkable stability in its microbiome. Our datasets are also comparable to other marine anoxic water columns like the Cariaco Basin. The oxic zone resembles to standard marine (e.g. Mediterranean) photic zones, with Cyanobacteria (Synechococcus but a conspicuously absent Prochlorococcus), and photoheterotrophs domination (largely again with marine relatives). The chemocline presents very different characteristics from the oxic surface with many examples of chemolithotrophic metabolism (Thioglobus) and facultatively anaerobic microbes. The euxinic anaerobic zone presents, as expected, features in common with the bottom of meromictic lakes with a massive dominance of sulfate reduction as energy-generating metabolism, a few (but detectable) methanogenesis marker genes, and a large number of “dark matter” streamlined genomes of largely unpredictable ecology. Conclusions: The Black Sea oxic zone presents many similarities to the global ocean while the redoxcline and euxinic water masses have similarities to other similar aquatic environments of marine (Cariaco Basin or other Black Sea regions) or freshwater (meromictic monimolimnion strata) origin. The MAG collection represents very well the different types of metabolisms expected in this kind of environment. We are adding critical information about this unique and important ecosystem and its microbiomeBioMed CentralDepartamentos de la UMH::Producción Vegetal y Microbiología202620262021info:eu-repo/semantics/articleapplication/pdf15application/pdfhttps://hdl.handle.net/11000/39840reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1186/s40793-021-00374-1info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dnet:rediumh_____::aff88a8483ff6e05d5ccc1e023539ad12026-05-27T13:36:21Z
dc.title.none.fl_str_mv The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
title The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
spellingShingle The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
Cabello Yeves, Pedro J.
Black Sea microbiota
Genome-resolved metagenomic
Redoxcline
Euxinic waters
title_short The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
title_full The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
title_fullStr The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
title_full_unstemmed The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
title_sort The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics
dc.creator.none.fl_str_mv Cabello Yeves, Pedro J.
Callieri, Cristiana
Picazo, Antonio
Mehrshad, Maliheh
Haro Moreno, Jose M.
Roda García, Juan J.
Dzhembekova, Nina
Slabakova, Violeta
Slabakova, Nataliya
Moncheva, Snejana
Rodríguez Valera, Francisco
author Cabello Yeves, Pedro J.
author_facet Cabello Yeves, Pedro J.
Callieri, Cristiana
Picazo, Antonio
Mehrshad, Maliheh
Haro Moreno, Jose M.
Roda García, Juan J.
Dzhembekova, Nina
Slabakova, Violeta
Slabakova, Nataliya
Moncheva, Snejana
Rodríguez Valera, Francisco
author_role author
author2 Callieri, Cristiana
Picazo, Antonio
Mehrshad, Maliheh
Haro Moreno, Jose M.
Roda García, Juan J.
Dzhembekova, Nina
Slabakova, Violeta
Slabakova, Nataliya
Moncheva, Snejana
Rodríguez Valera, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Producción Vegetal y Microbiología
dc.subject.none.fl_str_mv Black Sea microbiota
Genome-resolved metagenomic
Redoxcline
Euxinic waters
topic Black Sea microbiota
Genome-resolved metagenomic
Redoxcline
Euxinic waters
description Background: The Black Sea is the largest brackish water body in the world, although it is connected to the Mediterranean Sea and presents an upper water layer similar to some regions of the former, albeit with lower salinity and temperature. Despite its well-known hydrology and physicochemical features, this enormous water mass remains poorly studied at the microbial genomics level. Results: We have sampled its different water masses and analyzed the microbiome by shotgun and genomeresolved metagenomics, generating a large number of metagenome-assembled genomes (MAGs) from them. We found various similarities with previously described Black Sea metagenomic datasets, that show remarkable stability in its microbiome. Our datasets are also comparable to other marine anoxic water columns like the Cariaco Basin. The oxic zone resembles to standard marine (e.g. Mediterranean) photic zones, with Cyanobacteria (Synechococcus but a conspicuously absent Prochlorococcus), and photoheterotrophs domination (largely again with marine relatives). The chemocline presents very different characteristics from the oxic surface with many examples of chemolithotrophic metabolism (Thioglobus) and facultatively anaerobic microbes. The euxinic anaerobic zone presents, as expected, features in common with the bottom of meromictic lakes with a massive dominance of sulfate reduction as energy-generating metabolism, a few (but detectable) methanogenesis marker genes, and a large number of “dark matter” streamlined genomes of largely unpredictable ecology. Conclusions: The Black Sea oxic zone presents many similarities to the global ocean while the redoxcline and euxinic water masses have similarities to other similar aquatic environments of marine (Cariaco Basin or other Black Sea regions) or freshwater (meromictic monimolimnion strata) origin. The MAG collection represents very well the different types of metabolisms expected in this kind of environment. We are adding critical information about this unique and important ecosystem and its microbiome
publishDate 2021
dc.date.none.fl_str_mv 2021
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/39840
url https://hdl.handle.net/11000/39840
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1186/s40793-021-00374-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
15
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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