Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens

Background: Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials su...

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Autores: Elias, Lisa, Taengua, Rafael, Frigols Garrido, Belén, Salesa Landete, Beatriz, Serrano Aroca, Ángel
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/4707
Acesso em linha:http://hdl.handle.net/20.500.12466/4707
Access Level:acceso abierto
Palavra-chave:Carbon nanomaterials
Antibacterial activity
Graphene oxide
Carbon nanofibers
LED
Methicillin-resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus epidermidis
Cytotoxicity
Human keratinocyte HaCaT cells
3312 Tecnología de Materiales
3321 Tecnología del Carbón y del Petróleo
3302 Tecnología Bioquímica
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oai_identifier_str oai:riucv.ucv.es:20.500.12466/4707
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spelling Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant PathogensElias, LisaTaengua, RafaelFrigols Garrido, BelénSalesa Landete, BeatrizSerrano Aroca, ÁngelCarbon nanomaterialsAntibacterial activityGraphene oxideCarbon nanofibersLEDMethicillin-resistant Staphylococcus aureusMethicillin-resistant Staphylococcus epidermidisCytotoxicityHuman keratinocyte HaCaT cells3312 Tecnología de Materiales3321 Tecnología del Carbón y del Petróleo3302 Tecnología BioquímicaBackground: Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials such as graphene oxide (GO) and carbon nanofibers (CNFs). On the other hand, light-emitting diodes (LEDs) have shown great potential in a wide range of biomedical applications. Methods: We investigated a nanotechnological strategy consisting of GO or CNFs combined with light-emitting diod (LED) irradiation as novel nanoweapons against two clinically relevant Gram-positive multidrug-resistant pathogens: methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE). The cytotoxicity of GO and CNFs was studied in the presence of human keratinocyte HaCaT cells. Results: GO or CNFs exhibited no cytotoxicity and high antibacterial activity in direct contact with MRSE and MRSA cells. Furthermore, when GO or CNFs were illuminated with LED light, the MRSE and MRSA cells lost viability. The rate of decrease in colony forming units from 0 to 3 h, measured per mL, increased to 98.5 ± 1.6% and 95.8 ± 1.4% for GO and 99.5 ± 0.6% and 99.7 ± 0.2% for CNFs. Conclusions: This combined antimicrobial approach opens up many biomedical research opportunities and provides an enhanced strategy for the prevention and treatment of Gram-positive multidrug-resistant infections.20242024-10-2920192019-07-2320192019-07-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/4707reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/47072026-06-19T08:32:07Z
dc.title.none.fl_str_mv Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
title Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
spellingShingle Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
Elias, Lisa
Carbon nanomaterials
Antibacterial activity
Graphene oxide
Carbon nanofibers
LED
Methicillin-resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus epidermidis
Cytotoxicity
Human keratinocyte HaCaT cells
3312 Tecnología de Materiales
3321 Tecnología del Carbón y del Petróleo
3302 Tecnología Bioquímica
title_short Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
title_full Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
title_fullStr Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
title_full_unstemmed Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
title_sort Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens
dc.creator.none.fl_str_mv Elias, Lisa
Taengua, Rafael
Frigols Garrido, Belén
Salesa Landete, Beatriz
Serrano Aroca, Ángel
author Elias, Lisa
author_facet Elias, Lisa
Taengua, Rafael
Frigols Garrido, Belén
Salesa Landete, Beatriz
Serrano Aroca, Ángel
author_role author
author2 Taengua, Rafael
Frigols Garrido, Belén
Salesa Landete, Beatriz
Serrano Aroca, Ángel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Carbon nanomaterials
Antibacterial activity
Graphene oxide
Carbon nanofibers
LED
Methicillin-resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus epidermidis
Cytotoxicity
Human keratinocyte HaCaT cells
3312 Tecnología de Materiales
3321 Tecnología del Carbón y del Petróleo
3302 Tecnología Bioquímica
topic Carbon nanomaterials
Antibacterial activity
Graphene oxide
Carbon nanofibers
LED
Methicillin-resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus epidermidis
Cytotoxicity
Human keratinocyte HaCaT cells
3312 Tecnología de Materiales
3321 Tecnología del Carbón y del Petróleo
3302 Tecnología Bioquímica
description Background: Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials such as graphene oxide (GO) and carbon nanofibers (CNFs). On the other hand, light-emitting diodes (LEDs) have shown great potential in a wide range of biomedical applications. Methods: We investigated a nanotechnological strategy consisting of GO or CNFs combined with light-emitting diod (LED) irradiation as novel nanoweapons against two clinically relevant Gram-positive multidrug-resistant pathogens: methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE). The cytotoxicity of GO and CNFs was studied in the presence of human keratinocyte HaCaT cells. Results: GO or CNFs exhibited no cytotoxicity and high antibacterial activity in direct contact with MRSE and MRSA cells. Furthermore, when GO or CNFs were illuminated with LED light, the MRSE and MRSA cells lost viability. The rate of decrease in colony forming units from 0 to 3 h, measured per mL, increased to 98.5 ± 1.6% and 95.8 ± 1.4% for GO and 99.5 ± 0.6% and 99.7 ± 0.2% for CNFs. Conclusions: This combined antimicrobial approach opens up many biomedical research opportunities and provides an enhanced strategy for the prevention and treatment of Gram-positive multidrug-resistant infections.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-23
2019
2019-07-23
2024
2024-10-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/4707
url http://hdl.handle.net/20.500.12466/4707
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422587289796608
score 15.812455

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