Micro-computed tomography and histology to explore internal morphology in decapod larvae

Traditionally, the internal morphology of crustacean larvae has been studied using destructive techniques such as dissection and microscopy. The present study combines advances in micro-computed tomography (micro-CT) and histology to study the internal morphology of decapod larvae, using the common...

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Autores: Castejón Bueno, Diego, Alba Tercedor, Javier, Rotllant, Guiomar, Ribes Mora, Enric, Durfort i Coll, Mercè, Guerao, Guillermo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176806
Acceso en línea:https://hdl.handle.net/2445/176806
Access Level:acceso abierto
Palabra clave:Larves
Tomografia
Sistema nerviós
Larvae
Tomography
Nervous system
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spelling Micro-computed tomography and histology to explore internal morphology in decapod larvaeCastejón Bueno, DiegoAlba Tercedor, JavierRotllant, GuiomarRibes Mora, EnricDurfort i Coll, MercèGuerao, GuillermoLarvesTomografiaSistema nerviósLarvaeTomographyNervous systemTraditionally, the internal morphology of crustacean larvae has been studied using destructive techniques such as dissection and microscopy. The present study combines advances in micro-computed tomography (micro-CT) and histology to study the internal morphology of decapod larvae, using the common spider crab (Maja brachydactyla Balss, 1922) as a model and resolving the individual limitations of these techniques. The synergy of micro-CT and histology allows the organs to be easily identified, revealing simultaneously the gross morphology (shape, size, and location) and histological organization (tissue arrangement and cell identification). Micro-CT shows mainly the exoskeleton, musculature, digestive and nervous systems, and secondarily the circulatory and respiratory systems, while histology distinguishes several cell types and confirms the organ identity. Micro-CT resolves a discrepancy in the literature regarding the nervous system of crab larvae. The major changes occur in the metamorphosis to the megalopa stage, specifically the formation of the gastric mill, the shortening of the abdominal nerve cord, the curving of the abdomen beneath the cephalothorax, and the development of functional pereiopods, pleopods, and lamellate gills. The combination of micro-CT and histology provides better results than either one alone.Nature Publishing Group2021202120182021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/176806Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-018-32709-3Scientific Reports, 2018, vol. 8, num. 1, p. 14399https://doi.org/10.1038/s41598-018-32709-3cc-by (c) Castejón, Diego et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1768062026-05-29T05:05:01Z
dc.title.none.fl_str_mv Micro-computed tomography and histology to explore internal morphology in decapod larvae
title Micro-computed tomography and histology to explore internal morphology in decapod larvae
spellingShingle Micro-computed tomography and histology to explore internal morphology in decapod larvae
Castejón Bueno, Diego
Larves
Tomografia
Sistema nerviós
Larvae
Tomography
Nervous system
title_short Micro-computed tomography and histology to explore internal morphology in decapod larvae
title_full Micro-computed tomography and histology to explore internal morphology in decapod larvae
title_fullStr Micro-computed tomography and histology to explore internal morphology in decapod larvae
title_full_unstemmed Micro-computed tomography and histology to explore internal morphology in decapod larvae
title_sort Micro-computed tomography and histology to explore internal morphology in decapod larvae
dc.creator.none.fl_str_mv Castejón Bueno, Diego
Alba Tercedor, Javier
Rotllant, Guiomar
Ribes Mora, Enric
Durfort i Coll, Mercè
Guerao, Guillermo
author Castejón Bueno, Diego
author_facet Castejón Bueno, Diego
Alba Tercedor, Javier
Rotllant, Guiomar
Ribes Mora, Enric
Durfort i Coll, Mercè
Guerao, Guillermo
author_role author
author2 Alba Tercedor, Javier
Rotllant, Guiomar
Ribes Mora, Enric
Durfort i Coll, Mercè
Guerao, Guillermo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Larves
Tomografia
Sistema nerviós
Larvae
Tomography
Nervous system
topic Larves
Tomografia
Sistema nerviós
Larvae
Tomography
Nervous system
description Traditionally, the internal morphology of crustacean larvae has been studied using destructive techniques such as dissection and microscopy. The present study combines advances in micro-computed tomography (micro-CT) and histology to study the internal morphology of decapod larvae, using the common spider crab (Maja brachydactyla Balss, 1922) as a model and resolving the individual limitations of these techniques. The synergy of micro-CT and histology allows the organs to be easily identified, revealing simultaneously the gross morphology (shape, size, and location) and histological organization (tissue arrangement and cell identification). Micro-CT shows mainly the exoskeleton, musculature, digestive and nervous systems, and secondarily the circulatory and respiratory systems, while histology distinguishes several cell types and confirms the organ identity. Micro-CT resolves a discrepancy in the literature regarding the nervous system of crab larvae. The major changes occur in the metamorphosis to the megalopa stage, specifically the formation of the gastric mill, the shortening of the abdominal nerve cord, the curving of the abdomen beneath the cephalothorax, and the development of functional pereiopods, pleopods, and lamellate gills. The combination of micro-CT and histology provides better results than either one alone.
publishDate 2018
dc.date.none.fl_str_mv 2018
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176806
url https://hdl.handle.net/2445/176806
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-32709-3
Scientific Reports, 2018, vol. 8, num. 1, p. 14399
https://doi.org/10.1038/s41598-018-32709-3
dc.rights.none.fl_str_mv cc-by (c) Castejón, Diego et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Castejón, Diego et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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