Bacterial biofilm functionalization through Bap amyloid engineering
Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which pl...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad Pública de Navarra |
| Repositorio: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/44429 |
| Acceso en línea: | https://hdl.handle.net/2454/44429 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacterial biofilms Staphylococcus aureus Bap proteins |
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Bacterial biofilm functionalization through Bap amyloid engineeringMatilla Cuenca, LeticiaTaglialegna, AgustinaGil Puig, CarmenToledo Arana, AlejandroLasa Uzcudun, ÍñigoValle Turrillas, JaioneBacterial biofilmsStaphylococcus aureusBap proteinsBiofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein of Staphylococcus aureus as a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be change by simply modulating the pH of the medium. Using Bap features, we build a tool for trapping and covalent immobilizing molecules at bacterial cell surface or at the biofilm matrix based on the SpyTag/SpyCatcher system. Finally, we show that the cell wall of several Gram-positive bacteria could be functionalized through the external addition of the recombinant engineered Bap-amyloid domain. Overall, this work shows a simple and modulable system for biofilm functionalization based on the facultative protein Bap. © 2022, The Author(s).This research was supported by grants from the Spanish Ministry of Science and Technology RTI2018-096011-B-I00 to J.V. and PID2020-113494RB-I00 to IL. L.M.-C. was supported by the predoctoral program of the Universidad Pública de Navarra.Springer NatureCiencias de la SaludOsasun Zientziak2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/44429reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096011-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113494RB-I00© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/444292026-06-17T12:41:47Z |
| dc.title.none.fl_str_mv |
Bacterial biofilm functionalization through Bap amyloid engineering |
| title |
Bacterial biofilm functionalization through Bap amyloid engineering |
| spellingShingle |
Bacterial biofilm functionalization through Bap amyloid engineering Matilla Cuenca, Leticia Bacterial biofilms Staphylococcus aureus Bap proteins |
| title_short |
Bacterial biofilm functionalization through Bap amyloid engineering |
| title_full |
Bacterial biofilm functionalization through Bap amyloid engineering |
| title_fullStr |
Bacterial biofilm functionalization through Bap amyloid engineering |
| title_full_unstemmed |
Bacterial biofilm functionalization through Bap amyloid engineering |
| title_sort |
Bacterial biofilm functionalization through Bap amyloid engineering |
| dc.creator.none.fl_str_mv |
Matilla Cuenca, Leticia Taglialegna, Agustina Gil Puig, Carmen Toledo Arana, Alejandro Lasa Uzcudun, Íñigo Valle Turrillas, Jaione |
| author |
Matilla Cuenca, Leticia |
| author_facet |
Matilla Cuenca, Leticia Taglialegna, Agustina Gil Puig, Carmen Toledo Arana, Alejandro Lasa Uzcudun, Íñigo Valle Turrillas, Jaione |
| author_role |
author |
| author2 |
Taglialegna, Agustina Gil Puig, Carmen Toledo Arana, Alejandro Lasa Uzcudun, Íñigo Valle Turrillas, Jaione |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Ciencias de la Salud Osasun Zientziak |
| dc.subject.none.fl_str_mv |
Bacterial biofilms Staphylococcus aureus Bap proteins |
| topic |
Bacterial biofilms Staphylococcus aureus Bap proteins |
| description |
Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein of Staphylococcus aureus as a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be change by simply modulating the pH of the medium. Using Bap features, we build a tool for trapping and covalent immobilizing molecules at bacterial cell surface or at the biofilm matrix based on the SpyTag/SpyCatcher system. Finally, we show that the cell wall of several Gram-positive bacteria could be functionalized through the external addition of the recombinant engineered Bap-amyloid domain. Overall, this work shows a simple and modulable system for biofilm functionalization based on the facultative protein Bap. © 2022, The Author(s). |
| publishDate |
2022 |
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2022 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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https://hdl.handle.net/2454/44429 |
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Inglés |
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Inglés |
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https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0/ |
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Springer Nature |
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Springer Nature |
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