No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona
Synanthropic rodents such as the brown rat (Rattus norvegicus) and black rat (Rattus rattus) are a source of disturbance in urban areas and the focus of control programs. Control measures often rely on anticoagulant rodenticides, but their broad use is compromised by the emergence of resistance. Her...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:308680 |
| Acceso en línea: | https://ddd.uab.cat/record/308680 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2024.176321 |
| Access Level: | acceso abierto |
| Palabra clave: | Commensal rodents Pest control Rattus norvegicus Rattus rattus Urban Vkorc1 |
| id |
ES_e422fa3fa10c031741cc73377f2adf01 |
|---|---|
| oai_identifier_str |
oai:ddd.uab.cat:308680 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from BarcelonaRuiz-López, María JoséFranco Cirera, Sandra|||0000-0003-4126-2202Martínez de la Puente, JosuéFerraguti, MartinaMiccolis, EmanuelePetit, RobertBarahona Quintana, Laura|||0009-0004-7373-5603Figuerola Borras, Jordi|||0000-0002-4664-9011Montalvo Porro, Tomas|||0000-0002-9060-3205Commensal rodentsPest controlRattus norvegicusRattus rattusUrbanVkorc1Synanthropic rodents such as the brown rat (Rattus norvegicus) and black rat (Rattus rattus) are a source of disturbance in urban areas and the focus of control programs. Control measures often rely on anticoagulant rodenticides, but their broad use is compromised by the emergence of resistance. Here we studied the prevalence of anticoagulant resistance genotypes in the Vkorc1 gene in rats in the metropolitan area of Barcelona. In this area, part of the management practices to control brown rats include anticoagulant rodenticide use, but rodenticides with different active ingredients are used in rotation. Brown rats were sampled from the sewage system during two periods: from December 2016 to November 2017 when difenacoum and brodifacoum were used, and from August 2021 to July 2022 when bromadiolone was used. Because black rats have just recently been detected in Barcelona, we only studied them during the latter sampling period, with samples obtained from a control action carried out in a green urban area. Exon 3 of the Vkorc1 gene was characterized in both species, while exon 1 was additionally analyzed in black rats. Synonymous mutations, not resulting in amino-acid changes, were found in both exons, indicating no evidence of anticoagulant resistance in the rats of Barcelona. This finding indicates that the current rodent management plan in Barcelona, which involves anticoagulant rotation for brown rats and the use of life capture traps in specific situations for black rats, has helped to prevent the emergence of resistance to anticoagulant rodenticides in rats in Barcelona. Future actions should aim to diversify the control measures included in the rodent management plan.Universitat Autònoma de Barcelona 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/308680https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2024.176321reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3086802026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| title |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| spellingShingle |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona Ruiz-López, María José Commensal rodents Pest control Rattus norvegicus Rattus rattus Urban Vkorc1 |
| title_short |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| title_full |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| title_fullStr |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| title_full_unstemmed |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| title_sort |
No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona |
| dc.creator.none.fl_str_mv |
Ruiz-López, María José Franco Cirera, Sandra|||0000-0003-4126-2202 Martínez de la Puente, Josué Ferraguti, Martina Miccolis, Emanuele Petit, Robert Barahona Quintana, Laura|||0009-0004-7373-5603 Figuerola Borras, Jordi|||0000-0002-4664-9011 Montalvo Porro, Tomas|||0000-0002-9060-3205 |
| author |
Ruiz-López, María José |
| author_facet |
Ruiz-López, María José Franco Cirera, Sandra|||0000-0003-4126-2202 Martínez de la Puente, Josué Ferraguti, Martina Miccolis, Emanuele Petit, Robert Barahona Quintana, Laura|||0009-0004-7373-5603 Figuerola Borras, Jordi|||0000-0002-4664-9011 Montalvo Porro, Tomas|||0000-0002-9060-3205 |
| author_role |
author |
| author2 |
Franco Cirera, Sandra|||0000-0003-4126-2202 Martínez de la Puente, Josué Ferraguti, Martina Miccolis, Emanuele Petit, Robert Barahona Quintana, Laura|||0009-0004-7373-5603 Figuerola Borras, Jordi|||0000-0002-4664-9011 Montalvo Porro, Tomas|||0000-0002-9060-3205 |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Commensal rodents Pest control Rattus norvegicus Rattus rattus Urban Vkorc1 |
| topic |
Commensal rodents Pest control Rattus norvegicus Rattus rattus Urban Vkorc1 |
| description |
Synanthropic rodents such as the brown rat (Rattus norvegicus) and black rat (Rattus rattus) are a source of disturbance in urban areas and the focus of control programs. Control measures often rely on anticoagulant rodenticides, but their broad use is compromised by the emergence of resistance. Here we studied the prevalence of anticoagulant resistance genotypes in the Vkorc1 gene in rats in the metropolitan area of Barcelona. In this area, part of the management practices to control brown rats include anticoagulant rodenticide use, but rodenticides with different active ingredients are used in rotation. Brown rats were sampled from the sewage system during two periods: from December 2016 to November 2017 when difenacoum and brodifacoum were used, and from August 2021 to July 2022 when bromadiolone was used. Because black rats have just recently been detected in Barcelona, we only studied them during the latter sampling period, with samples obtained from a control action carried out in a green urban area. Exon 3 of the Vkorc1 gene was characterized in both species, while exon 1 was additionally analyzed in black rats. Synonymous mutations, not resulting in amino-acid changes, were found in both exons, indicating no evidence of anticoagulant resistance in the rats of Barcelona. This finding indicates that the current rodent management plan in Barcelona, which involves anticoagulant rotation for brown rats and the use of life capture traps in specific situations for black rats, has helped to prevent the emergence of resistance to anticoagulant rodenticides in rats in Barcelona. Future actions should aim to diversify the control measures included in the rodent management plan. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2 2024-01-01 2024 2024-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/308680 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2024.176321 |
| url |
https://ddd.uab.cat/record/308680 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2024.176321 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
| instname_str |
Universitat Autònoma de Barcelona |
| reponame_str |
Dipòsit Digital de Documents de la UAB |
| collection |
Dipòsit Digital de Documents de la UAB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869422564402528256 |
| score |
15.198674 |