Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi

Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identi...

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Authors: Ávalos Cordero, Francisco Javier, Limón Mirón, María del Carmen, Díaz Sánchez, Violeta
Format: article
Status:Published version
Publication Date:2012
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/36412
Online Access:http://hdl.handle.net/11441/36412
https://doi.org/10.1128/AEM.01552-12
Access Level:Open access
Keyword:Agar culture
Conidiation
Fusarium
Fusarium oxysporums
Fusarium species
High sensitivity
MRNA level
Nitrogen availability
Plant tissues
Polyketide synthase genes
Polyketide synthases
Polyketides
Sequence comparisons
Wild types
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spelling Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroiÁvalos Cordero, Francisco JavierLimón Mirón, María del CarmenDíaz Sánchez, VioletaAgar cultureConidiationFusariumFusarium oxysporumsFusarium speciesHigh sensitivityMRNA levelNitrogen availabilityPlant tissuesPolyketide synthase genesPolyketide synthasesPolyketidesSequence comparisonsWild typesFusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cel- lophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues.American Society for MicrobiologyGenética2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/36412https://doi.org/10.1128/AEM.01552-12reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésApplied and Environmental Microbiology, 78, 7258-7266http://dx.doi.org/10.1128/AEM.01552-12info:eu-repo/semantics/openAccessoai:idus.us.es:11441/364122026-06-17T12:51:07Z
dc.title.none.fl_str_mv Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
title Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
spellingShingle Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
Ávalos Cordero, Francisco Javier
Agar culture
Conidiation
Fusarium
Fusarium oxysporums
Fusarium species
High sensitivity
MRNA level
Nitrogen availability
Plant tissues
Polyketide synthase genes
Polyketide synthases
Polyketides
Sequence comparisons
Wild types
title_short Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
title_full Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
title_fullStr Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
title_full_unstemmed Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
title_sort Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
dc.creator.none.fl_str_mv Ávalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
Díaz Sánchez, Violeta
author Ávalos Cordero, Francisco Javier
author_facet Ávalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
Díaz Sánchez, Violeta
author_role author
author2 Limón Mirón, María del Carmen
Díaz Sánchez, Violeta
author2_role author
author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv Agar culture
Conidiation
Fusarium
Fusarium oxysporums
Fusarium species
High sensitivity
MRNA level
Nitrogen availability
Plant tissues
Polyketide synthase genes
Polyketide synthases
Polyketides
Sequence comparisons
Wild types
topic Agar culture
Conidiation
Fusarium
Fusarium oxysporums
Fusarium species
High sensitivity
MRNA level
Nitrogen availability
Plant tissues
Polyketide synthase genes
Polyketide synthases
Polyketides
Sequence comparisons
Wild types
description Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cel- lophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/36412
https://doi.org/10.1128/AEM.01552-12
url http://hdl.handle.net/11441/36412
https://doi.org/10.1128/AEM.01552-12
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Applied and Environmental Microbiology, 78, 7258-7266
http://dx.doi.org/10.1128/AEM.01552-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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