The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet

Methionine (Met) is not only an essential amino acid of aquatic animals, but it also regulates lipid metabolism. To investigate the impacts of dietary Met supplementation on lipid metabolism, oxidative stress (OS), inflammation and apoptosis in marine fish, a feeding trial lasting for eight weeks wa...

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Detalles Bibliográficos
Autores: Yang, Bingqian, Shen, Yuedong, Monroig, Óscar, Zhao, Wenli, Bao, Yangguang, Tao, Shunshun, Jiao, Lefei, Zhou, Qicun, Jin, Min
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/358501
Acceso en línea:http://hdl.handle.net/10261/358501
Access Level:acceso abierto
Palabra clave:Apoptosis
Inflammatory response
Lipid metabolism
Methionine
Oxidative stress
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oai_identifier_str oai:digital.csic.es:10261/358501
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
title The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
spellingShingle The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
Yang, Bingqian
Apoptosis
Inflammatory response
Lipid metabolism
Methionine
Oxidative stress
title_short The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
title_full The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
title_fullStr The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
title_full_unstemmed The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
title_sort The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet
dc.creator.none.fl_str_mv Yang, Bingqian
Shen, Yuedong
Monroig, Óscar
Zhao, Wenli
Bao, Yangguang
Tao, Shunshun
Jiao, Lefei
Zhou, Qicun
Jin, Min
author Yang, Bingqian
author_facet Yang, Bingqian
Shen, Yuedong
Monroig, Óscar
Zhao, Wenli
Bao, Yangguang
Tao, Shunshun
Jiao, Lefei
Zhou, Qicun
Jin, Min
author_role author
author2 Shen, Yuedong
Monroig, Óscar
Zhao, Wenli
Bao, Yangguang
Tao, Shunshun
Jiao, Lefei
Zhou, Qicun
Jin, Min
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Guangdong Provincial Key R&D Programme
Zhejiang University
Ningbo University
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Apoptosis
Inflammatory response
Lipid metabolism
Methionine
Oxidative stress
topic Apoptosis
Inflammatory response
Lipid metabolism
Methionine
Oxidative stress
description Methionine (Met) is not only an essential amino acid of aquatic animals, but it also regulates lipid metabolism. To investigate the impacts of dietary Met supplementation on lipid metabolism, oxidative stress (OS), inflammation and apoptosis in marine fish, a feeding trial lasting for eight weeks was conducted on juvenile black seabream (Acanthopagrus schlegelii) (initial weight 1.61 ± 0.01 g) fed with five different experimental diets: Control, regular fat diet (11% lipid level); HFD, high-fat diet (17% lipid level), HFD contained different contents of Met (5, 10 or 20 g/ kg) named HFD + M1, HFD + M2, and HFD + M3, respectively. Serum biochemical indices results indicated that the contents of triglyceride (TG), total cholesterol (TC), non-esterified fatty acid (NEFA) and low-density lipoprotein cholesterol (LDL-C), as well as the activity of alanine transaminase (ALT), markedly decreased in HFD + M1 group compared to those in the HFD treatment. Results of liver sections indicated that lipid droplets dramatically decreased by dietary Met supplementation than the HFD group. Similar results were reflected in transmission electron microscope (TEM) images. The mRNA-abundant levels of adenosine monophosphate-activated protein kinase α (ampkα) and silent information regulator 1 (sirt1) significantly increased by dietary HFD + M1 than those in the HFD treatment. When comparing to the HFD treatment, the levels of gene expression for lipogenesis pathway genes, specifically sterol regulatory element-binding protein-1c (srebp-1c) and fatty acid synthase (fas) were markedly improved by dietary HFD + M1. In contrast, opposite results were recorded in the mRNA expression levels of lipolysis pathway related genes (pparα, cpt1a, hsl, atgl, lpl) that were down-regulated by the HFD + M1 by promoting related lipogenesis. The transcriptional expression level of srebp-1c was significantly suppressed in HFD + M1 diet. HFD caused oxidative stress, inflammation and apoptosis in the liver reflected in increasing the reactive oxygen species (ROS) and malonaldehyde (MDA) contents, c-Jun N-terminal kinase (jnk) and nuclear factor kappa B (nf-κb) mRNA expression levels, but those physiological stresses could be alleviated by dietary Met supplementation. Specifically, the antioxidant enzyme activities and gene expression levels were notably up-regulated by HFD + M1 group than those in fish fed with HFD. Additionally, compared to the HFD treatment, dietary Met supplementation significantly decreased the transcriptional expression levels of pro-inflammation and pro apoptosis genes, but up-regulated the transcriptional expression of anti-inflammatory cytokine and anti-apoptosis gene. Together, this study elucidates that HFD supplemented with Met can ameliorate hepatic steatosis, oxidative stress, inflammation and apoptosis induced by HFD, confirming that Met can play a vital role in lowing lipid accumulation by regulating lipid metabolism in A. schlegelii fed with HFD.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/358501
url http://hdl.handle.net/10261/358501
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.aquaculture.2023.740306
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat dietYang, BingqianShen, YuedongMonroig, ÓscarZhao, WenliBao, YangguangTao, ShunshunJiao, LefeiZhou, QicunJin, MinApoptosisInflammatory responseLipid metabolismMethionineOxidative stressMethionine (Met) is not only an essential amino acid of aquatic animals, but it also regulates lipid metabolism. To investigate the impacts of dietary Met supplementation on lipid metabolism, oxidative stress (OS), inflammation and apoptosis in marine fish, a feeding trial lasting for eight weeks was conducted on juvenile black seabream (Acanthopagrus schlegelii) (initial weight 1.61 ± 0.01 g) fed with five different experimental diets: Control, regular fat diet (11% lipid level); HFD, high-fat diet (17% lipid level), HFD contained different contents of Met (5, 10 or 20 g/ kg) named HFD + M1, HFD + M2, and HFD + M3, respectively. Serum biochemical indices results indicated that the contents of triglyceride (TG), total cholesterol (TC), non-esterified fatty acid (NEFA) and low-density lipoprotein cholesterol (LDL-C), as well as the activity of alanine transaminase (ALT), markedly decreased in HFD + M1 group compared to those in the HFD treatment. Results of liver sections indicated that lipid droplets dramatically decreased by dietary Met supplementation than the HFD group. Similar results were reflected in transmission electron microscope (TEM) images. The mRNA-abundant levels of adenosine monophosphate-activated protein kinase α (ampkα) and silent information regulator 1 (sirt1) significantly increased by dietary HFD + M1 than those in the HFD treatment. When comparing to the HFD treatment, the levels of gene expression for lipogenesis pathway genes, specifically sterol regulatory element-binding protein-1c (srebp-1c) and fatty acid synthase (fas) were markedly improved by dietary HFD + M1. In contrast, opposite results were recorded in the mRNA expression levels of lipolysis pathway related genes (pparα, cpt1a, hsl, atgl, lpl) that were down-regulated by the HFD + M1 by promoting related lipogenesis. The transcriptional expression level of srebp-1c was significantly suppressed in HFD + M1 diet. HFD caused oxidative stress, inflammation and apoptosis in the liver reflected in increasing the reactive oxygen species (ROS) and malonaldehyde (MDA) contents, c-Jun N-terminal kinase (jnk) and nuclear factor kappa B (nf-κb) mRNA expression levels, but those physiological stresses could be alleviated by dietary Met supplementation. Specifically, the antioxidant enzyme activities and gene expression levels were notably up-regulated by HFD + M1 group than those in fish fed with HFD. Additionally, compared to the HFD treatment, dietary Met supplementation significantly decreased the transcriptional expression levels of pro-inflammation and pro apoptosis genes, but up-regulated the transcriptional expression of anti-inflammatory cytokine and anti-apoptosis gene. Together, this study elucidates that HFD supplemented with Met can ameliorate hepatic steatosis, oxidative stress, inflammation and apoptosis induced by HFD, confirming that Met can play a vital role in lowing lipid accumulation by regulating lipid metabolism in A. schlegelii fed with HFD.This study was supported by the National Natural Science Foundation of China (32273142, 31802303), Guangdong Province Key Research and Development Project (2021B0202050001), Fundamental Research Funds for the Provincial Universities of Zhejiang (SJLY2021007), the Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University and K. C. Wong Magna Fund in Ningbo University.Peer reviewedElsevier BVNational Natural Science Foundation of ChinaGuangdong Provincial Key R&D ProgrammeZhejiang UniversityNingbo UniversityConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/358501reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.aquaculture.2023.740306Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3585012026-05-22T06:33:51Z
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