A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes

Clinical parameters used in type 2 diabetes mellitus (T2D) diagnosis and monitoring such as glycosylated haemoglobin (HbA1c) are often unable to capture important information related to diabetic control and chronic complications. In order to search for additional biomarkers, we performed a pilot stu...

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Autores: Palomino-Schätzlein, Martina, Lamas-Domingo, Rubén, Ciudin, Andreea|||0000-0001-5622-0203, Gutiérrez-Carcedo, Patricia|||0000-0001-8903-2380, Marés, Roso|||0000-0002-7339-0172, Aparicio-Gómez, Carolina|||0000-0002-0845-2365, Hernández, Cristina|||0000-0002-3109-1721, Simó Canonge, Rafael|||0000-0003-0475-3096, Herance, José Raul|||0000-0001-7178-3290
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:252632
Acceso en línea:https://ddd.uab.cat/record/252632
https://dx.doi.org/urn:doi:10.3390/jcm9061619
Access Level:acceso abierto
Palabra clave:Erythrocytes
Type 2 diabetes mellitus
NMR
Metabolomics
Biomarker
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spelling A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 DiabetesPalomino-Schätzlein, MartinaLamas-Domingo, RubénCiudin, Andreea|||0000-0001-5622-0203Gutiérrez-Carcedo, Patricia|||0000-0001-8903-2380Marés, Roso|||0000-0002-7339-0172Aparicio-Gómez, Carolina|||0000-0002-0845-2365Hernández, Cristina|||0000-0002-3109-1721Simó Canonge, Rafael|||0000-0003-0475-3096Herance, José Raul|||0000-0001-7178-3290ErythrocytesType 2 diabetes mellitusNMRMetabolomicsBiomarkerClinical parameters used in type 2 diabetes mellitus (T2D) diagnosis and monitoring such as glycosylated haemoglobin (HbA1c) are often unable to capture important information related to diabetic control and chronic complications. In order to search for additional biomarkers, we performed a pilot study comparing T2D patients with healthy controls matched by age, gender, and weight. By using 1 H-nuclear magnetic resonance (NMR) based metabolomics profiling of red blood cells (RBCs), we found that the metabolic signature of RBCs in T2D subjects differed significantly from non-diabetic controls. Affected metabolites included glutathione, 2,3-bisphophoglycerate, inosinic acid, lactate, 6-phosphogluconate, creatine and adenosine triphosphate (ATP) and several amino acids such as leucine, glycine, alanine, lysine, aspartate, phenylalanine and tyrosine. These results were validated by an independent cohort of T2D and control patients. An analysis of the pathways in which these metabolites were involved showed that energetic and redox metabolism in RBCs were altered in T2D, as well as metabolites transported by RBCs. Taken together, our results revealed that the metabolic profile of RBCs can discriminate healthy controls from T2D patients. Further research is needed to determine whether metabolic fingerprint in RBC could be useful to complement the information obtained from HbA1c and glycemic variability as well as its potential role in the diabetes managementUniversitat Autònoma de Barcelona 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/252632https://dx.doi.org/urn:doi:10.3390/jcm9061619reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16/0206Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-130open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2526322026-06-06T12:50:31Z
dc.title.none.fl_str_mv A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
title A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
spellingShingle A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
Palomino-Schätzlein, Martina
Erythrocytes
Type 2 diabetes mellitus
NMR
Metabolomics
Biomarker
title_short A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
title_full A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
title_fullStr A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
title_full_unstemmed A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
title_sort A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes
dc.creator.none.fl_str_mv Palomino-Schätzlein, Martina
Lamas-Domingo, Rubén
Ciudin, Andreea|||0000-0001-5622-0203
Gutiérrez-Carcedo, Patricia|||0000-0001-8903-2380
Marés, Roso|||0000-0002-7339-0172
Aparicio-Gómez, Carolina|||0000-0002-0845-2365
Hernández, Cristina|||0000-0002-3109-1721
Simó Canonge, Rafael|||0000-0003-0475-3096
Herance, José Raul|||0000-0001-7178-3290
author Palomino-Schätzlein, Martina
author_facet Palomino-Schätzlein, Martina
Lamas-Domingo, Rubén
Ciudin, Andreea|||0000-0001-5622-0203
Gutiérrez-Carcedo, Patricia|||0000-0001-8903-2380
Marés, Roso|||0000-0002-7339-0172
Aparicio-Gómez, Carolina|||0000-0002-0845-2365
Hernández, Cristina|||0000-0002-3109-1721
Simó Canonge, Rafael|||0000-0003-0475-3096
Herance, José Raul|||0000-0001-7178-3290
author_role author
author2 Lamas-Domingo, Rubén
Ciudin, Andreea|||0000-0001-5622-0203
Gutiérrez-Carcedo, Patricia|||0000-0001-8903-2380
Marés, Roso|||0000-0002-7339-0172
Aparicio-Gómez, Carolina|||0000-0002-0845-2365
Hernández, Cristina|||0000-0002-3109-1721
Simó Canonge, Rafael|||0000-0003-0475-3096
Herance, José Raul|||0000-0001-7178-3290
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Erythrocytes
Type 2 diabetes mellitus
NMR
Metabolomics
Biomarker
topic Erythrocytes
Type 2 diabetes mellitus
NMR
Metabolomics
Biomarker
description Clinical parameters used in type 2 diabetes mellitus (T2D) diagnosis and monitoring such as glycosylated haemoglobin (HbA1c) are often unable to capture important information related to diabetic control and chronic complications. In order to search for additional biomarkers, we performed a pilot study comparing T2D patients with healthy controls matched by age, gender, and weight. By using 1 H-nuclear magnetic resonance (NMR) based metabolomics profiling of red blood cells (RBCs), we found that the metabolic signature of RBCs in T2D subjects differed significantly from non-diabetic controls. Affected metabolites included glutathione, 2,3-bisphophoglycerate, inosinic acid, lactate, 6-phosphogluconate, creatine and adenosine triphosphate (ATP) and several amino acids such as leucine, glycine, alanine, lysine, aspartate, phenylalanine and tyrosine. These results were validated by an independent cohort of T2D and control patients. An analysis of the pathways in which these metabolites were involved showed that energetic and redox metabolism in RBCs were altered in T2D, as well as metabolites transported by RBCs. Taken together, our results revealed that the metabolic profile of RBCs can discriminate healthy controls from T2D patients. Further research is needed to determine whether metabolic fingerprint in RBC could be useful to complement the information obtained from HbA1c and glycemic variability as well as its potential role in the diabetes management
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/252632
https://dx.doi.org/urn:doi:10.3390/jcm9061619
url https://ddd.uab.cat/record/252632
https://dx.doi.org/urn:doi:10.3390/jcm9061619
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16/0206
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-130
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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