Modulation-enhanced localization microscopy: using patterned illumination for super-resolution microscopy: from the localization of single molecules to the descripton of nano-structures
This thesis introduces SIMPLE (Structured Illumination based Point Lo- calization Estimator), a novel super-resolution technique in fluorescence microscopy. By combining standing-wave patterned illumination with single-molecule localization microscopy (SMLM), SIMPLE achieves sig- nificant improvemen...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/689018 |
| Acceso en línea: | http://hdl.handle.net/10803/689018 |
| Access Level: | acceso abierto |
| Palabra clave: | Super-resolution Fluorescence microscopy Localization precision Structured illumination Nano-structures Super-resolución Microscopía de fluorescencia Precisión de localización Iluminación estructurada Nanoestructuras 577 |
| Sumario: | This thesis introduces SIMPLE (Structured Illumination based Point Lo- calization Estimator), a novel super-resolution technique in fluorescence microscopy. By combining standing-wave patterned illumination with single-molecule localization microscopy (SMLM), SIMPLE achieves sig- nificant improvements in localization precision. Experimental results demonstrate a 2-fold enhancement in localization precision over a 400 μm2 field of view, validating the practical effectiveness of SIMPLE. Additionally, this thesis presents meLM (Modulation-Enhanced Lo- calization Microscopy) as a broader concept that encompasses techniques like MINFLUX, SIMPLE, and others. The simulations conducted in this study confirm the theoretical predictions of SIMPLE’s improved local- ization precision throughout the field of view. Furthermore, the thesis proposes an extension of SIMPLE for direct size measurement of sparse nano-structures, which has the potential to overcome limitations imposed by SMLM. Although actual size measure- ments have not been performed, this proposal highlights the promising application of SIMPLE in nano-structure sizing experiments. In summary, SIMPLE demonstrates the power of patterned illumi- nation in advancing super-resolution imaging. The thesis establishes SIMPLE as a key technique within the broader framework of meLM, and suggests its potential for precise nano-sizing experiments. |
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