First report of Pepo aphid-borne yellows virus on watermelon plants in Spain
In 2021, fifty-two watermelon (Citrullus lanatus) samples exhibiting yellowing virus-like symptoms (Figures 1, 2) were collected from thirteen open-field plots in Murcia (southeastern Spain). Total RNA extraction and dot-blot molecular hybridization were performed to detect Cucumber vein yellowing v...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/356804 |
| Acceso en línea: | http://hdl.handle.net/10261/356804 |
| Access Level: | acceso abierto |
| Palabra clave: | PABYV Polerovirus |
| Sumario: | In 2021, fifty-two watermelon (Citrullus lanatus) samples exhibiting yellowing virus-like symptoms (Figures 1, 2) were collected from thirteen open-field plots in Murcia (southeastern Spain). Total RNA extraction and dot-blot molecular hybridization were performed to detect Cucumber vein yellowing virus, Cucurbit aphid-borne yellows virus (CABYV), Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Zucchini yellow mosaic virus (Rabadán et al., 2023), but none of these viruses were detected. Leaf yellowing symptoms may also be confused with plant nutrient deficiency. However, considering the large aphid populations in the affected crops and the inconspicuous symptoms that poleroviruses often cause in affected cucurbits, we sought to check for the possibility of polerovirus infection. Five random samples were subjected to RT-PCR using the polerovirus primers PolGenUp2 and PolGen RT3 (Lotos et al., 2014). For three samples, a 593 bp amplicon was produced, encompassing part of the polerovirus RNA-dependent RNA polymerase (RdRp) and sequenced (STAB VIDA, Portugal). The sequencing results from the three amplicons confirmed the presence of a polerovirus and identified the virus as Pepo aphid-borne yellows virus (PABYV), with 96.7% nucleic acid identity to GenBank Accession No. LN865082, reported from Greece. PABYV is a phloem-restricted RNA plant viruses that is transmitted by aphids and has been detected in West Africa and the Mediterranean, including Greece, Italy and Syria (Lotos et al., 2016; Parrella et al., 2023) |
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