PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge

© 2010 Elsevier Inc. All rights reserved.

Detalles Bibliográficos
Autores: Fuentes, Juan, Brinca, Lilia, Guerreiro, Pedro M., Power, Deborah M.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345709
Acceso en línea:http://hdl.handle.net/10261/345709
Access Level:acceso abierto
Palabra clave:Gill Na+/K+-ATPase
Osmoregulation
Pituitary gland
Prolactin
Growth hormone
Teleost
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spelling PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challengeFuentes, JuanBrinca, LiliaGuerreiro, Pedro M.Power, Deborah M.Gill Na+/K+-ATPaseOsmoregulationPituitary glandProlactinGrowth hormoneTeleost© 2010 Elsevier Inc. All rights reserved.The endocrine factors prolactin (PRL) and growth hormone (GH) are believed to have counteracting effects in the adaption of fish to changes in environmental salinity. In order to further investigate this interaction sea bream were challenged with full seawater (SW) or freshwater (FW) for 7 days and the response of pituitary glands cultured in vitro to an osmotic challenge (230, 275 and 320 mOsm/kg) was assessed. In vitro PRL secretion from pituitaries of SW-adapted fish was unaltered in response to an osmotic challenge, while GH secretion increased in the lowest osmolality (230 mOsm/kg). In contrast, both GH and PRL secretion by pituitaries from FW challenged fish was significantly increased (p < 0.01) over that of pituitaries from SW fish at the highest osmolality (320 mOsm/kg). After FW challenge pituitary PRL content and de novo synthesised and released PRL were significantly increased (p < 0.01), while total PRL secretion was not different from SW animals. GH pituitary content decreased in FW animals while total secretion and secretion of de novo synthesised protein were significantly increased (p < 0.01). In addition, after transfer of fish to FW expression of PRL and GH increased 3- and 2-fold, respectively. Despite the increase in PRL expression, no increase in total PRL secretion occurred and although in gills a 2-fold increase in the osmoregulatory marker, Na+/K+-ATPase activity was detected, profound haemodilution and a cumulative mortality of 40% occurred in sea bream placed in FW. Taken together the results suggest that the sea bream pituitary gland fails to respond appropriately to the osmotic challenge caused by low salinity and the physiological response evoked in vivo is not enough to allow this species to withstand and adapt to FW.The present work was funded by the Ministry of Science and Technology (FCT), Portugal, project PRAXIS/P/BIA/13174/98 and pluriannual funding to the Centre of Marine Sciences (CCMar). L. Brinca and P.M. Guerreiro were funded by the Ministry of Science (FCT), Portugal through Grants, 4/5.3/PRODEP/2000 and BPD/9464/2002, respectively.Peer reviewedElsevierFundação para a Ciência e a Tecnologia (Portugal)Fuentes, Juan [0000-0003-0430-8734]202420242010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/345709reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.ygcen.2010.04.005Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3457092026-05-22T06:33:51Z
dc.title.none.fl_str_mv PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
title PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
spellingShingle PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
Fuentes, Juan
Gill Na+/K+-ATPase
Osmoregulation
Pituitary gland
Prolactin
Growth hormone
Teleost
title_short PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
title_full PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
title_fullStr PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
title_full_unstemmed PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
title_sort PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge
dc.creator.none.fl_str_mv Fuentes, Juan
Brinca, Lilia
Guerreiro, Pedro M.
Power, Deborah M.
author Fuentes, Juan
author_facet Fuentes, Juan
Brinca, Lilia
Guerreiro, Pedro M.
Power, Deborah M.
author_role author
author2 Brinca, Lilia
Guerreiro, Pedro M.
Power, Deborah M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
Fuentes, Juan [0000-0003-0430-8734]
dc.subject.none.fl_str_mv Gill Na+/K+-ATPase
Osmoregulation
Pituitary gland
Prolactin
Growth hormone
Teleost
topic Gill Na+/K+-ATPase
Osmoregulation
Pituitary gland
Prolactin
Growth hormone
Teleost
description © 2010 Elsevier Inc. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345709
url http://hdl.handle.net/10261/345709
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ygcen.2010.04.005
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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