Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.

San-Roman, B. (Beatriz)|||/items/65a686f1-4bd2-47b8-b238-7c064014090a; Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173; Gomez, S. (Sara)|||/items/95a5744f-419a-4736-8eb3-7a426cb8ab2e; Tsapis, N. (Nicolás)|||/items/4c5141ba-e37b-4e57-bfb3-333f05157cab; Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d; Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c

Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.

It seems well established that CpG oligonucleotides Th1 biased adjuvant activity can be improved when closely associated with a variety of antigens in, for example, microparticles. In this context, we prepared 1-μm near non-charged PLGA 502 or PLGA 756 microparticles that loaded with high efficiency...

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Detalhes bibliográficos
Autores:	San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090a, Irache-Garreta, J.M. (Juan Manuel)\|\|\|/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173, Gomez, S. (Sara)\|\|\|/items/95a5744f-419a-4736-8eb3-7a426cb8ab2e, Tsapis, N. (Nicolás)\|\|\|/items/4c5141ba-e37b-4e57-bfb3-333f05157cab, Gamazo-de la Rasilla, C.M. (Carlos Manuel)\|\|\|/items/d6019c54-7915-4611-94c1-b772366dab1d, Espuelas, S. (Socorro)\|\|\|/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
Formato:	artículo
Fecha de publicación:	2008
País:	España
Recursos:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/17366
Acesso em linha:	https://hdl.handle.net/10171/17366
Access Level:	acceso abierto
Palavra-chave:	Microparticles Antigen Immunotherapy CpG sequences Th1/Th2 immune Poly(lactic-co-glycolic) acid

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repository_id_str
spelling	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090aIrache-Garreta, J.M. (Juan Manuel)\|\|\|/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173Gomez, S. (Sara)\|\|\|/items/95a5744f-419a-4736-8eb3-7a426cb8ab2eTsapis, N. (Nicolás)\|\|\|/items/4c5141ba-e37b-4e57-bfb3-333f05157cabGamazo-de la Rasilla, C.M. (Carlos Manuel)\|\|\|/items/d6019c54-7915-4611-94c1-b772366dab1dEspuelas, S. (Socorro)\|\|\|/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964cMicroparticlesAntigenImmunotherapyCpG sequencesTh1/Th2 immunePoly(lactic-co-glycolic) acidIt seems well established that CpG oligonucleotides Th1 biased adjuvant activity can be improved when closely associated with a variety of antigens in, for example, microparticles. In this context, we prepared 1-μm near non-charged PLGA 502 or PLGA 756 microparticles that loaded with high efficiency an antigen (50% ovalbumin (OVA), approximately) into their matrix and CpG-chitosan complexes (near to 20%) onto their surface maintaining OVA and CpG integrity intact. In the intradermal immunization studies, whereas OVA microencapsulated into PLGA 756 alone induced a strong humoral immune response assisted by a very clear Th1 bias (IgG2a/IgG1=0.875) that was decreased by CpG co-delivery (IgG2a/IgG1=0.55), the co-encapsulation of CpG with OVA in PLGA 502 particles significantly improved the antibody response and isotype shifting (IgG2a/IgG1=0.73) in comparison with mice immunized with OVA loaded PLGA 502 (IgG2a/IgG1=0). This improvement was not correlated with the cellular immune response where the effect of co-encapsulated CpG was rather negative (2030.2 pg/mL and 335.3 pg/mL IFN-g for OVA PLGA 502 for OVA CpG PLGA 502, respectively). These results underscore the critical role of polymer nature and microparticle characteristics to show the benefits of coencapsulating CpG motifs in close proximity with an antigen.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20112011-03-3020082008-01-0120082008-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/17366reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/173662026-06-21T12:47:57Z
dc.title.none.fl_str_mv	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
title	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
spellingShingle	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology. San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090a Microparticles Antigen Immunotherapy CpG sequences Th1/Th2 immune Poly(lactic-co-glycolic) acid
title_short	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
title_full	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
title_fullStr	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
title_full_unstemmed	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
title_sort	Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.
dc.creator.none.fl_str_mv	San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090a Irache-Garreta, J.M. (Juan Manuel)\|\|\|/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 Gomez, S. (Sara)\|\|\|/items/95a5744f-419a-4736-8eb3-7a426cb8ab2e Tsapis, N. (Nicolás)\|\|\|/items/4c5141ba-e37b-4e57-bfb3-333f05157cab Gamazo-de la Rasilla, C.M. (Carlos Manuel)\|\|\|/items/d6019c54-7915-4611-94c1-b772366dab1d Espuelas, S. (Socorro)\|\|\|/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author	San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090a
author_facet	San-Roman, B. (Beatriz)\|\|\|/items/65a686f1-4bd2-47b8-b238-7c064014090a Irache-Garreta, J.M. (Juan Manuel)\|\|\|/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 Gomez, S. (Sara)\|\|\|/items/95a5744f-419a-4736-8eb3-7a426cb8ab2e Tsapis, N. (Nicolás)\|\|\|/items/4c5141ba-e37b-4e57-bfb3-333f05157cab Gamazo-de la Rasilla, C.M. (Carlos Manuel)\|\|\|/items/d6019c54-7915-4611-94c1-b772366dab1d Espuelas, S. (Socorro)\|\|\|/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author_role	author
author2	Irache-Garreta, J.M. (Juan Manuel)\|\|\|/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 Gomez, S. (Sara)\|\|\|/items/95a5744f-419a-4736-8eb3-7a426cb8ab2e Tsapis, N. (Nicolás)\|\|\|/items/4c5141ba-e37b-4e57-bfb3-333f05157cab Gamazo-de la Rasilla, C.M. (Carlos Manuel)\|\|\|/items/d6019c54-7915-4611-94c1-b772366dab1d Espuelas, S. (Socorro)\|\|\|/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author2_role	author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Microparticles Antigen Immunotherapy CpG sequences Th1/Th2 immune Poly(lactic-co-glycolic) acid
topic	Microparticles Antigen Immunotherapy CpG sequences Th1/Th2 immune Poly(lactic-co-glycolic) acid
description	It seems well established that CpG oligonucleotides Th1 biased adjuvant activity can be improved when closely associated with a variety of antigens in, for example, microparticles. In this context, we prepared 1-μm near non-charged PLGA 502 or PLGA 756 microparticles that loaded with high efficiency an antigen (50% ovalbumin (OVA), approximately) into their matrix and CpG-chitosan complexes (near to 20%) onto their surface maintaining OVA and CpG integrity intact. In the intradermal immunization studies, whereas OVA microencapsulated into PLGA 756 alone induced a strong humoral immune response assisted by a very clear Th1 bias (IgG2a/IgG1=0.875) that was decreased by CpG co-delivery (IgG2a/IgG1=0.55), the co-encapsulation of CpG with OVA in PLGA 502 particles significantly improved the antibody response and isotype shifting (IgG2a/IgG1=0.73) in comparison with mice immunized with OVA loaded PLGA 502 (IgG2a/IgG1=0). This improvement was not correlated with the cellular immune response where the effect of co-encapsulated CpG was rather negative (2030.2 pg/mL and 335.3 pg/mL IFN-g for OVA PLGA 502 for OVA CpG PLGA 502, respectively). These results underscore the critical role of polymer nature and microparticle characteristics to show the benefits of coencapsulating CpG motifs in close proximity with an antigen.
publishDate	2008
dc.date.none.fl_str_mv	2008 2008-01-01 2008 2008-01-01 2011 2011-03-30
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/17366
url	https://hdl.handle.net/10171/17366
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,198674

Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology.

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