The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system

In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim...

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Autores: Burg, Mirjam van der, Kalina, Tomas, Pérez-Andrés, Martin, Vlkova, Marcela, López-Granados, Eduardo, Blanco, Elena, Bonroy, C., Sousa, Ana Berta, Kienzler, Anne-Kathrin, Wentink, Marjolein, Mejstrikova, Ester, Šinkorova, Vendula, Stuchly, Jan, Zelm, Menno C. van, Orfao, Alberto, Dongen, J. J. M. van
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/203944
Acesso em linha:http://hdl.handle.net/10261/203944
Access Level:acceso abierto
Palavra-chave:Flow cytometric immunophenotyping
Primary immunodeficiencies
Automated gating strategy
Standardization
EuroFlow
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spelling The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid systemBurg, Mirjam van derKalina, TomasPérez-Andrés, MartinVlkova, MarcelaLópez-Granados, EduardoBlanco, ElenaBonroy, C.Sousa, Ana BertaKienzler, Anne-KathrinWentink, MarjoleinMejstrikova, EsterŠinkorova, VendulaStuchly, JanZelm, Menno C. vanOrfao, AlbertoDongen, J. J. M. vanFlow cytometric immunophenotypingPrimary immunodeficienciesAutomated gating strategyStandardizationEuroFlowIn the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymphoid PID (without a need for specificity to diagnose particular PID) and to guide and prioritize further diagnostic modalities and clinical management. The tube composition has been defined in a stepwise manner through several cycles of design-testing-evaluation-redesign in a multicenter setting. Equally important appeared to be the standardized pre-analytical procedures (sample preparation and instrument setup), analytical procedures (immunostaining and data acquisition), the software analysis (a multidimensional view based on a reference database in Infinicyt software), and data interpretation. This standardized EuroFlow concept has been tested on 250 healthy controls and 99 PID patients with defined genetic defects. In addition, an application of new EuroFlow software tools with multidimensional pattern recognition was designed with inclusion of maturation pathways in multidimensional patterns (APS plots). The major advantage of the EuroFlow approach is that data can be fully exchanged between different laboratories in any country of the world, which is especially of interest for the PID field, with generally low numbers of cases per center.The coordination and innovation processes of this study were supported by the EuroFlow Consortium (Chairmen: MvdB and AO). MvZ is supported by Senior Research Fellowship GNT1117687 from the Australian National Health and Medical Research Council. TK and EM were supported by projects 15-28541A from Ministry of Health, LO1604 from Ministry of Education, Youth and Sports and GBP302/12/G101 from Grant Agency of the Czech Republic. MP-A, EB, and AO were supported by a grant from the Junta de Castilla y León (Fondo Social Europeo, ORDEN EDU/346/2013, Valladolid, Spain) and the CB16/12/00400 grant (CIBER/ONC, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, - Madrid, Spain- and FONDOS FEDER), the FIS PI12/00905-FEDER grant (Fondo de Investigación Sanitaria of Instituto de Salud Carlos III, Madrid, Spain) and AP119882013 grant (Fundación Mutua Madrileña, Madrid, Spain).Frontiers MediaEuropean CommissionNational Health and Medical Research Council (Australia)Ministry of Health of the Czech RepublicMinistry of Education, Youth and Sports (Czech Republic)Junta de Castilla y LeónInstituto de Salud Carlos IIIMinisterio de Economía y Competitividad (España)Fundación Mutua MadrileñaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/203944reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fimmu.2019.00246Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2039442026-05-22T06:33:51Z
dc.title.none.fl_str_mv The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
title The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
spellingShingle The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
Burg, Mirjam van der
Flow cytometric immunophenotyping
Primary immunodeficiencies
Automated gating strategy
Standardization
EuroFlow
title_short The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
title_full The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
title_fullStr The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
title_full_unstemmed The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
title_sort The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system
dc.creator.none.fl_str_mv Burg, Mirjam van der
Kalina, Tomas
Pérez-Andrés, Martin
Vlkova, Marcela
López-Granados, Eduardo
Blanco, Elena
Bonroy, C.
Sousa, Ana Berta
Kienzler, Anne-Kathrin
Wentink, Marjolein
Mejstrikova, Ester
Šinkorova, Vendula
Stuchly, Jan
Zelm, Menno C. van
Orfao, Alberto
Dongen, J. J. M. van
author Burg, Mirjam van der
author_facet Burg, Mirjam van der
Kalina, Tomas
Pérez-Andrés, Martin
Vlkova, Marcela
López-Granados, Eduardo
Blanco, Elena
Bonroy, C.
Sousa, Ana Berta
Kienzler, Anne-Kathrin
Wentink, Marjolein
Mejstrikova, Ester
Šinkorova, Vendula
Stuchly, Jan
Zelm, Menno C. van
Orfao, Alberto
Dongen, J. J. M. van
author_role author
author2 Kalina, Tomas
Pérez-Andrés, Martin
Vlkova, Marcela
López-Granados, Eduardo
Blanco, Elena
Bonroy, C.
Sousa, Ana Berta
Kienzler, Anne-Kathrin
Wentink, Marjolein
Mejstrikova, Ester
Šinkorova, Vendula
Stuchly, Jan
Zelm, Menno C. van
Orfao, Alberto
Dongen, J. J. M. van
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
National Health and Medical Research Council (Australia)
Ministry of Health of the Czech Republic
Ministry of Education, Youth and Sports (Czech Republic)
Junta de Castilla y León
Instituto de Salud Carlos III
Ministerio de Economía y Competitividad (España)
Fundación Mutua Madrileña
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Flow cytometric immunophenotyping
Primary immunodeficiencies
Automated gating strategy
Standardization
EuroFlow
topic Flow cytometric immunophenotyping
Primary immunodeficiencies
Automated gating strategy
Standardization
EuroFlow
description In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymphoid PID (without a need for specificity to diagnose particular PID) and to guide and prioritize further diagnostic modalities and clinical management. The tube composition has been defined in a stepwise manner through several cycles of design-testing-evaluation-redesign in a multicenter setting. Equally important appeared to be the standardized pre-analytical procedures (sample preparation and instrument setup), analytical procedures (immunostaining and data acquisition), the software analysis (a multidimensional view based on a reference database in Infinicyt software), and data interpretation. This standardized EuroFlow concept has been tested on 250 healthy controls and 99 PID patients with defined genetic defects. In addition, an application of new EuroFlow software tools with multidimensional pattern recognition was designed with inclusion of maturation pathways in multidimensional patterns (APS plots). The major advantage of the EuroFlow approach is that data can be fully exchanged between different laboratories in any country of the world, which is especially of interest for the PID field, with generally low numbers of cases per center.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/203944
url http://hdl.handle.net/10261/203944
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fimmu.2019.00246
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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