Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis

Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related solely to the decre...

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Autores: Parra-Robert, Marina, Casals, Eudald, Massana, Nuria, Zeng, Muling, Perramón, Meritxell, Fernández-Varo, Guillermo, Morales-Ruiz, Manuel, Puntes, Víctor F., Jiménez, Wladimiro, Casals, Gregori
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/200156
Acceso en línea:http://hdl.handle.net/10261/200156
Access Level:acceso abierto
Palabra clave:Nonalcoholic fatty liver disease
Steatosis
Cerium oxide nanoparticles
Oxidative stress
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spelling Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosisParra-Robert, MarinaCasals, EudaldMassana, NuriaZeng, MulingPerramón, MeritxellFernández-Varo, GuillermoMorales-Ruiz, ManuelPuntes, Víctor F.Jiménez, WladimiroCasals, GregoriNonalcoholic fatty liver diseaseSteatosisCerium oxide nanoparticlesOxidative stressNonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related solely to the decrease in oxidative stress or, in addition, they directly decrease liver fatty acid accumulation. To address this question, in this work, we used an in vitro model of hepatocellular steatosis, exposing HepG2 cells to oleic and palmitic acid. Cell uptake of CeO2NPs and their effect on oxidative stress and viability of hepatic cells cultured with H2O2 were also evaluated. Results show that CeO2NPs were uptaken by HepG2 cells and reduced oxidative stress and improved cell viability. Treatment with oleic and palmitic acid increased lipogenesis and the content of different fatty acids. CeO2NPs reduced palmitic and stearic acid and most fatty acids consisting of more than 18 carbon atoms. These effects were associated with significant changes in elongase and desaturase activity. In conclusion, CeO2NPs directly protected HepG2 cells from cell injury in oxidative stress conditions and reduced fatty acid content in steatotic conditions by inducing specific changes in fatty acid metabolism, thus showing potential in the treatment of NAFLD.This research was funded by Ministerio de Economía y Competitividad, grant number PI15-00077 to G.C. and SAF2016-75358-R to M.M-R., co-financed by FEDER, European Union, “A way of making Europe”.; “Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya, convocatòria d’Indústria del Coneixement modalitat B”, grant number 2018_PROD_00187 toW.J, cofinanced by the European Union through the European Regional Development Fund (ERDF), “A way of making Europe”.; CIBERehd is financed by the Instituto de Salud Carlos III.; Wuyi University Funding for Hight Talents Introduction, grant number 2018TP010 to E.C. and 2018TP011 to M.Z.; Foundation from Department of Education of Guangdong Province, grant number 2016KCXTD005 and 2017KSYS010, to E.C. and M.Z. The APC was funded by Wuyi University Funding for Hight Talents Introduction, grant number 2018TP010 to E.C.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)European CommissionGeneralitat de CatalunyaInstituto de Salud Carlos IIINatural Science Foundation of Guangdong ProvinceWuyi UniversityConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/200156reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75358-Rhttps://doi.org/10.3390/biom9090425Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2001562026-05-22T06:33:51Z
dc.title.none.fl_str_mv Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
title Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
spellingShingle Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
Parra-Robert, Marina
Nonalcoholic fatty liver disease
Steatosis
Cerium oxide nanoparticles
Oxidative stress
title_short Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
title_full Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
title_fullStr Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
title_full_unstemmed Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
title_sort Beyond the scavenging of reactive oxygen species (ROS): Direct effect of cerium oxide nanoparticles in reducing fatty acids content in an In vitro model of hepatocellular steatosis
dc.creator.none.fl_str_mv Parra-Robert, Marina
Casals, Eudald
Massana, Nuria
Zeng, Muling
Perramón, Meritxell
Fernández-Varo, Guillermo
Morales-Ruiz, Manuel
Puntes, Víctor F.
Jiménez, Wladimiro
Casals, Gregori
author Parra-Robert, Marina
author_facet Parra-Robert, Marina
Casals, Eudald
Massana, Nuria
Zeng, Muling
Perramón, Meritxell
Fernández-Varo, Guillermo
Morales-Ruiz, Manuel
Puntes, Víctor F.
Jiménez, Wladimiro
Casals, Gregori
author_role author
author2 Casals, Eudald
Massana, Nuria
Zeng, Muling
Perramón, Meritxell
Fernández-Varo, Guillermo
Morales-Ruiz, Manuel
Puntes, Víctor F.
Jiménez, Wladimiro
Casals, Gregori
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Generalitat de Catalunya
Instituto de Salud Carlos III
Natural Science Foundation of Guangdong Province
Wuyi University
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Nonalcoholic fatty liver disease
Steatosis
Cerium oxide nanoparticles
Oxidative stress
topic Nonalcoholic fatty liver disease
Steatosis
Cerium oxide nanoparticles
Oxidative stress
description Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related solely to the decrease in oxidative stress or, in addition, they directly decrease liver fatty acid accumulation. To address this question, in this work, we used an in vitro model of hepatocellular steatosis, exposing HepG2 cells to oleic and palmitic acid. Cell uptake of CeO2NPs and their effect on oxidative stress and viability of hepatic cells cultured with H2O2 were also evaluated. Results show that CeO2NPs were uptaken by HepG2 cells and reduced oxidative stress and improved cell viability. Treatment with oleic and palmitic acid increased lipogenesis and the content of different fatty acids. CeO2NPs reduced palmitic and stearic acid and most fatty acids consisting of more than 18 carbon atoms. These effects were associated with significant changes in elongase and desaturase activity. In conclusion, CeO2NPs directly protected HepG2 cells from cell injury in oxidative stress conditions and reduced fatty acid content in steatotic conditions by inducing specific changes in fatty acid metabolism, thus showing potential in the treatment of NAFLD.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/200156
url http://hdl.handle.net/10261/200156
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75358-R
https://doi.org/10.3390/biom9090425
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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