Doped electrospinned material-guides high efficiency regional bone regeneration

The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3- co-(HEA)2 loaded with 5% wt of SiO2 -nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical b...

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Autores: Toledano, Manuel, Vallecillo, Cristina, Serrera Figallo, María de los Ángeles, Vallecillo-Rivas, Marta, Gutiérrez Corrales, Aída, Lynch, Christopher D., Toledano-Osorio, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/160691
Acceso en línea:https://hdl.handle.net/11441/160691
https://doi.org/10.3390/polym15071726
Access Level:acceso abierto
Palabra clave:Zinc
Doxycycline
Silica
Regeneration
Bone cells
Polymer
Membrane
Vascularization
Macrophage
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spelling Doped electrospinned material-guides high efficiency regional bone regenerationToledano, ManuelVallecillo, CristinaSerrera Figallo, María de los ÁngelesVallecillo-Rivas, MartaGutiérrez Corrales, AídaLynch, Christopher D.Toledano-Osorio, ManuelZincDoxycyclineSilicaRegenerationBone cellsPolymerMembraneVascularizationMacrophageThe main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3- co-(HEA)2 loaded with 5% wt of SiO2 -nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and then they were covered with the membranes. After six weeks, a histological analysis (toluidine blue technique) was employed to determine bone cell population as osteoblasts, osteoclasts, osteocytes, M1 and M2 macrophages and vasculature. Membranes covering the bone defect determined a higher count of bone cells and blood vessels than in the sham group at the top regions of the defect. Pro-inflammatory M1 appeared in a higher number in the top regions than in the bottom regions, when Si-M and Dox-Si-M were used. Samples treated with Dox-Si-M showed a higher amount of anti-inflammatory and pro-regenerative M2 macrophages. The M1/M2 ratio obtained its lowest value in the absence of membranes. On the top regions, osteoblasts were more abundant when using Si-M and Zn-Si-M. Osteoclasts were equally distributed at the central and lateral regions. The sham group and samples treated with Zn-Si-M attained a higher number of osteocytes at the top regions. A preferential osteoconductive, osteoinductive and angiogenic clinical environment was created in the vicinity of the membrane placed on critical bone defects.MDPIEstomatología2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/160691https://doi.org/10.3390/polym15071726reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlymers, 15 (7), 1726.https://www.mdpi.com/2073-4360/15/7/1726info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1606912026-06-17T12:51:07Z
dc.title.none.fl_str_mv Doped electrospinned material-guides high efficiency regional bone regeneration
title Doped electrospinned material-guides high efficiency regional bone regeneration
spellingShingle Doped electrospinned material-guides high efficiency regional bone regeneration
Toledano, Manuel
Zinc
Doxycycline
Silica
Regeneration
Bone cells
Polymer
Membrane
Vascularization
Macrophage
title_short Doped electrospinned material-guides high efficiency regional bone regeneration
title_full Doped electrospinned material-guides high efficiency regional bone regeneration
title_fullStr Doped electrospinned material-guides high efficiency regional bone regeneration
title_full_unstemmed Doped electrospinned material-guides high efficiency regional bone regeneration
title_sort Doped electrospinned material-guides high efficiency regional bone regeneration
dc.creator.none.fl_str_mv Toledano, Manuel
Vallecillo, Cristina
Serrera Figallo, María de los Ángeles
Vallecillo-Rivas, Marta
Gutiérrez Corrales, Aída
Lynch, Christopher D.
Toledano-Osorio, Manuel
author Toledano, Manuel
author_facet Toledano, Manuel
Vallecillo, Cristina
Serrera Figallo, María de los Ángeles
Vallecillo-Rivas, Marta
Gutiérrez Corrales, Aída
Lynch, Christopher D.
Toledano-Osorio, Manuel
author_role author
author2 Vallecillo, Cristina
Serrera Figallo, María de los Ángeles
Vallecillo-Rivas, Marta
Gutiérrez Corrales, Aída
Lynch, Christopher D.
Toledano-Osorio, Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Estomatología
dc.subject.none.fl_str_mv Zinc
Doxycycline
Silica
Regeneration
Bone cells
Polymer
Membrane
Vascularization
Macrophage
topic Zinc
Doxycycline
Silica
Regeneration
Bone cells
Polymer
Membrane
Vascularization
Macrophage
description The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3- co-(HEA)2 loaded with 5% wt of SiO2 -nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and then they were covered with the membranes. After six weeks, a histological analysis (toluidine blue technique) was employed to determine bone cell population as osteoblasts, osteoclasts, osteocytes, M1 and M2 macrophages and vasculature. Membranes covering the bone defect determined a higher count of bone cells and blood vessels than in the sham group at the top regions of the defect. Pro-inflammatory M1 appeared in a higher number in the top regions than in the bottom regions, when Si-M and Dox-Si-M were used. Samples treated with Dox-Si-M showed a higher amount of anti-inflammatory and pro-regenerative M2 macrophages. The M1/M2 ratio obtained its lowest value in the absence of membranes. On the top regions, osteoblasts were more abundant when using Si-M and Zn-Si-M. Osteoclasts were equally distributed at the central and lateral regions. The sham group and samples treated with Zn-Si-M attained a higher number of osteocytes at the top regions. A preferential osteoconductive, osteoinductive and angiogenic clinical environment was created in the vicinity of the membrane placed on critical bone defects.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/160691
https://doi.org/10.3390/polym15071726
url https://hdl.handle.net/11441/160691
https://doi.org/10.3390/polym15071726
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plymers, 15 (7), 1726.
https://www.mdpi.com/2073-4360/15/7/1726
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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