First embryogenic stages of Citrus microspore-derived embryos

6 p.-2 fig.

Detalhes bibliográficos
Autores: Ramírez, Carmen, Chiancone, Benedetta, Testillano, Pilar S., García-Fojeda, Belén, Germanà, María Antonietta, Risueño, María Carmen
Formato: artículo
Fecha de publicación:2003
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/102992
Acesso em linha:http://hdl.handle.net/10261/102992
Access Level:acceso abierto
Palavra-chave:Citrus
Haploids
Microspore embryogenesis
Ultrastructure
Cytochemistry
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repository_id_str
spelling First embryogenic stages of Citrus microspore-derived embryosRamírez, CarmenChiancone, BenedettaTestillano, Pilar S.García-Fojeda, BelénGermanà, María AntoniettaRisueño, María CarmenCitrusHaploidsMicrospore embryogenesisUltrastructureCytochemistry6 p.-2 fig.This work is the first characterization at cellular and subcellular level of the main cellular events occurring in the first stages of microspore embryogenesis. Microspore embryogenesis was induced in two varieties of Citrus clementina (Nules and Monreal). The results showed that one of the most responsive stages for embryogenesis was the vacuolate microspore. Microscopic analysis revealed specific features of the young proembryos still surrounded by the exine:large nuclei, clear areas in the cytoplasm, starch accumulation, and an increase in the thickness of the wall under the exine. Immunogold labelling with JIM 5 antibody showed a high amount of non-esterified pectins in the surrounding cell wall. After exine rupture, different cell types were detected in late proembryos. As embryogenesis proceeded, the normal pattern of development was observed, including heart-shape, torpedo and cotyledonar embryos.The work was supported by the Spanish project DGESIC PB98-0678,CAM 07G/0054/2000 and the Spanish-Italian joint project CSIC/CNR 2001IT0017.Peer reviewedPolish Academy of SciencesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142003info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/102992reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1029922026-05-22T06:33:51Z
dc.title.none.fl_str_mv First embryogenic stages of Citrus microspore-derived embryos
title First embryogenic stages of Citrus microspore-derived embryos
spellingShingle First embryogenic stages of Citrus microspore-derived embryos
Ramírez, Carmen
Citrus
Haploids
Microspore embryogenesis
Ultrastructure
Cytochemistry
title_short First embryogenic stages of Citrus microspore-derived embryos
title_full First embryogenic stages of Citrus microspore-derived embryos
title_fullStr First embryogenic stages of Citrus microspore-derived embryos
title_full_unstemmed First embryogenic stages of Citrus microspore-derived embryos
title_sort First embryogenic stages of Citrus microspore-derived embryos
dc.creator.none.fl_str_mv Ramírez, Carmen
Chiancone, Benedetta
Testillano, Pilar S.
García-Fojeda, Belén
Germanà, María Antonietta
Risueño, María Carmen
author Ramírez, Carmen
author_facet Ramírez, Carmen
Chiancone, Benedetta
Testillano, Pilar S.
García-Fojeda, Belén
Germanà, María Antonietta
Risueño, María Carmen
author_role author
author2 Chiancone, Benedetta
Testillano, Pilar S.
García-Fojeda, Belén
Germanà, María Antonietta
Risueño, María Carmen
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Citrus
Haploids
Microspore embryogenesis
Ultrastructure
Cytochemistry
topic Citrus
Haploids
Microspore embryogenesis
Ultrastructure
Cytochemistry
description 6 p.-2 fig.
publishDate 2003
dc.date.none.fl_str_mv 2003
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/102992
url http://hdl.handle.net/10261/102992
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422609746100224
score 15,812429

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