Role of AE2 for pHi regulation in biliary epithelial cells
The Cl(-)/HCO(-) 3anion exchanger 2 (AE2) is known to be involved in intracellular pH (pHi) regulation and transepithelial acid-base transport. Early studies showed that AE2 gene expression is reduced in liver biopsies and blood mononuclear cells from patients with primary biliary cirrhosis (PBC), a...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/36753 |
| Acceso en línea: | https://hdl.handle.net/10171/36753 |
| Access Level: | acceso abierto |
| Palabra clave: | Primary biliary cirrhosis Cholangiocytes Biliary HCO−3 secretion Bile flow Cl−/HCO−3 anion exchange |
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Role of AE2 for pHi regulation in biliary epithelial cellsMedina, J.F. (Juan Francisco)|||/items/128a68d6-535b-4f01-b435-866451a921cfArdura-Fabregat, A. (Alberto)|||/items/8c85a8ec-312d-4ccd-ac56-da5daa70013fLopez-Martinez, M. (María)|||/items/9d988eaf-66c9-4d75-96a8-7e799f7a66b5Concepcion, A.R. (Axel Rolando)|||/items/9d967590-28cf-4a4a-9643-4de765068ec1Primary biliary cirrhosisCholangiocytesBiliary HCO−3 secretionBile flowCl−/HCO−3 anion exchangeThe Cl(-)/HCO(-) 3anion exchanger 2 (AE2) is known to be involved in intracellular pH (pHi) regulation and transepithelial acid-base transport. Early studies showed that AE2 gene expression is reduced in liver biopsies and blood mononuclear cells from patients with primary biliary cirrhosis (PBC), a disease characterized by chronic non-suppurative cholangitis associated with antimitochondrial antibodies (AMA) and other autoimmune phenomena. Microfluorimetric analysis of the Cl(-)/HCO(-) 3 anion exchange (AE) in isolated cholangiocytes showed that the cAMP-stimulated AE activity is diminished in PBC compared to both healthy and diseased controls. More recently, it was found that miR-506 is upregulated in cholangiocytes of PBC patients and that AE2 may be a target of miR-506. Additional evidence for a pathogenic role of AE2 dysregulation in PBC was obtained with Ae2 (-/-) a,b mice, which develop biochemical, histological, and immunologic alterations that resemble PBC (including development of serum AMA). Analysis of HCO(-) 3 transport systems and pHi regulation in cholangiocytes from normal and Ae2 (-/-) a,b mice confirmed that AE2 is the transporter responsible for the Cl(-)/HCO(-) 3exchange in these cells. On the other hand, both Ae2 (+/+) a,b and Ae2 (-/-) a,b mouse cholangiocytes exhibited a Cl(-)-independent bicarbonate transport system, essentially a Na(+)-bicarbonate cotransport (NBC) system, which could contribute to pHi regulation in the absence of AE2.Dadun. Depósito Académico Digital Universidad de Navarra20142014-09-2620142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/36753reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/367532026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Role of AE2 for pHi regulation in biliary epithelial cells |
| title |
Role of AE2 for pHi regulation in biliary epithelial cells |
| spellingShingle |
Role of AE2 for pHi regulation in biliary epithelial cells Medina, J.F. (Juan Francisco)|||/items/128a68d6-535b-4f01-b435-866451a921cf Primary biliary cirrhosis Cholangiocytes Biliary HCO−3 secretion Bile flow Cl−/HCO−3 anion exchange |
| title_short |
Role of AE2 for pHi regulation in biliary epithelial cells |
| title_full |
Role of AE2 for pHi regulation in biliary epithelial cells |
| title_fullStr |
Role of AE2 for pHi regulation in biliary epithelial cells |
| title_full_unstemmed |
Role of AE2 for pHi regulation in biliary epithelial cells |
| title_sort |
Role of AE2 for pHi regulation in biliary epithelial cells |
| dc.creator.none.fl_str_mv |
Medina, J.F. (Juan Francisco)|||/items/128a68d6-535b-4f01-b435-866451a921cf Ardura-Fabregat, A. (Alberto)|||/items/8c85a8ec-312d-4ccd-ac56-da5daa70013f Lopez-Martinez, M. (María)|||/items/9d988eaf-66c9-4d75-96a8-7e799f7a66b5 Concepcion, A.R. (Axel Rolando)|||/items/9d967590-28cf-4a4a-9643-4de765068ec1 |
| author |
Medina, J.F. (Juan Francisco)|||/items/128a68d6-535b-4f01-b435-866451a921cf |
| author_facet |
Medina, J.F. (Juan Francisco)|||/items/128a68d6-535b-4f01-b435-866451a921cf Ardura-Fabregat, A. (Alberto)|||/items/8c85a8ec-312d-4ccd-ac56-da5daa70013f Lopez-Martinez, M. (María)|||/items/9d988eaf-66c9-4d75-96a8-7e799f7a66b5 Concepcion, A.R. (Axel Rolando)|||/items/9d967590-28cf-4a4a-9643-4de765068ec1 |
| author_role |
author |
| author2 |
Ardura-Fabregat, A. (Alberto)|||/items/8c85a8ec-312d-4ccd-ac56-da5daa70013f Lopez-Martinez, M. (María)|||/items/9d988eaf-66c9-4d75-96a8-7e799f7a66b5 Concepcion, A.R. (Axel Rolando)|||/items/9d967590-28cf-4a4a-9643-4de765068ec1 |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Primary biliary cirrhosis Cholangiocytes Biliary HCO−3 secretion Bile flow Cl−/HCO−3 anion exchange |
| topic |
Primary biliary cirrhosis Cholangiocytes Biliary HCO−3 secretion Bile flow Cl−/HCO−3 anion exchange |
| description |
The Cl(-)/HCO(-) 3anion exchanger 2 (AE2) is known to be involved in intracellular pH (pHi) regulation and transepithelial acid-base transport. Early studies showed that AE2 gene expression is reduced in liver biopsies and blood mononuclear cells from patients with primary biliary cirrhosis (PBC), a disease characterized by chronic non-suppurative cholangitis associated with antimitochondrial antibodies (AMA) and other autoimmune phenomena. Microfluorimetric analysis of the Cl(-)/HCO(-) 3 anion exchange (AE) in isolated cholangiocytes showed that the cAMP-stimulated AE activity is diminished in PBC compared to both healthy and diseased controls. More recently, it was found that miR-506 is upregulated in cholangiocytes of PBC patients and that AE2 may be a target of miR-506. Additional evidence for a pathogenic role of AE2 dysregulation in PBC was obtained with Ae2 (-/-) a,b mice, which develop biochemical, histological, and immunologic alterations that resemble PBC (including development of serum AMA). Analysis of HCO(-) 3 transport systems and pHi regulation in cholangiocytes from normal and Ae2 (-/-) a,b mice confirmed that AE2 is the transporter responsible for the Cl(-)/HCO(-) 3exchange in these cells. On the other hand, both Ae2 (+/+) a,b and Ae2 (-/-) a,b mouse cholangiocytes exhibited a Cl(-)-independent bicarbonate transport system, essentially a Na(+)-bicarbonate cotransport (NBC) system, which could contribute to pHi regulation in the absence of AE2. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2014-09-26 2014 2014-01-01 2014 2014-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/36753 |
| url |
https://hdl.handle.net/10171/36753 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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