Porcine nasal organoids to model interactions between the swine nasal microbiota and the host

Background Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal or...

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Autores: Bonillo Lopez, Laura, Carmona-Vicente, Noelia, Tarrés Freixas, Ferran, Kochanowski, Karl, Martínez, Jorge, Pérez Maíllo, Mónica, Sibila, Marina, Correa-Fiz, Florencia, Aragon, Virginia
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/4690
Acceso en línea:http://hdl.handle.net/20.500.12327/4690
https://doi.org/10.1186/s40168-025-02088-9
Access Level:acceso abierto
Palabra clave:619
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spelling Porcine nasal organoids to model interactions between the swine nasal microbiota and the hostBonillo Lopez, LauraCarmona-Vicente, NoeliaTarrés Freixas, FerranKochanowski, KarlMartínez, JorgePérez Maíllo, MónicaSibila, MarinaCorrea-Fiz, FlorenciaAragon, Virginia619Background Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes. Results PNOs exhibited similar structure and cell types to the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of Moraxella pluranimalium, Rothia nasimurium, and the pathobiont Glaesserella parasuis for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. M. pluranimalium and G. parasuis strains stimulated the production of proinflammatory cytokines, whereas R. nasimurium induced the production of IFNγ and diminished the proinflammatory effect of the other strains. Conclusions Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies.This work was supported by funding from MICIU (Spanish Government) projects PID2019-106233RB-I00/AEI/https://doi.org/10.13039/501100011033 to VA and FCF and PID2022-138657OB-I00/AEI/https://doi.org/10.13039/501100011033 to VA and MS. LBL is supported by a FPI fellowship (PRE2020-096048/AEI/https://doi.org/10.13039/501100011033) and KK by a Ramón y Cajal contract (RYC2021-033035-I/AEI/https://doi.org/10.13039/501100011033), also from MICIU. Finally, the authors acknowledged the support of Centres de Recerca de Catalunya (CERCA) Program.info:eu-repo/semantics/publishedVersionBioMed CentralProducció AnimalSanitat Animal202520252025info:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/20.500.12327/4690https://doi.org/10.1186/s40168-025-02088-9reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésMicrobiomeMICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-106233RB-I00/ES/FUNCIONALIDAD Y EFECTO DE LA MICROBIOTA NASAL SOBRE LA RESPUESTA INMUNE DE LOS LECHONES/MICINN/Programa Estatal para desarrollar, atraer y retener talento/RYC2021-033035-I/ES/ /MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2022-138657OB-I00/ES/CORE ESENCIAL DE LA MICROBIOTA NASAL Y SU USO PARA EL CONTROL DE LAS ENFERMEDADES RESPIRATORIAS PORCINAS/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/46902026-06-16T08:51:17Z
dc.title.none.fl_str_mv Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
title Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
spellingShingle Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
Bonillo Lopez, Laura
619
title_short Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
title_full Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
title_fullStr Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
title_full_unstemmed Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
title_sort Porcine nasal organoids to model interactions between the swine nasal microbiota and the host
dc.creator.none.fl_str_mv Bonillo Lopez, Laura
Carmona-Vicente, Noelia
Tarrés Freixas, Ferran
Kochanowski, Karl
Martínez, Jorge
Pérez Maíllo, Mónica
Sibila, Marina
Correa-Fiz, Florencia
Aragon, Virginia
author Bonillo Lopez, Laura
author_facet Bonillo Lopez, Laura
Carmona-Vicente, Noelia
Tarrés Freixas, Ferran
Kochanowski, Karl
Martínez, Jorge
Pérez Maíllo, Mónica
Sibila, Marina
Correa-Fiz, Florencia
Aragon, Virginia
author_role author
author2 Carmona-Vicente, Noelia
Tarrés Freixas, Ferran
Kochanowski, Karl
Martínez, Jorge
Pérez Maíllo, Mónica
Sibila, Marina
Correa-Fiz, Florencia
Aragon, Virginia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Background Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes. Results PNOs exhibited similar structure and cell types to the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of Moraxella pluranimalium, Rothia nasimurium, and the pathobiont Glaesserella parasuis for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. M. pluranimalium and G. parasuis strains stimulated the production of proinflammatory cytokines, whereas R. nasimurium induced the production of IFNγ and diminished the proinflammatory effect of the other strains. Conclusions Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4690
https://doi.org/10.1186/s40168-025-02088-9
url http://hdl.handle.net/20.500.12327/4690
https://doi.org/10.1186/s40168-025-02088-9
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microbiome
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-106233RB-I00/ES/FUNCIONALIDAD Y EFECTO DE LA MICROBIOTA NASAL SOBRE LA RESPUESTA INMUNE DE LOS LECHONES/
MICINN/Programa Estatal para desarrollar, atraer y retener talento/RYC2021-033035-I/ES/ /
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2022-138657OB-I00/ES/CORE ESENCIAL DE LA MICROBIOTA NASAL Y SU USO PARA EL CONTROL DE LAS ENFERMEDADES RESPIRATORIAS PORCINAS/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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