The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults

Neonates are more susceptible to infections than adults. This susceptibility is thought to reflect neonates’ qualitative and quantitative defects in the adaptive and innate immune responses. Differential expression of cell surface receptors may result in altered thresholds of neonatal immune cell ac...

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Autores: Zenarruzabeitia Belaustegui, Olatz, Vitallé Andrade, Joana, García Obregón, Susana, Astigarraga Aguirre, María Iciar, Eguizabal Argaiz, Cristina, Santos, Silvia, Simhadri, V. R., Borrego Rabasco, Francisco
Tipo de documento: artigo
Data de publicação:2016
País:España
Recursos:Universidad del País Vasco
Repositório:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/69426
Acesso em linha:http://hdl.handle.net/10810/69426
Access Level:Acceso aberto
Palavra-chave:CD300 receptor family
PBMCs
neonate
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spelling The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adultsZenarruzabeitia Belaustegui, OlatzVitallé Andrade, JoanaGarcía Obregón, SusanaAstigarraga Aguirre, María IciarEguizabal Argaiz, CristinaSantos, SilviaSimhadri, V. R.Borrego Rabasco, FranciscoCD300 receptor familyPBMCsneonateNeonates are more susceptible to infections than adults. This susceptibility is thought to reflect neonates’ qualitative and quantitative defects in the adaptive and innate immune responses. Differential expression of cell surface receptors may result in altered thresholds of neonatal immune cell activation. We determined whether the expression and function of the lipid-binding CD300 family of receptors are different on neonatal immune cells compared to adult immune cells. A multiparametric flow cytometry analysis was performed to determine the expression of CD300 receptors on adult peripheral blood mononuclear cells and neonatal cord blood mononuclear cells. The expression of the CD300a inhibitory receptor was significantly reduced on cells from the newborn adaptive immune system, and neonatal antigen presenting cells exhibited a different CD300 receptors expression pattern. We also found differential LPS-mediated regulation of CD300 receptors expression on adult monocytes compared to cord blood monocytes, and that CD300c and CD300e-mediated activation was quantitatively different in neonatal monocytes. This is the first complete study examining the expression of CD300 receptors on human neonatal immune cells compared with adult immune cells. Significant differences in the expression and function of CD300 receptors may help to explain the peculiarities and distinctness of the neonatal immune responses.This work was supported by grants from “Plan Estatal de I+D+I 2013–2016, ISCIII-Subdirección de Evaluación y Fomento de la Investigación-Fondo Europeo de Desarrollo Regional (FEDER) (PI13/00889); Marie Curie Actions, Career Integration Grant, European Commission (CIG 631674); and SAIOTEK, Departamento de Desarrollo Económico y Competitividad, Gobierno Vasco (SAIO13-PE13BF005)”.Nature Research202420242016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/69426reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1038/srep32693info:eu-repo/semantics/openAccess(cc) 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License.http://creativecommons.org/licenses/by/4.0/oai:addi.ehu.eus:10810/694262026-06-18T09:23:17Z
dc.title.none.fl_str_mv The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
title The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
spellingShingle The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
Zenarruzabeitia Belaustegui, Olatz
CD300 receptor family
PBMCs
neonate
title_short The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
title_full The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
title_fullStr The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
title_full_unstemmed The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
title_sort The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults
dc.creator.none.fl_str_mv Zenarruzabeitia Belaustegui, Olatz
Vitallé Andrade, Joana
García Obregón, Susana
Astigarraga Aguirre, María Iciar
Eguizabal Argaiz, Cristina
Santos, Silvia
Simhadri, V. R.
Borrego Rabasco, Francisco
author Zenarruzabeitia Belaustegui, Olatz
author_facet Zenarruzabeitia Belaustegui, Olatz
Vitallé Andrade, Joana
García Obregón, Susana
Astigarraga Aguirre, María Iciar
Eguizabal Argaiz, Cristina
Santos, Silvia
Simhadri, V. R.
Borrego Rabasco, Francisco
author_role author
author2 Vitallé Andrade, Joana
García Obregón, Susana
Astigarraga Aguirre, María Iciar
Eguizabal Argaiz, Cristina
Santos, Silvia
Simhadri, V. R.
Borrego Rabasco, Francisco
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CD300 receptor family
PBMCs
neonate
topic CD300 receptor family
PBMCs
neonate
description Neonates are more susceptible to infections than adults. This susceptibility is thought to reflect neonates’ qualitative and quantitative defects in the adaptive and innate immune responses. Differential expression of cell surface receptors may result in altered thresholds of neonatal immune cell activation. We determined whether the expression and function of the lipid-binding CD300 family of receptors are different on neonatal immune cells compared to adult immune cells. A multiparametric flow cytometry analysis was performed to determine the expression of CD300 receptors on adult peripheral blood mononuclear cells and neonatal cord blood mononuclear cells. The expression of the CD300a inhibitory receptor was significantly reduced on cells from the newborn adaptive immune system, and neonatal antigen presenting cells exhibited a different CD300 receptors expression pattern. We also found differential LPS-mediated regulation of CD300 receptors expression on adult monocytes compared to cord blood monocytes, and that CD300c and CD300e-mediated activation was quantitatively different in neonatal monocytes. This is the first complete study examining the expression of CD300 receptors on human neonatal immune cells compared with adult immune cells. Significant differences in the expression and function of CD300 receptors may help to explain the peculiarities and distinctness of the neonatal immune responses.
publishDate 2016
dc.date.none.fl_str_mv 2016
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/69426
url http://hdl.handle.net/10810/69426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1038/srep32693
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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