3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio

Objectives: The current in vitro study aims to evaluate silk fibroin with and without the addition of graphene as a potential scaffold material for regenerative endodontics. Material and methods: Silk fibroin (SF), Silk fibroin/graphene oxide (SF/GO) and silk fibroin coated with reduced graphene oxi...

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Autores: Pagan, Ana, Llena, Carmen, Forner, Leopoldo, Sanz, José L., Sánchez Bautista, Sonia, Ceballos, Laura, Fuentes, Victoria, Melo, María, Rodríguez Lozano, Francisco Javier, Oñate Sánchez, Ricardo E., López García, Sergio, Aznar Cervantes, Salvador D., García Bernal, David
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Católica San Antonio de Murcia (UCAM)
Repositorio:RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
OAI Identifier:oai:repositorio.ucam.edu:10952/8006
Acceso en línea:http://hdl.handle.net/10952/8006
Access Level:acceso abierto
Palabra clave:Graphene
Regenerative Endodontics
Scaffolds
Human dental pulp stem cell (hDPSC)
Silk fibroin
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spelling 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatioPagan, AnaLlena, CarmenForner, LeopoldoSanz, José L.Sánchez Bautista, SoniaCeballos, LauraFuentes, VictoriaMelo, MaríaRodríguez Lozano, Francisco JavierOñate Sánchez, Ricardo E.López García, SergioAznar Cervantes, Salvador D.García Bernal, DavidGrapheneRegenerative EndodonticsScaffoldsHuman dental pulp stem cell (hDPSC)Silk fibroinObjectives: The current in vitro study aims to evaluate silk fibroin with and without the addition of graphene as a potential scaffold material for regenerative endodontics. Material and methods: Silk fibroin (SF), Silk fibroin/graphene oxide (SF/GO) and silk fibroin coated with reduced graphene oxide (SF/rGO) scaffolds were prepared (n = 30). The microarchitectures and mechanical properties of scaffolds were evaluated using field emission scanning electron microscopy (FESEM), pore size and water uptake, attenuated total reflectance fourier transformed infrared spectroscopy (ATR-FTIR), Raman spectroscopy and mechanical compression tests. Next, the study analyzed the influence of these scaffolds on human dental pulp stem cell (hDPSC) viability, apoptosis or necrosis, cell adhesion, odontogenic differentiation marker expression and mineralized matrix deposition. The data were analyzed with ANOVA complemented with the Tukey post-hoc test (p < 0.005). Results: SEM analysis revealed abundant pores with a size greater than 50 nm on the surface of tested scaffolds, primarily between 50 nm and 600 µm. The average value of water uptake obtained in pure fibroin scaffolds was statistically higher than that of those containing GO or rGO (p < 0.05). ATR-FTIR evidenced that the secondary structures did not present differences between pure fibroin and fibroin coated with graphene oxide, with a similar infrared spectrum in all tested scaffolds. Raman spectroscopy showed a greater number of defects in the links in SF/rGO scaffolds due to the reduction of graphene. In addition, adequate mechanical properties were exhibited by the tested scaffolds. Regarding biological properties, hDPSCs attached to scaffolds were capable of proliferating at a rate similar to the control, without affecting their viability over time. A significant upregulation of ALP, ON and DSPP markers was observed with SF/rGO and SF/GO groups. Finally, SF/GO and SF/rGO promoted a significantly higher mineralization than the control at 21 days. Significance: Data obtained suggested that SF/GO and SF/rGO scaffolds promote hDPSC differentiation at a genetic level, increasing the expression of key osteo/odontogenic markers, and supports the mineralization of the extracellular matrix. However, results from this study are to be interpreted with caution, requiring further in vivo studies to confirm the potential of these scaffolds.Odontología2024info:eu-repo/semantics/articlehttp://hdl.handle.net/10952/8006reponame:RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murciainstname:Universidad Católica San Antonio de Murcia (UCAM)Inglés10.1016/j.dental.2023.12.009info:eu-repo/semantics/openAccessoai:repositorio.ucam.edu:10952/80062026-06-07T18:35:21Z
dc.title.none.fl_str_mv 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
title 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
spellingShingle 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
Pagan, Ana
Graphene
Regenerative Endodontics
Scaffolds
Human dental pulp stem cell (hDPSC)
Silk fibroin
title_short 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
title_full 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
title_fullStr 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
title_full_unstemmed 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
title_sort 3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/ odontogenic differentiatio
dc.creator.none.fl_str_mv Pagan, Ana
Llena, Carmen
Forner, Leopoldo
Sanz, José L.
Sánchez Bautista, Sonia
Ceballos, Laura
Fuentes, Victoria
Melo, María
Rodríguez Lozano, Francisco Javier
Oñate Sánchez, Ricardo E.
López García, Sergio
Aznar Cervantes, Salvador D.
García Bernal, David
author Pagan, Ana
author_facet Pagan, Ana
Llena, Carmen
Forner, Leopoldo
Sanz, José L.
Sánchez Bautista, Sonia
Ceballos, Laura
Fuentes, Victoria
Melo, María
Rodríguez Lozano, Francisco Javier
Oñate Sánchez, Ricardo E.
López García, Sergio
Aznar Cervantes, Salvador D.
García Bernal, David
author_role author
author2 Llena, Carmen
Forner, Leopoldo
Sanz, José L.
Sánchez Bautista, Sonia
Ceballos, Laura
Fuentes, Victoria
Melo, María
Rodríguez Lozano, Francisco Javier
Oñate Sánchez, Ricardo E.
López García, Sergio
Aznar Cervantes, Salvador D.
García Bernal, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Graphene
Regenerative Endodontics
Scaffolds
Human dental pulp stem cell (hDPSC)
Silk fibroin
topic Graphene
Regenerative Endodontics
Scaffolds
Human dental pulp stem cell (hDPSC)
Silk fibroin
description Objectives: The current in vitro study aims to evaluate silk fibroin with and without the addition of graphene as a potential scaffold material for regenerative endodontics. Material and methods: Silk fibroin (SF), Silk fibroin/graphene oxide (SF/GO) and silk fibroin coated with reduced graphene oxide (SF/rGO) scaffolds were prepared (n = 30). The microarchitectures and mechanical properties of scaffolds were evaluated using field emission scanning electron microscopy (FESEM), pore size and water uptake, attenuated total reflectance fourier transformed infrared spectroscopy (ATR-FTIR), Raman spectroscopy and mechanical compression tests. Next, the study analyzed the influence of these scaffolds on human dental pulp stem cell (hDPSC) viability, apoptosis or necrosis, cell adhesion, odontogenic differentiation marker expression and mineralized matrix deposition. The data were analyzed with ANOVA complemented with the Tukey post-hoc test (p < 0.005). Results: SEM analysis revealed abundant pores with a size greater than 50 nm on the surface of tested scaffolds, primarily between 50 nm and 600 µm. The average value of water uptake obtained in pure fibroin scaffolds was statistically higher than that of those containing GO or rGO (p < 0.05). ATR-FTIR evidenced that the secondary structures did not present differences between pure fibroin and fibroin coated with graphene oxide, with a similar infrared spectrum in all tested scaffolds. Raman spectroscopy showed a greater number of defects in the links in SF/rGO scaffolds due to the reduction of graphene. In addition, adequate mechanical properties were exhibited by the tested scaffolds. Regarding biological properties, hDPSCs attached to scaffolds were capable of proliferating at a rate similar to the control, without affecting their viability over time. A significant upregulation of ALP, ON and DSPP markers was observed with SF/rGO and SF/GO groups. Finally, SF/GO and SF/rGO promoted a significantly higher mineralization than the control at 21 days. Significance: Data obtained suggested that SF/GO and SF/rGO scaffolds promote hDPSC differentiation at a genetic level, increasing the expression of key osteo/odontogenic markers, and supports the mineralization of the extracellular matrix. However, results from this study are to be interpreted with caution, requiring further in vivo studies to confirm the potential of these scaffolds.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10952/8006
url http://hdl.handle.net/10952/8006
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.1016/j.dental.2023.12.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
instname:Universidad Católica San Antonio de Murcia (UCAM)
instname_str Universidad Católica San Antonio de Murcia (UCAM)
reponame_str RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
collection RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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