Characterising phages for the control of pathogenic bacteria associated with bivalve consumption

In the present study, five new bacteriophages (or phages) were characterized, and their efficacy in controlling pathogenic bacteria—Escherichia coli, Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, and Vibrio parahaemolyticus—associated with bi...

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Detalles Bibliográficos
Autores: Costa, Pedro, Pereira, Carla, Oliveira, Vanessa, Gomes, Newton C.M., López Romalde, Jesús, Almeida, Adelaide
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/42385
Acceso en línea:https://hdl.handle.net/10347/42385
Access Level:acceso abierto
Palabra clave:Bacteriophages
Pathogenic bacteria
Bivalve consumption
Phage biocontrol
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spelling Characterising phages for the control of pathogenic bacteria associated with bivalve consumptionCosta, PedroPereira, CarlaOliveira, VanessaGomes, Newton C.M.López Romalde, JesúsAlmeida, AdelaideBacteriophagesPathogenic bacteriaBivalve consumptionPhage biocontrolIn the present study, five new bacteriophages (or phages) were characterized, and their efficacy in controlling pathogenic bacteria—Escherichia coli, Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, and Vibrio parahaemolyticus—associated with bivalve consumption was evaluated. The isolated phages include both siphovirus [vB_EcoS_UALMA_PCEc3 (PCEc3), vB_SeTS_UALMA_PCST1 (PCST1), and vB_VpaS_UALMA_PCVp3 (PCVp3)] and myovirus [vB_SeEM_UALMA_PCSE1 (PCSE1) and vB_AhyM_UALMA_PCAh2 (PCAh2)] morphotypes. Four phages are safe for bacterial control, with only one (PCAh2) showing potential lysogenic characteristics. All phages exhibited a narrow host range, capable of infecting up to six additional bacterial strains besides their original host, and four could infect the host bacteria of other phages. Adsorption rates ranged from 24% and 98% within 1 h. One-step growth assays revealed different latent periods, ranging from 10 to 120 min, and low to average burst sizes, ranging from 7.60 to 83.97 PFU/mL. Generally, increasing the multiplicity of infection (MOI) enhanced phage efficiency significantly. All phages effectively reduced the bacterial load of their respective hosts, achieving maximum reductions between 3.73 and 5.57 log CFU/mL within 10 h of treatment. These results suggest that phage biocontrol can be an effective alternative to combat pathogenic bacteria associated with bivalve consumption.ElsevierUniversidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/42385reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© 2025 The Authors. Published by Elsevier B.V.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/423852026-06-15T12:47:27Z
dc.title.none.fl_str_mv Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
title Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
spellingShingle Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
Costa, Pedro
Bacteriophages
Pathogenic bacteria
Bivalve consumption
Phage biocontrol
title_short Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
title_full Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
title_fullStr Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
title_full_unstemmed Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
title_sort Characterising phages for the control of pathogenic bacteria associated with bivalve consumption
dc.creator.none.fl_str_mv Costa, Pedro
Pereira, Carla
Oliveira, Vanessa
Gomes, Newton C.M.
López Romalde, Jesús
Almeida, Adelaide
author Costa, Pedro
author_facet Costa, Pedro
Pereira, Carla
Oliveira, Vanessa
Gomes, Newton C.M.
López Romalde, Jesús
Almeida, Adelaide
author_role author
author2 Pereira, Carla
Oliveira, Vanessa
Gomes, Newton C.M.
López Romalde, Jesús
Almeida, Adelaide
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)
Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.subject.none.fl_str_mv Bacteriophages
Pathogenic bacteria
Bivalve consumption
Phage biocontrol
topic Bacteriophages
Pathogenic bacteria
Bivalve consumption
Phage biocontrol
description In the present study, five new bacteriophages (or phages) were characterized, and their efficacy in controlling pathogenic bacteria—Escherichia coli, Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, and Vibrio parahaemolyticus—associated with bivalve consumption was evaluated. The isolated phages include both siphovirus [vB_EcoS_UALMA_PCEc3 (PCEc3), vB_SeTS_UALMA_PCST1 (PCST1), and vB_VpaS_UALMA_PCVp3 (PCVp3)] and myovirus [vB_SeEM_UALMA_PCSE1 (PCSE1) and vB_AhyM_UALMA_PCAh2 (PCAh2)] morphotypes. Four phages are safe for bacterial control, with only one (PCAh2) showing potential lysogenic characteristics. All phages exhibited a narrow host range, capable of infecting up to six additional bacterial strains besides their original host, and four could infect the host bacteria of other phages. Adsorption rates ranged from 24% and 98% within 1 h. One-step growth assays revealed different latent periods, ranging from 10 to 120 min, and low to average burst sizes, ranging from 7.60 to 83.97 PFU/mL. Generally, increasing the multiplicity of infection (MOI) enhanced phage efficiency significantly. All phages effectively reduced the bacterial load of their respective hosts, achieving maximum reductions between 3.73 and 5.57 log CFU/mL within 10 h of treatment. These results suggest that phage biocontrol can be an effective alternative to combat pathogenic bacteria associated with bivalve consumption.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/42385
url https://hdl.handle.net/10347/42385
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
© 2025 The Authors. Published by Elsevier B.V.
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
© 2025 The Authors. Published by Elsevier B.V.
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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